Brief instructions:
Select a complete predefined fusion cassette on the list below - this will provide all the information needed. If you don't need to clone your fused construct into a vector, you can select LINEAR or CIRCULAR, and no cassette will be used

Here you can select a predefined fusion cassette (see details below)

1: Name: PacI/Nt.BbvCI Forward strand: GCTGAGGGTTTAATTAAGACCTCAGC Reverse strand: CGACTCCCAAATTAATTCTGGAGTCG 2: Name: XbaI2/Nt.BbvCI Forward strand: GCTGAGGGAAAGTCTAGAGGATCCTCTAGATGTCTCCTCAGC Reverse strand: CGACTCCCTTTCAGATCTCCTAGGAGATCTACAGAGGAGTCG 3: Name: PmeI/Nb.BbvCI Forward strand: CCTCAGCCGTTTAAACAGCTGAGG Reverse strand: GGAGTCGGCAAATTTGTCGACTCC 4: Name: AsiSI/Nb.BSMI Forward strand: GAATGCGTGCGATCGCGTGCATTC Reverse strand: CTTACGCACGCTAGCGCACGTAAG 5: Name: AsiSI/Nb.BtsI Forward strand: GCAGTGAGAGCGATCGCAGACACTGC Reverse strand: CGTCACTCTCGCTAGCGTCTGTGACG

Submission

STEP 1: Input sequences

Paste in one or more DNA sequences in FASTA format Perform DNA fusion, site directed mutagenesis, and combinatorial assembly of sequences. See how in the instructions tab.

or upload DNA sequences in FASTA format

STEP 2: Output construct

STEP 3: PCR experimental conditions

CITATIONS

Paste in one or more DNA sequences in FASTA format Perform DNA fusion, site directed mutagenesis, and combinatorial assembly of sequences. See how in the instructions tab.
or upload DNA sequences in FASTA format

Input is circular (only allows circular output)