STEP 1: Input sequences

Paste in one or more DNA sequences in FASTA format Perform DNA fusion, site directed mutagenesis, and combinatorial assembly of sequences. See how in the instructions tab.

or upload DNA sequences in FASTA format

Input is circular (only allows circular output)

STEP 2: Output construct

circular linear into a USER cassette (click to show list)

STEP 3: PCR experimental conditions



Please read the CBS access policies for information about limitations on the daily number of submissions.

The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Hans Jasper Genee, Mads Tvillinggaard Bonde, Frederik Otzen Bagger, Jakob Berg Jespersen, Morten O. A. Sommer, Rasmus Wernersson, and Lars Rønn Olsen
Software-supported USER cloning strategies for site-directed mutagenesis and DNA assembly

Keywords: : DNA assembly, USER cloning, primer design, site-directed mutagenesis, point mutation, web server.