Instructions
In order to use the DictyOglyc server for prediction on amino-acids
sequences either:
- Type the name of the sequence in the 'Sequence name' field
- The sequence must be submitted using the one letter code for the amino
acids:
`acdefghiklmnpqrstvwyACDEFGHIKLMNPQRSTVWY'
- Other characters will be accepted, but not encoded in the network window,
when making the prediction. Do not use these.
- Peptides shorter than 19 residues can produce non-reliable results. (always
include 9 residues on both sides of the Ser/Thr you want evaluated. The
optimal is to use the complete "native" glycoprotein sequence truncating
the signal sequence)
- Click on the "Generate Graph" checkbox if you'd like to see a graph
with the output (Gif/Postscript). These may be quite useful in seeing the
"hot" spots in your protein.
- Press the "Submit sequence" button.
- A WWW page will return the results when the prediction is ready. Response
time depends on system load.
OR
- Use the file browser to find your sequences in FASTA format in your own
appropiate directory (a single file may contain multiple sequences).
Example of two sequences in fasta format:
>seq1
ASTPGHTIIYEAVCLHNDRTTIP
>seq2
ASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPK
NMYKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGR
KSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR
- Click on Generate graph if required, and then press the "Send
file" button.