Paste in or upload a list of identifiers for HUMAN proteins (e.g UniProt IDs) - gene IDs for protein coding genes will work as well (e.g. ENSEMBL IDs). Hit "Submit query" and wait a few minutes for the server to work it's magic.
The VirtualPulldown server works by searching the InWeb database for proteins that PHYSICAL interacts with the input proteins (hence the "pulldown" metaphor). Proteins fulfilling the quality filtering criteria (by default rather stringent) will be included in the final network. Summary statistics will be shown and network(s) and meta-data will be offered as an easy download for further analysis (e.g. Using Cytoscape)
Click on the "Submit query" button. If the processing of the query takes more than a few seconds you'll will get the option of supplying your email address and be notified when the job is done.
The VirtualPulldown server has support for a number of advanced options. Typically it is not necessary to set these manually and most users can safely skip this section and proceed to submitting the query.
Protein-Protein interaction confidence cut-off
By default the most optimal cut-off found during the benchmark process of InWeb will be selected. It's possible to completely disable this filter by setting the value to "0.0".
Option XXX:
text text text.
Option YYY:
text text text.
XXX_HUMAN YYY_HUMAN |
ENSG00000166851 ENSG00000156711 ENSG00000087586 ENSG00000138778 ENSG00000088325 ENSG00000123975 ENSG00000169679 ENSG00000170540 ENSG00000117724 ENSG00000126787 ENSG00000108106 ENSG00000143228 ENSG00000072571 ENSG00000117399 ENSG00000091732 ENSG00000128606 ENSG00000129195 ENSG00000089685 ENSG00000035499 ENSG00000186193 ENSG00000134057 ENSG00000092140 ENSG00000121957 ENSG00000174500 ENSG00000101224 |