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If you need help with the bioinformatics programs, see the "Getting Help" section below the program.
The NetSurfP server predicts the surface accessibility and secondary structure of amino acids in an amino acid sequence.
The method also simultaneously predicts the reliability for each prediction, in the form of a Z-score. The Z-score is related to the surface prediction, and not the secondary structure.
For publication of results, please cite:
A generic method for assignment of reliability scores applied to solvent accessibility predictions.
Bent Petersen, Thomas Nordahl Petersen, Pernille Andersen, Morten Nielsen and Claus Lundegaard1.
BMC Structural Biology 2009, 9:51 doi:10.1186/1472-6807-9-51.
The electronic version of this article is found here: download
The sequences can be input in the following two ways:
>test|something
ELVISLEVER
>test_something
ELVISLEVER
At any time during the wait you may enter your e-mail address and simply leave the window. Your job will continue; you will be notified by e-mail when it has terminated. The e-mail message will contain the URL under which the results are stored; they will remain on the server for 24 hours for you to collect them.
# For publication of results, please cite: # A generic method for assignment of reliability scores applied to solvent accessibility predictions. # Bent Petersen, Thomas Nordahl Petersen, Pernille Andersen, Morten Nielsen and Claus Lundegaard # BMC Structural Biology 2009, 9:51 doi:10.1186/1472-6807-9-51 # # Column 1: Class assignment - B for buried or E for Exposed - Threshold: 25% exposure, but not based on RSA # Column 2: Amino acid # Column 3: Sequence name # Column 4: Amino acid number # Column 5: Relative Surface Accessibility - RSA # Column 6: Absolute Surface Accessibility # Column 7: Z-fit score for RSA prediction # Column 8: Probability for Alpha-Helix # Column 9: Probability for Beta-strand # Column 10: Probability for Coil E A 2WNS.A.1 1 0.777 85.680 1.547 0.003 0.003 0.994 E L 2WNS.A.1 2 0.281 51.451 0.503 0.802 0.014 0.185 E G 2WNS.A.1 3 0.530 41.687 1.558 0.938 0.007 0.055 E P 2WNS.A.1 4 0.549 77.846 2.101 0.938 0.007 0.055 B L 2WNS.A.1 5 0.074 13.531 0.513 0.938 0.007 0.055 B V 2WNS.A.1 6 0.068 10.405 1.064 0.975 0.003 0.022 E T 2WNS.A.1 7 0.538 74.662 2.087 0.975 0.003 0.022 E G 2WNS.A.1 8 0.296 23.335 2.326 0.970 0.001 0.030 B L 2WNS.A.1 9 0.029 5.310 0.164 0.970 0.001 0.030 E Y 2WNS.A.1 10 0.326 69.581 1.270 0.923 0.002 0.076 E D 2WNS.A.1 11 0.764 110.107 1.486 0.782 0.003 0.216 B V 2WNS.A.1 12 0.217 33.307 1.603 0.321 0.003 0.675 E Q 2WNS.A.1 13 0.563 100.516 0.817 0.018 0.019 0.964 B A 2WNS.A.1 14 0.058 6.348 0.220 0.018 0.088 0.893 B F 2WNS.A.1 15 0.088 17.662 0.307 0.022 0.552 0.426 E K 2WNS.A.1 16 0.370 76.150 0.997 0.022 0.552 0.426 E F 2WNS.A.1 17 0.454 91.178 0.204 0.021 0.451 0.528 E G 2WNS.A.1 18 0.387 30.481 -0.578 0.019 0.141 0.840 E D 2WNS.A.1 19 0.574 82.742 0.151 0.019 0.141 0.840 B F 2WNS.A.1 20 0.126 25.288 0.725 0.021 0.756 0.223 E V 2WNS.A.1 21 0.393 60.373 0.761 0.018 0.846 0.136 B L 2WNS.A.1 22 0.155 28.289 0.530 0.018 0.846 0.136 E K 2WNS.A.1 23 0.502 103.323 0.020 0.022 0.552 0.426 B S 2WNS.A.1 24 0.319 37.352 -1.215 0.020 0.205 0.775 E G 2WNS.A.1 25 0.502 39.500 -1.743 0.019 0.141 0.840 E L 2WNS.A.1 26 0.384 70.237 -0.338 0.021 0.279 0.699 E S 2WNS.A.1 27 0.449 52.658 -0.193 0.021 0.451 0.528 B S 2WNS.A.1 28 0.158 18.564 -0.225 0.022 0.359 0.619 E P 2WNS.A.1 29 0.452 64.110 -0.834 0.021 0.279 0.699 B I 2WNS.A.1 30 0.232 42.883 0.867 0.023 0.655 0.322 B Y 2WNS.A.1 31 0.115 24.554 0.056 0.021 0.756 0.223 B I 2WNS.A.1 32 0.163 30.155 1.405 0.021 0.756 0.223 B D 2WNS.A.1 33 0.170 24.439 -1.081 0.074 0.484 0.442 B L 2WNS.A.1 34 0.127 23.180 0.923 0.118 0.150 0.732 E R 2WNS.A.1 35 0.377 86.425 0.324 0.278 0.093 0.628 E G 2WNS.A.1 36 0.367 28.883 0.146 0.354 0.048 0.598 B I 2WNS.A.1 37 0.099 18.389 -0.147 0.354 0.048 0.598 B V 2WNS.A.1 38 0.204 31.293 -1.012 0.257 0.016 0.727 E S 2WNS.A.1 39 0.511 59.831 -0.779 0.058 0.017 0.925 B R 2WNS.A.1 40 0.270 61.807 0.663 0.058 0.017 0.925 B P 2WNS.A.1 41 0.212 30.140 -0.022 0.923 0.002 0.076 E R 2WNS.A.1 42 0.603 138.133 1.212 0.970 0.001 0.030 B L 2WNS.A.1 43 0.095 17.340 0.474 0.970 0.001 0.030 B L 2WNS.A.1 44 0.036 6.518 0.464 0.970 0.001 0.030 E S 2WNS.A.1 45 0.352 41.219 1.396 0.970 0.001 0.030 E Q 2WNS.A.1 46 0.301 53.776 1.768 0.970 0.001 0.030 B V 2WNS.A.1 47 0.020 3.105 1.269 0.970 0.001 0.030 B A 2WNS.A.1 48 0.026 2.898 0.818 0.970 0.001 0.030 E D 2WNS.A.1 49 0.440 63.462 1.892 0.970 0.001 0.030 B I 2WNS.A.1 50 0.165 30.451 1.468 0.970 0.001 0.030 B L 2WNS.A.1 51 0.021 3.790 1.276 0.970 0.001 0.030 B F 2WNS.A.1 52 0.072 14.430 0.645 0.970 0.001 0.030 E Q 2WNS.A.1 53 0.585 104.463 1.979 0.970 0.001 0.030 B T 2WNS.A.1 54 0.179 24.786 1.814 0.970 0.001 0.030 B A 2WNS.A.1 55 0.030 3.284 0.243 0.970 0.001 0.030 E Q 2WNS.A.1 56 0.511 91.318 1.607 0.923 0.002 0.076 E N 2WNS.A.1 57 0.836 122.376 0.971 0.782 0.003 0.216 E A 2WNS.A.1 58 0.422 46.493 0.522 0.321 0.003 0.675 E G 2WNS.A.1 59 0.667 52.501 -1.111 0.016 0.005 0.979 B I 2WNS.A.1 60 0.173 32.060 0.007 0.003 0.003 0.994 E S 2WNS.A.1 61 0.636 74.551 1.229 0.005 0.045 0.951 B F 2WNS.A.1 62 0.086 17.320 1.054 0.004 0.197 0.799 E D 2WNS.A.1 63 0.309 44.556 0.085 0.004 0.514 0.481 B T 2WNS.A.1 64 0.074 10.278 0.703 0.001 0.959 0.040 B V 2WNS.A.1 65 0.018 2.720 0.753 0.001 0.959 0.040 B C 2WNS.A.1 66 0.023 3.159 1.210 0.001 0.959 0.040 B G 2WNS.A.1 67 0.037 2.880 -0.003 0.004 0.616 0.381 B V 2WNS.A.1 68 0.054 8.346 1.111 0.005 0.336 0.660 E P 2WNS.A.1 69 0.282 40.087 0.666 0.018 0.088 0.893 E Y 2WNS.A.1 70 0.300 64.003 -0.940 0.115 0.016 0.868 E T 2WNS.A.1 71 0.350 48.600 -1.088 0.176 0.004 0.820 B A 2WNS.A.1 72 0.082 9.003 -2.119 0.321 0.003 0.675 B L 2WNS.A.1 73 0.111 20.361 0.710 0.802 0.014 0.185 B P 2WNS.A.1 74 0.182 25.868 0.084 0.879 0.010 0.111 B L 2WNS.A.1 75 0.104 19.061 0.708 0.938 0.007 0.055 B A 2WNS.A.1 76 0.018 2.006 0.770 0.975 0.003 0.022 B T 2WNS.A.1 77 0.136 18.905 0.910 0.970 0.001 0.030 B V 2WNS.A.1 78 0.151 23.147 -0.019 0.970 0.001 0.030 B I 2WNS.A.1 79 0.041 7.622 0.643 0.988 0.000 0.012 B C 2WNS.A.1 80 0.019 2.640 0.490 0.970 0.001 0.030 E S 2WNS.A.1 81 0.413 48.357 1.221 0.970 0.001 0.030 E T 2WNS.A.1 82 0.431 59.835 0.411 0.923 0.002 0.076 B N 2WNS.A.1 83 0.175 25.664 0.553 0.600 0.003 0.397 E Q 2WNS.A.1 84 0.850 151.774 -0.347 0.016 0.005 0.979 B I 2WNS.A.1 85 0.089 16.410 0.916 0.005 0.015 0.979 E P 2WNS.A.1 86 0.336 47.664 -0.069 0.004 0.138 0.858 B M 2WNS.A.1 87 0.043 8.504 0.261 0.001 0.900 0.099 B L 2WNS.A.1 88 0.034 6.207 0.142 0.001 0.959 0.040 B I 2WNS.A.1 89 0.059 10.878 0.533 0.001 0.959 0.040 B R 2WNS.A.1 90 0.042 9.641 -0.492 0.001 0.900 0.099 B R 2WNS.A.1 91 0.189 43.327 1.011 0.003 0.718 0.279 E K 2WNS.A.1 92 0.320 65.865 0.874 0.004 0.420 0.576 E E 2WNS.A.1 93 0.658 114.918 1.306 0.019 0.141 0.840 E T 2WNS.A.1 94 0.696 96.535 -0.161 0.018 0.047 0.935 E K 2WNS.A.1 95 0.583 119.944 -0.149 0.018 0.088 0.893 E D 2WNS.A.1 96 0.676 97.484 -0.016 0.020 0.205 0.775 E Y 2WNS.A.1 97 0.362 77.274 -0.164 0.021 0.279 0.699 E G 2WNS.A.1 98 0.371 29.229 -1.731 0.022 0.359 0.619 E T 2WNS.A.1 99 0.459 63.663 -0.194 0.021 0.451 0.528 E K 2WNS.A.1 100 0.449 92.400 -0.525 0.021 0.451 0.528 E R 2WNS.A.1 101 0.379 86.699 0.195 0.023 0.655 0.322 B L 2WNS.A.1 102 0.299 54.802 -0.403 0.018 0.846 0.136 B V 2WNS.A.1 103 0.276 42.452 -0.197 0.018 0.846 0.136 E E 2WNS.A.1 104 0.462 80.676 -0.505 0.021 0.756 0.223 E G 2WNS.A.1 105 0.366 28.788 -1.166 0.022 0.552 0.426 E T 2WNS.A.1 106 0.448 62.110 -0.623 0.022 0.359 0.619 B I 2WNS.A.1 107 0.186 34.392 -0.535 0.020 0.205 0.775 E N 2WNS.A.1 108 0.488 71.458 0.676 0.018 0.088 0.893 E P 2WNS.A.1 109 0.663 94.136 0.880 0.018 0.019 0.964 E G 2WNS.A.1 110 0.579 45.599 -0.767 0.003 0.003 0.994 E E 2WNS.A.1 111 0.357 62.350 1.696 0.005 0.045 0.951 E T 2WNS.A.1 112 0.322 44.620 1.838 0.004 0.616 0.381 B C 2WNS.A.1 113 0.023 3.271 1.424 0.001 0.900 0.099 B L 2WNS.A.1 114 0.024 4.321 1.613 0.006 0.962 0.032 B I 2WNS.A.1 115 0.030 5.642 1.224 0.006 0.962 0.032 B I 2WNS.A.1 116 0.026 4.755 1.277 0.011 0.918 0.071 B E 2WNS.A.1 117 0.075 13.033 0.441 0.169 0.612 0.219 B D 2WNS.A.1 118 0.090 12.926 -0.382 0.352 0.332 0.316 B V 2WNS.A.1 119 0.074 11.328 0.571 0.478 0.309 0.214 B V 2WNS.A.1 120 0.081 12.404 0.647 0.538 0.173 0.289 E T 2WNS.A.1 121 0.308 42.761 0.104 0.455 0.046 0.498 B S 2WNS.A.1 122 0.216 25.315 0.268 0.181 0.016 0.803 B G 2WNS.A.1 123 0.178 14.009 -1.613 0.406 0.004 0.590 E S 2WNS.A.1 124 0.357 41.899 0.250 0.858 0.002 0.139 B S 2WNS.A.1 125 0.127 14.931 0.355 0.970 0.001 0.030 B V 2WNS.A.1 126 0.033 5.103 0.136 0.970 0.001 0.030 E L 2WNS.A.1 127 0.300 54.930 1.469 0.988 0.000 0.012 E E 2WNS.A.1 128 0.280 48.864 1.413 0.970 0.001 0.030 B T 2WNS.A.1 129 0.016 2.247 1.090 0.970 0.001 0.030 B V 2WNS.A.1 130 0.067 10.298 0.451 0.970 0.001 0.030 E E 2WNS.A.1 131 0.531 92.766 2.086 0.988 0.000 0.012 B V 2WNS.A.1 132 0.091 14.033 1.432 0.988 0.000 0.012 B L 2WNS.A.1 133 0.018 3.351 1.132 0.970 0.001 0.030 E Q 2WNS.A.1 134 0.532 94.997 1.970 0.970 0.001 0.030 E K 2WNS.A.1 135 0.738 151.807 1.738 0.858 0.002 0.139 B E 2WNS.A.1 136 0.224 39.115 1.166 0.246 0.004 0.750 E G 2WNS.A.1 137 0.737 58.010 0.770 0.003 0.003 0.994 B L 2WNS.A.1 138 0.074 13.604 -0.358 0.005 0.015 0.979 E K 2WNS.A.1 139 0.548 112.682 1.950 0.004 0.514 0.481 B V 2WNS.A.1 140 0.136 20.872 1.493 0.001 0.959 0.040 B T 2WNS.A.1 141 0.170 23.634 1.173 0.001 0.959 0.040 B D 2WNS.A.1 142 0.118 16.989 0.828 0.001 0.959 0.040 B A 2WNS.A.1 143 0.026 2.821 0.855 0.000 0.983 0.017 B I 2WNS.A.1 144 0.025 4.588 0.960 0.000 0.983 0.017 B V 2WNS.A.1 145 0.020 3.074 1.494 0.000 0.983 0.017 B L 2WNS.A.1 146 0.023 4.266 1.283 0.000 0.983 0.017 B L 2WNS.A.1 147 0.030 5.401 0.830 0.001 0.959 0.040 B D 2WNS.A.1 148 0.125 17.940 0.293 0.002 0.816 0.182 E R 2WNS.A.1 149 0.251 57.548 1.003 0.004 0.197 0.799 E E 2WNS.A.1 150 0.440 76.938 -0.160 0.018 0.047 0.935 E Q 2WNS.A.1 151 0.521 92.979 0.330 0.058 0.017 0.925 E G 2WNS.A.1 152 0.600 47.228 -0.105 0.115 0.016 0.868 B G 2WNS.A.1 153 0.107 8.421 -0.968 0.522 0.016 0.462 E K 2WNS.A.1 154 0.451 92.771 1.183 0.717 0.014 0.269 E D 2WNS.A.1 155 0.631 90.927 1.359 0.802 0.014 0.185 E K 2WNS.A.1 156 0.266 54.716 1.505 0.802 0.014 0.185 B L 2WNS.A.1 157 0.084 15.307 0.078 0.802 0.014 0.185 E Q 2WNS.A.1 158 0.668 119.216 1.422 0.802 0.014 0.185 E A 2WNS.A.1 159 0.746 82.264 0.879 0.600 0.003 0.397 E H 2WNS.A.1 160 0.409 74.379 0.821 0.176 0.004 0.820 E G 2WNS.A.1 161 0.785 61.787 0.218 0.003 0.003 0.994 B I 2WNS.A.1 162 0.076 14.079 0.879 0.005 0.045 0.951 E R 2WNS.A.1 163 0.452 103.508 1.409 0.005 0.262 0.733 B L 2WNS.A.1 164 0.065 11.828 0.672 0.021 0.756 0.223 B H 2WNS.A.1 165 0.243 44.238 1.455 0.011 0.918 0.071 B S 2WNS.A.1 166 0.068 7.958 0.001 0.011 0.918 0.071 B V 2WNS.A.1 167 0.048 7.439 -0.173 0.018 0.846 0.136 B C 2WNS.A.1 168 0.058 8.087 0.981 0.021 0.756 0.223 E T 2WNS.A.1 169 0.346 47.949 1.675 0.003 0.718 0.279 B L 2WNS.A.1 170 0.056 10.217 1.000 0.453 0.248 0.299 E S 2WNS.A.1 171 0.522 61.237 0.956 0.717 0.014 0.269 E K 2WNS.A.1 172 0.405 83.391 1.778 0.717 0.014 0.269 B M 2WNS.A.1 173 0.033 6.563 0.438 0.879 0.010 0.111 B L 2WNS.A.1 174 0.121 22.064 1.180 0.938 0.007 0.055 E E 2WNS.A.1 175 0.580 101.291 1.704 0.970 0.001 0.030 B I 2WNS.A.1 176 0.170 31.505 1.032 0.970 0.001 0.030 B L 2WNS.A.1 177 0.036 6.592 -0.837 0.970 0.001 0.030 E E 2WNS.A.1 178 0.536 93.692 1.427 0.923 0.002 0.076 E Q 2WNS.A.1 179 0.856 152.882 1.365 0.858 0.002 0.139 E Q 2WNS.A.1 180 0.457 81.674 1.388 0.246 0.004 0.750 E K 2WNS.A.1 181 0.602 123.790 -0.107 0.016 0.005 0.979 E K 2WNS.A.1 182 0.545 112.086 1.328 0.018 0.019 0.964 B V 2WNS.A.1 183 0.074 11.374 -0.259 0.018 0.019 0.964 E D 2WNS.A.1 184 0.447 64.384 1.394 0.018 0.019 0.964 E A 2WNS.A.1 185 0.691 76.192 1.387 0.923 0.002 0.076 E E 2WNS.A.1 186 0.729 127.409 1.262 0.858 0.002 0.139 B T 2WNS.A.1 187 0.219 30.389 0.792 0.858 0.002 0.139 B V 2WNS.A.1 188 0.121 18.659 1.241 0.970 0.001 0.030 E G 2WNS.A.1 189 0.669 52.682 1.838 0.970 0.001 0.030 E R 2WNS.A.1 190 0.345 79.074 2.359 0.970 0.001 0.030 B V 2WNS.A.1 191 0.029 4.457 0.484 0.970 0.001 0.030 E K 2WNS.A.1 192 0.443 91.146 1.977 0.970 0.001 0.030 E R 2WNS.A.1 193 0.566 129.568 2.122 0.923 0.002 0.076 B F 2WNS.A.1 194 0.132 26.472 0.674 0.923 0.002 0.076 E I 2WNS.A.1 195 0.339 62.807 0.927 0.782 0.003 0.216 E Q 2WNS.A.1 196 0.776 138.540 1.328 0.502 0.002 0.495 E E 2WNS.A.1 197 0.931 162.576 1.532 0.016 0.005 0.979
1to whom correspondence should be addressed, e-mail:
lunde@cbs.dtu.dk
Center for Biological Sequence Analysis, CBS, Department of Systems Biology.
The Technical University of Denmark, DK-2800 Lyngby, Denmark.
If you need help regarding technical issues (e.g. errors or missing results) contact Technical Support. Please include the name of the service and version (e.g. NetPhos-4.0) and the options you have selected. If the error occurs after the job has started running, please include the JOB ID (the long code that you see while the job is running).
If you have scientific questions (e.g. how the method works or how to interpret results), contact Correspondence.
Correspondence:
Technical Support: