DTU Health Tech
Department of Health Technology
DTU Health Tech
Department of Health Technology
Would you prefer to run MAIT Match at your own site? Mait_Match v. 1.0 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for the most common UNIX platforms. There is a download tab for academic users; other users are requested to contact the Software Package Manager at health-software@dtu.dk.
# Read 21 elements on linelist /home/projects/mniel/MAIT_Match/data/blosum62.qij # Number of PEP entries read 10 from file /usr/opt/www/webface/tmp/server/mait_match/5543C1FC0000566C77B8007C/file.0 # Number of PEP entries read 187 from file /home/projects/mniel/MAIT_Match/data/MAIT_DB_update Res Sequence MAIT_hit Score Best CAMRESISSGSARQLTF CAPSGSARQLTF 0.8331 Best CAANVGGGSNYKLTF CAVPNSGGSNYKLTF 0.8718 Best CAGGDNYGQNFVF CAVQGNYGQNFVF 0.8706 Best CAMRESISSGSARQLTF CAPSGSARQLTF 0.8331 Best CALGELWDQAGTALIF CAVLGQAGTALIF 0.8161 Best CAMSEGGYNKLIF CAVGSGGYNKLI 0.8884 Best CAGAPRDNYGQNFVF CAVEEDNYGQNFVF 0.8308 Best CAVEPGGYQKVTF CVAYSGGYQKVT 0.8595 Best CACDPLGGGDTTDKLIF CAVGEDTGKLIF 0.7544 Best CAVNPNDYKLSF CAVLSNDYKLSF 0.9019
A population of pro-inflammatory TRAV1-2+ CD8+ T-cells including oligoconal expansions of MAIT cells is enriched in the airways in human tuberculosis
Emily B. Wong 1,2, Marielle C. Gold3,4,5, Umesh Lalloo6, Bongiwe Z. Xulu1, Zuri A. Sullivan1, Zoe Rogers1, Henrik Kloverpris1, Erin W. Meermeier5, Prabhat K. Sharma4, Aneta H. Worley 3,4, Prinita Baijnath6, Anish Ambaram6, Leon Naidoo6, Rajhmun Madansein7, James E. McLaren8, David A. Price 8,9, Samuel M. Behar10, Morten Nielsen11, Victoria O. Kasprowicz1,12,13, Alasdair Leslie1, Thumbi Ndung'u1,12,13,14, William R. Bishai15, David M. Lewinsohn3,4,5.
Abstract
Mucosal-associated invariant T (MAIT) cells typically express a TRAV1-2+ semi-invariant TCR that enables recognition of bacterial, mycobacterial, and fungal riboflavin metabolites presented by MR1. MAIT cells are associated with immune control of bacterial and mycobacterial infections in murine models. Here, we report that a population of pro-inflammatory TRAV1-2+ CD8+ T cells are present in the airways and lungs of healthy individuals and are enriched in bronchoalveolar fluid of patients with active pulmonary tuberculosis (TB). High-throughput T cell receptor analysis reveals oligoclonal expansions of canonical and donor-unique TRAV1-2+ MAIT-consistent TCR# sequences within this population. Some of these cells demonstrate MR1-restricted mycobacterial reactivity and phenotypes suggestive of MAIT cell identity. These findings demonstrate enrichment of TRAV1-2+ CD8+ T cells with MAIT or MAIT-like features in the airways during active TB and suggest a role for these cells in the human pulmonary immune response to Mycobacterium tuberculosis.
1KwaZulu-Natal Research Institute for Tuberculosis and HIV, Durban, South Africa
2Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA
3Department of Pulmonary & Critical Care Medicine, Oregon Health & Science University, Portland, OR, USA
4 VA Portland Health Care Center, Portland, OR, USA
5Department of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, Oregon 97239, USA
6Department of Pulmonology and Critical Care, Nelson Mandela School of Medicine, University of KwaZulu Natal, Durban, South Africa
7Department of Cardiothoracic Surgery, Nelson Mandela School of Medicine, University of KwaZulu Natal, Durban, South Africa
8Institute of Infection & Immunity, Cardiff University School of Medicine, Cardiff, Wales, UK
9Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
10Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA
11Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark, Lyngby, Denmark
12 HIV Pathogenesis Programme, University of KwaZulu-Natal, Durban, South Africa
13 The Ragon Institute of MGH, MIT, and Harvard, Harvard Medical School, Cambridge, MA
14Max Planck Institute for Infection Biology, Berlin, Germany
15Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD
CAAEDSNYQLIW CAAESNSGYALNF CAAFDSNYQLIW CAAFDSNYRLIF CAAGGQNFVF CAAIDSNYQLIW CAALDSNYQLIW CAALNSNYQLIW CAALSDYKLSF CAALSNDYKLSF CAAMDSNYQLIW CAANREGAQKLVF CAAPTGRRALTF CAASAAGYGNKLVF CAASDSNYQLIW CAASKAAGNKLTF CAASKSGYSTLT CAASMGYSSASKIIF CAATGNQGAQKLVF CAAVDSNYQLIW CADLSNSGYALNF CAEAQGGTALIF CAEMEIYNQGGKLIF CAETGIHNAGNNRKLIF CAFMDSNYQLIW CAGERAEYGNKLVF CAGGANAGGTSYGKLTF CAGGTGTASKLTF CAGHDRAGSYQLTF CAGLDSNYQLIW CAGMDSNYQLIW CAGPPNGNKLVF CAGWDSNYQLIW CAIIGFGGSQGNLI CAIMDSNYQLIW CAIPQGGSEKLV CALDPRVGGFKTIF CALGENYLPPTGNTPLV CALGHMNRDDKIIF CALGRRSSASKIIF CALGRWSSASKIIF CALLDSNYQLIW CALLTNFGNEKLT CALNDMRF CALRDTGFQKLVF CALSEGPLTGNQFY CAMREADSSYKLIF CAMRERRYSSASKIIF CAMRESYGNNRLAF CAMSINYGGSQGNLI CAPLDSNYQLIW CAPMDSNYQLIW CAPSGSARQLTF CAPVDSSYKLIF CARGMDSSYKLIF CARGYSGGGADGLT CARMDSNYQLIW CARSDSNYQLIW CASEGCGNKLVF CASIDSNYQLIW CASLDSSYKLIF CASMDSNYQLIW CASMDSSYKLIF CASRSYNTDKLIF CASSDSKYQLIW CATMDSNYQLIW CAVAGAPGGSYIPTF CAVDDNTDKLIF CAVEAWNNAGNMLTF CAVEDPQTGANNLF CAVEEDNYGQNFVF CAVEPPIVGKST CAVEPRTSGGSYIPTF CAVEPWTSGGSYIPTF CAVESNSGYALNF CAVFSDGQKLLF CAVGADDYKLSF CAVGDNAGNMLTF CAVGDSNYQLIW CAVGEDTGKLIF CAVGSGGYNKLI CAVGYSSASKIIF CAVIDSNYQLIW CAVIGGFGNVLHC CAVIQGDYKLSF CAVKDSNYQLIW CAVKEGNYQLIW CAVLDGNYQLIW CAVLDSNYQLIW CAVLDSSYKLIF CAVLGQAGTALIF CAVLNAGGFKTIF CAVLNTGGFKTIF CAVLQGDYKLSF CAVLSNAYKLSF CAVLSNDYKLSF CAVMDDNYQLIW CAVMDSNYQLIW CAVMDSSYKLIF CAVMGGYNFNKFYF CAVMGSSYKLIF CAVMRNAGNMLTF CAVNNNNDMRF CAVPFYSSASKIIF CAVPNSGGSNYKLTF CAVPNYNQGGKLIF CAVPREGVDNTDKLIF CAVPSGGSYIPTF CAVQDSNYQLIW CAVQGNYGQNFVF CAVQNSNYQLIW CAVRADNNARLMF CAVRDGAGIKIIF CAVRDGAGNMLTF CAVRDGAGNRLTF CAVRDGDYKLIF CAVRDGDYKLSF CAVRDGDYKPSF CAVRDGHYKLSF CAVRDGNYQLIW CAVRDGTDSWGKLQF CAVRDNDYKLSF CAVRDNFNKFYF CAVRDPDNARLMF CAVRDRDYKLSF CAVRDRDYQLIW CAVRDREYGNKLVF CAVRDRILNYGGATNKLIF CAVRDRLNARLMF CAVRDRNYGGATNKLIF CAVRDRQAGTALIF CAVRDSDYKLSF CAVRDSNYQLIW CAVRDSSYKLIF CAVRDTGFQKLVF CAVRDWTGANNLF CAVRERGDADNMLTF CAVRERGDAGNMLTF CAVRERGGAGNMLTF CAVRESNYQLIW CAVRGATDSWGKLQF CAVRGDNYQLIW CAVRGGDYKLSF CAVRGGWDSNYQLIW CAVRGLTYNTDKLIF CAVRGNFNKFYF CAVRLHDKLIF CAVRLHSNYQLIW CAVRNSNYQLIW CAVRQINTGTASKLTF CAVRRDDKIIF CAVRRRDDKIIF CAVRRYSGAGSYQLTF CAVRSEGARLMF CAVRVDRGSTLGRLYF CAVRVVNNAGNMLTF CAVRVVYNQGGKLIF CAVSDSNYQLIW CAVSDSSYKLIF CAVSGDYKLSF CAVSPEDSNYQLIW CAVTDSNYQLIW CAVTPPSGGSYIPTF CAVVDSNYQLIW CAVVNYGQNFVF CAVWYSWGKLQF CAVYDSNYQLIW CAVYGDMRF CAWLRFQKLVF CGQEEATYLHLEEGRGSYIPTF CILQQDAGGNSYGKLTF CIPLDSSNTGKLIF CIVRFSGTYKYIF CLTRIRANQAGTALIF CLVGDHTGGFKTIF CSNQAGTALIF CVAYSGGYQKVT CVGGPYSGAGSYQLTF CVLVDSNYQLIW CVPMDSNYQLIW CVSVDSNYQLIW CVVKDSGYALNF CVVRDGAGNMLTF CVVRDGNDRWGKLQF CVVSAEQAGTALIF CVVSGSDGQKLLF CVVSSSGTYKYIF
If you need help regarding technical issues (e.g. errors or missing results) contact Technical Support. Please include the name of the service and version (e.g. NetPhos-4.0). If the error occurs after the job has started running, please include the JOB ID (the long code that you see while the job is running).
If you have scientific questions (e.g. how the method works or how to interpret results), contact Correspondence.
Correspondence:
Technical Support:
