Submit Data

Please note that SigniSite will only accept characters corresponding to the 20 proteogenic amino acid residues and gaps, i.e. anything other than 'ARNDCQEGHILKMFPSTWYV-' will result in an error message.

Paste a multiple alignment in FASTA format into the field below:

Submit a multiple alignment file in FASTA format directly from your local disk:

Sample data

Sample data for test of server functionality is available. The sample data will reproduce the results used on the 'output format' tab. To load the sample data, simply click the 'Load sample data' button below and 'submit'

HIVdb benchmark data set

Click here to download. Please note that the benchmark data set was compiled from the Stanford University HIV drug resistance database, see "How to Cite the HIV Drug Resistance Database".


Instructions on the meaning of the parameters below, can be found on the 'Instructions' tab.
Exclude gaps from the evaluation
Significance threshold α =
Method for correction for multiple testing
Choose sorting of numerical values
Unique sequence ID for relative numbering
Type of logo
Include all positions in logo
Logo plot title, use quotes and underscore, e.g.: "My_Title"


For security reasons, there is a restriction on maximum 500,000 amino acid residues in the submitted multiple sequence aligment. Should you wish to submit an alignment larger than this, please write us at

Reference / citation / citing SigniSite

For publication of results, please cite [1]
  1. Jessen LE, Hoof I, Lund O, Nielsen M.
    SigniSite: Identification of residue-level genotype-phenotype correlations in protein multiple sequence alignments.
    Nucleic Acids Res. 2013 Jul;41(Web Server issue):W286-91. doi: 10.1093/nar/gkt497. Epub 2013 Jun 12.