In order to use the NetChop server for prediction on amino acid sequences:
  1. Enter the sequence in the sequence window, or give a file name.

    The sequence must be written using the one letter amino acid code: `acdefghiklmnpqrstvwy' or `ACDEFGHIKLMNPQRSTVWY'.
    Other letters will be converted to `X' and treated as unknown amino acids.
    Other characters, such as whitespace and numbers, will simply be ignored.

  2. (optional) Select prediciton method

  3. Change the threshold: to increase the threshold results in better specificity, but worse sensitivity.

  4. Press the "Submit sequence" button.

  5. A WWW page will return the results when the prediction is ready. Response time depends on system load.