NES-accession: NES-0001 Date: 14 Aug 2002 Protein: cAMP-dependent Protein Kinase Inhibitor, alpha form (PKI-alpha). Organism: Homo sapiens (Human) / Oryctolagus cuniculus (Rabbit). DB_reference: SWISS-PROT P04541, IPKA_HUMAN. Necessity: Export abrogated by the following mutations: L37A/L39A/L41A, L37A/L41A, L41A/L44A, L44A/I46A, L44A and I46A. Sufficiency: Aa 37-46 mediates export of GST. Pathway: LMB-sensitive. Location: - Regulation: - Comments: - References: Wen W, Meinkoth JL, Tsien RY and Taylor SS, 1995, Cell 82: 463-73. Medline: 7634336 Henderson and Eleftheriou, 2000, Exp. Cell Res. 256: 213-224. Medline: 10739668 Sequence: 75 aa TDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEEDAQRSSTEQSGEAQGEAAKSES ....................................MaMaMaaMaM............................. // NES-accession: NES-0002 Date: 14 Aug 2002 Protein: Cellular tumor antigen p53 (Tumor suppressor p53) (Phosphoprotein p53) (Antigen NY-CO-13) Organism: Homo sapiens (Human). DB_reference: SWISS-PROT P04637, P53_HUMAN. NLSdb NLS:76, NLS:127 Necessity: L348A/L350A does not shuttle in heterokaryon assay (Stommel et al. 1999) Sufficiency: Aa 339-352 mediates export of BSA (stommel et al. 1999). Zhang and Xiong 2001 further shows aa 11-27 to mediate export of GFP, which is abrogated by the L14Q/F19S or L22Q/W23S mutations. This is argued though, by Inoue et al. 2002, who shows that p53 aa 7-27 cannot function as an autonomous NES. Pathway: LMB-sensitive Location: Nucleus Regulation: Regulation by masking the NES upon tetramerization (Stommel et al. 1999). Phosphorylation of Ser15, abrogates export (Zhang and Xiong 2001) Comments: There is controversy regarding the N-terminal NES (Zhang and Xiong 2001, Inoue et al. 2002), why it has not been included in the assignment field. References: Stommel JM, Marchenko ND, Jimenez GS, Moll UM, Hope TJ and Wahl GM, 1999 EMBO J 18: 1660-72. Medline: 10075936 Zhang Y and Xiong Y, 2001, Science 292: 1910-5. Medline: 11397945 Inoue T, Stuart J, Leno R, Maki CG, 2002, J. Biol. Chem. 277: 15053-60. Medline: 11847229 Sequence: 393 aa MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAP 80 TPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAM 160 AIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS 240 SCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKK 320 KPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ..................aaaaaaaaaMaMaa......................................... // NES-accession: NES-0003 Date: 14 Aug 2002 Protein: Ubiquitin-protein ligase E3 Mdm2 (p53-binding protein Mdm2) (Oncoprotein Mdm2) (Double minute 2 protein) (Hdm2) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q00987, MDM2_HUMAN Necessity: Mutation of L199A/I202A greatly impaires nuclear shuttling in a heterokaryon-assay Sufficiency: Aa 188-202 mediates export of GFP Aa 191-205 mediates export of GST Pathway: Rev-like pathway Location: Nucleus Regulation: - Comments: Discrepancy on Aa numbering: There is a difference of 6 in the numbering of amino acids between this sequence and these articles References: Roth J, Dobbelstein M, Freedman DA, Shenk T, Levine AJ, 1998 EMBO J 17: 554-64. Medline: 9430646 Zhang Y and Xiong Y, 2001 Science 292: 1910-5. Medline: 11397945 Sequence: 491 aa MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSND 80 LLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSS 160 RRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDS 240 VSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCN 320 RCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTS 400 SSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQ 480 PIQMIVLTYFP ................................................................................ 80 ................................................................................ 160 ...........................aaaaaaaaaaaMaaM...................................... 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ........... // NES-accession: NES-0004 Date: 14 Aug 2002 Protein: Xsmad4a Organism: Xenopus laevis (African clawed frog). DB_reference: TrEMBL Q9W639 Necessity: Necessary signal within the region of aa 134-320 (Deletional mutant context) Sufficiency: Aa 137-46 mediates export of ovalbumin Pathway: LMB-sensitive Location: Cytoplasmic Regulation: - Comments: - References: Watanabe M, Masuyama N, Fukuda M and Nishida E, 2000 EMBO Rep 1: 176-82. Medline: 11265759 Sequence: 549 aa MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQ 80 VAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSSAPSGLLVKDEYG 160 HDYAEGQQSISAGDGLSIQTIQHPPSSRSSTETYSNPALLVPSEPSTPNPTSFSSIPVPSTSQPASLLAATHNDGLLSIA 240 PVPPQGTQNGFSAQPATYHHNSTTTWTGSRTAAYTPNMSHHPNGHLQHHPPMPHPGHYWPVHNELAFQPPISNHPAPDYW 320 CSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLS 400 DHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAIS 480 LSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD ................................................................................ 80 ........................................................aaaaaaaaaa.............. 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ..................................................................... // NES-accession: NES-0005 Date: 14 Aug 2002 Protein: p120 catenin isoform 3AB Organism: Homo sapiens (Human) DB_reference: GenBank AF062338.1 Necessity: Exon B necessary for export Sufficiency: Aa 755-71 mediates export of ovalbumin. Pathway: LMB-sensitive Location: Isoforms containing exon B are exclusively cytoplasmic. Isoforms lacking exon B are equally distributed between nucleus and cytoplam Regulation: Regulation by alternative splicing. NES located in exon B. Comments: 32 different human isoforms References: van Hengel J, Vanhoenacker P, Staes K and van Roy F, 1999 Proc Natl Acad Sci U S A 96: 7980-5. Medline: 10393933 Sequence: 861 aa MQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRD 80 FRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQP 160 QVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQ 240 HERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPK 320 KEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEV 400 IIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVC 480 LLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRI 560 YISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELI 640 GKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQ 720 TIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTP 800 NERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ..................................aaaaaaaaaMaMaaaaa.......... // NES-accession: NES-0006 Date: 14 Aug 2002 Protein: MAP KINASE-ACTIVATED PROTEIN KINASE 2 (MAPK-ACTIVATED PROTEIN KINASE 2) (MAPKAP KINASE 2) (MAPKAPK-2) Organism: Mus musculus (Mouse) DB_reference: SWISS-PROT P49138, MKK2_MOUSE Necessity: A L345A mutation of the otherwise exclusively cytoplasmic T319E mutant, results in nuclear localization Sufficiency: Aa 330-353 is sufficient to mediate export of GFP, whereas neither aa 341-351 nor aa 337-353 are Pathway: LMB-sensitive Location: Predominantly nuclear Regulation: Phosphorylation of T319, but not T207, unmasks NES and allows export Comments: Atypical NES with weak similarity to EAIV and FIV Rev NESs. Discrepancy of 2 in amino acid numbering between article and retrieved sequence References: Engel K, Kotlyarov A and Gaestel M, 1998 EMBO J 17: 3363-71. Medline: 9628873 Sequence: 385 aa IRGSPGQTPPAPFPSPPPPAPAQPPPPFPQFHVKSGLQIRKNAITDDYKVTSQVLGLGINGKVLRIFDKRTQQKFALKML 80 QDCPKARREVELTWRASQCPHIVHIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQ 160 YLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYI 240 LLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTP 320 LHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARAVEDAALAH ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 .........aaaaaaaaaaaaaaaMaaaaaaaa................................ // NES-accession: NES-0007 Date: 14 Aug 2002 Protein: PROTO-ONCOGENE TYROSINE-PROTEIN KINASE ABL1 (P150) (C-ABL) Organism: Mus musculus (Mouse) DB_reference: SWISS-PROT P00520, ABL1_MOUSE Necessity: L1090A mutation results in exclusively nuclear localization of both full-length protein and aa 998-1079. Sufficiency: Aa 1078-96 mediates export of GST Pathway: LMB-sensitive Location: Predominantly nuclear Regulation: - Comments: Discrepancy of 26 in amino acid numbering between article and retrieved sequence.There is no sequence in the databases with the same aa numbering as the in the article References: Taagepera S, McDonald D, Loeb JE, Whitaker LL, McElroy AK, Wang JY and Hope TJ, 1998 Proc Natl Acad Sci U S A 95: 7457-62. Medline 9636171 Sequence: 1123 aa MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTL 80 SITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQ 160 RSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERT 240 DITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTY 320 GNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAK 400 FPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNP 480 SDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESD 560 ALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRE 640 LCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASC 720 MPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPLPGWLKKNEEAAEEGFKDT 800 ESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPG 880 RDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQS 960 TAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNS 1040 EQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDI 1120 VRR ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................................................ 880 ................................................................................ 960 ................................................................................ 1040 .....................................aaaaaaaaaaaaMaaaaaa........................ 1120 ... // NES-accession: NES-0008 Date: 14 Aug 2002 Protein: G2/MITOTIC-SPECIFIC CYCLIN B1 Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P14635, CGB1_HUMAN Necessity: V149A/L151A/V153A mutation abrogates export of full-length protein Sufficiency: Aa 141-52 mediates export of ovalbumin Pathway: LMB-sensitive Location: Cytoplasmic during S and G(2)-phases, but nuclear during prophase Regulation: - Comments: - References: Toyoshima F, Moriguchi T, Wada A, Fukuda M and Nishida E, 1998 EMBO J 17: 2728-35. Medline: 9582266 Sequence: 433 aa MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLP 80 KPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAED 160 GADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVP 240 KKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQH 320 TLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTV 400 KNKYATSKHAKISTLPQLNSALVQDLAKAVAKV ................................................................................ 80 ............................................................aaaaaaaaMaMaM....... 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................. // NES-accession: NES-0009 Date: 14 Aug 2002 Protein: Actin Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: SWISS-PROT P02579, ACT_YEAST Necessity: Mutation of both NESs (I178A/L180A/V219A/L221A), reults in exclusively nuclear localization. Mutation of one NES at the time results in inhibition of export Sufficiency: Both NESs individually sufficient to mediate export of ovalbumin. Shown with peptides of mammalian actin alfa corresponding to Sc actin aa 170-81 and aa 211-22 respectively Pathway: LMB-sensitive Location: Cytoplasmic Regulation: - Comments: 2 NESs in this sequence. References: Wada A, Fukuda M, Mishima M and Nishida E, 1998 EMBO J 17: 1635-41. Medline: 9501085 Sequence: 375 aa MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWD 80 DMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVT 160 HVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY 240 ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITAL 320 APSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF ................................................................................ 80 ................................................................................ 160 .........aaaaaaaaMaMa.............................aaaaaaaaMaMa.................. 240 ................................................................................ 320 ....................................................... // NES-accession: NES-0010 Date: 14 Aug 2002 Protein: HIV-1 Rev protein Organism: Human immunodeficiency virus type 1 (CDC-451 isolate) (HIV-1) DB_reference: SWISS-PROT P05865, REV_HV1C4. NLSdb NLS:166 Necessity: - Sufficiency: Aa 75-83 mediates export of GST and BSA. Export abrogated by the L78D/E79L and L83A mutations. Pathway: LMB-sensitive (Henderson and Eleftheriou 2000). Location: - Regulation: - Comments: - References: Fischer U, Huber J, Boelens WC, Mattaj IW and Luhrmann R, 1995 Cell 82: 475-83. Medline: 7543368 Henderson BR and Eleftheriou A, 2000 Exp Cell Res 2000 256: 213-24, Medline: 10739668 Sequence: 116 aa MAGRSGDSDEELIKTVRIIKHLYQSNPPPKPEGTRQARRNRRRRWRERQRRIHSISAWLLSTCLGRSAEPVPLQLPPLER 80 LTLDCSEDCGTSGTQGVGSPQILVESPAVLESGTKE ..........................................................................aaaMMa 80 aaM................................. // NES-accession: NES-0011 Date: 14 Aug 2002 Protein: Rex protein Organism: Human T-lymphotropic virus 1 DB_reference: TrEMBL Q82233 Necessity: - Sufficiency: Aa 81-94 mediates export of GST. Export abrogated by L90A/L92A. Pathway: - Location: - Regulation: - Comments: - References: Bogerd HP, Fridell RA, Benson RE, Hua J and Cullen BR, 1996 Mol Cell Biol 16: 4207-14. Medline: 8754820 Sequence: 189 aa MPKTRRRPRRSQRKRPPTPWPTSQGLDRVFFSDTQSTCLETVYKATGAPSLGDYVRPAYIVTPYWPPVQSIRSPGTPSMD 80 ALSAQLYSSLSLDSPPSPPREPLRPSRSLPRQSLIQPPTFHPPSSRPCANTPPSEMDTWNPPLGSTSQPCLFQTPDSGPK 160 TCTPSGEAPLSACTSTSFPPPSPGPSCPT ...............................................................................a 80 aLaaaLaaaMaMaa.................................................................. 160 ............................. // NES-accession: NES-0012 Date: 14 Aug 2002 Protein: Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1) Organism: Xenopus laevis (African clawed frog) DB_reference: SWISS-PROT Q05116, MPK1_XENLA Necessity: Delta 31-50, L32A/L36A and L39A/L41A all lead to whole cell distribution Sufficiency: Aa 32-44 mediates export of ovalbumin, which is abrogated by L32A/L36A/L39A/L41A Pathway: LMB-sensitive Location: Cytoplasmic Regulation: - Comments: Discrepancy of 1 in amino acid numbering between article and retrieved sequence References: Fukuda M, Gotoh I, Gotoh Y and Nishida E, 1996 J Biol Chem 271: 20024-8. Medline: 8702720 Fukuda M, Asano S, Nakamura T, Adachi M, Yoshida M, Yanagida M and Nishida E, 1997 Nature 390: 308-11. Medline: 9384386 Sequence: 394 aa PKKKPTPIQLNPNPEGTAVNGTPTAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKVSELGAGNGGV 80 VFKVSHKPTSLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGKI 160 PEKILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYS 240 VQSDIWSMGLSLVEMAIGRYPIPPPDAKELELIFGCSVERDPASSELAPRPRPPGRPISSYGPDSRPPMAIFELLDYIVN 320 EPPPKLPSGVFGAEFQDFVNKCLVKNPAERADLKQLMVHSFIKQSELEEVDFAGWLCSTMGLKQPSTPTHAAGV ...............................MaaaMaaMaMaaa.................................... 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 .......................................................................... // NES-accession: NES-0013 Date: 14 Aug 2002 Protein: EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15 (PROTEIN EPS15) (AF-1P PROTEIN) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P42566, EP15_HUMAN Necessity: Aa 766-896 necessary in full-length context. Aa 849-896 necessary in a deletional mutant context. I893A, L882A/L884A and I886A/L888A leads to nucelar accumulation. Aa 890-96 indirectly necessary, since aa 766-896 is sufficient to mediate export of GFP, whereas aa 766-890 is not Sufficiency: Aa 766-896 is mediates export of GFP, whereas aa 766-890 does not. Pathway: LMB-sensitive Location: Cytoplasmic Regulation: - Comments: - References: Poupon V, Polo S, Vecchi M, Martin G, Dautry-Varsat A, Cerf-Bensussan N, Di Fiore PP and Benmerah A, 2002 J Biol Chem 277: 8941-8. Medline: 11777906 Sequence: 896 aa MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNKQEFFVALRLV 80 ACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKL 160 PVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMD 240 GFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNI 320 IGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDE 400 LDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQ 480 HLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPA 560 RSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKID 640 PFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESF 720 GGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPF 800 KLNDPFQPFPGNDSPKEKDPEMFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQE 880 DLELAIALSKSEISEA ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 .............................................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 800 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 880 aMaMaMaMaaaaMaaa // NES-accession: NES-0014 Date: 14 Aug 2002 Protein: Breast cancer type 1 susceptibility protein (BRCA1) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P38398, BRC1_HUMAN. NLSdb NLS:49 Necessity: L86A/I90A abrogates export of full-length protein Sufficiency: Aa 81-99 mediates export of GFP Pathway: LMB-sensitive and Crm-dependent Location: Whole cell distribution in most cells, but also exclusively either nuclear or cytoplasmic in some cells Regulation: - Comments: - References: Rodriguez JA, Henderson BR, 2000 J Biol Chem 275: 38589-96. Medline: 10991937 Sequence: 1863 aa MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFS 80 QLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLG 160 TVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQ 240 PSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNR 320 WAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHD 400 GESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTEN 480 LIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGD 560 SIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQ 640 IDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKE 720 FVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNK 800 CVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEE 880 ECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRG 960 NETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEAS 1040 SSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTV 1120 NTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQ 1200 GYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKAS 1280 QEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQS 1360 MDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALE 1440 DLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNR 1520 NYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSAL 1600 KVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLI 1680 TEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKI 1760 FRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLD 1840 SVALYQCQELDTYLIPQIPHSHY ................................................................................ 80 aaaaaMaaaMaaaaaaaaa............................................................. 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................................................ 880 ................................................................................ 960 ................................................................................ 1040 ................................................................................ 1120 ................................................................................ 1200 ................................................................................ 1280 ................................................................................ 1360 ................................................................................ 1440 ................................................................................ 1520 ................................................................................ 1600 ................................................................................ 1680 ................................................................................ 1760 ................................................................................ 1840 ....................... // NES-accession: NES-0015 Date: 14 Aug 2002 Protein: Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (RelA) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q04206, TF65_HUMAN. NLSdb NLS:17 Necessity: Delta 440-43 abrogates export of full-length protein Sufficiency: Aa 436-45 is mediates export of a p50-GFP fusion construct Pathway: LMB-sensitive Location: Cytoplasmic when not complexed with p50 subunit of NF-kappa-B Regulation: - Comments: - References: Harhaj EW and Sun SC, 1999 Mol Cell Biol 19: 7088-95. Medline: 10490645 Sequence: 551 aa MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKD 80 PPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLC 160 FQVTVRDPSGRPLRLPPVLPHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFS 240 QADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSG 320 PTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMV 400 SALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQ 480 GIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ...................................aaaaMMMMaa................................... 480 ....................................................................... // NES-accession: NES-0016 Date: 14 Aug 2002 Protein: Heat shock factor protein HSF30 (Heat shock transcription factor 30) (HSTF 30) (Heat stress transcription factor) (HsfA2) Organism: Lycopersicon peruvianum (Peruvian tomato) DB_reference: SWISS-PROT P41152, HSF3_LYCPE Necessity: Deletion of the most C-terminal 8 residues or mutation of L344A/L348A/L351A abrogates export Sufficiency: C-terminal 23 aa mediates export of the otherwise completely nuclear HsfB1. Pathway: LMB-sensitive Location: Cytosolic Regulation: Becomes nuclear upon coexpression of HsfA1 Comments: - References: Heerklotz D, Doring P, Bonzelius F, Winkelhaus S and Nover L, 2001 Mol Cell Biol 21: 1759-68. Medline: 11238913 Sequence: 351 aa MEDVMKVKVEEDGIPTAVLPMEGLHDVGPPPFLSKTYEMVEDSSTDQVISWSTTRNSFIVWDSHKFSTTLLPRFFKHSNF 80 SSFIRQLNTYGFRKVDPDRWEFANEGFLGGQKHLLKTIKRRRNVGQSMNQQGSGACIEIGYYGMEEELERLKRDKNVLMT 160 EIVKLRQQQQSTRNQIIAMGEKIETQERKQVQMMSFLAKIFSNPTFLQQYLDKQVHRKDKQRIEVGQKRRLTMTPSVTGS 240 DQPMNYSSSLQESEAELASIEMLFSAAMDNESSSNVRPDSVVTANGTDMEPVADDIWEELLSEDLISGDRAAEEVVVVEQ 320 PEFDVEVEDLVVKTPEWGEELQDLVDQLGFL ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 .......aaaaaaaaaaaaaaaaMaaaMaaM // NES-accession: NES-0017 Date: 14 Aug 2002 Protein: AP-1-like transcription factor (Pap1) Organism: Schizosaccharomyces pombe (Fission yeast) DB_reference: SWISS-PROT Q01663, AP1_SCHPO Necessity: Delta 488-544 abrogates export Sufficiency: Aa 515-533 mediates export of GST-GFP fusion. Export abrogated by L522A, L526A, F517A/I519A and C523S/C532S and greatly impaired by I519A and C532S Pathway: Crm-dependent, LMB-sensitive Location: Cytoplasmic Regulation: Regulated by oxidation of cysteine in the NES region Comments: - References: Kudo N, Taoka H, Toda T, Yoshida M and Horinouchi S, 1999 J Biol Chem 274: 15151-8. Medline: 10329722 Sequence: 552 aa MSGQTETLSSTSNIPIAKAEPEQSADFSASHKKRGPVSDRSSRRTSSEEVDLMPNVDDEVDGDVKPKKIGRKNSDQEPSS 80 KRKAQNRAAQRAFRKRKEDHLKALETQVVTLKELHSSTTLENDQLRQKVRQLEEELRILKDGSFTFEMSLPHRNPSLSSL 160 PTTGFSSNFAHMKDGISPQSNLHLSPNSIEKPNMHQNVLHNDRSADNLNHRYQVPPTLVDSNSAQGTLSPETPSSSDSPS 240 NLYLNYPKRKSITHLHHDCSALSNGENGEDVADGKQFCQKLSTACGSIACSMLTKTTPHRASVDILSNLHESTVSPPMAD 320 ESVQRSSEVSKSIPNVELSLNVNQQFVSPFGGTDSFPLPTDTGLDSLFEPDSAIENSHLKNVVMEPELFQAWREPAESLD 400 KEFFNDEGEIDDVFHNYFHNSNENGDLITNSLHGLDFLENANESFPEQMYPFIKHNKDYISNHPDEVPPDGLPQKGKHDT 480 SSQMPSENEIVPAKERAYLSCPKVWSKIINHPRFESFDIDDLCSKLKNKAKCSSSGVLLDERDVEAALNQFN ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ..................................aaMaMaaMMaaMaaaaaMa................... // NES-accession: NES-0018 Date: 14 Aug 2002 Protein: Signal transducer and activator of transcription 1-alpha/beta (Transcription factor ISGF-3 components p91/p84) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P42224, STA1_HUMAN Necessity: L199A greatly impairs export Sufficiency: Aa 197-205 sufficient to mediate export of GFP Pathway: LMB-sensitivity, Crm-interaction Location: - Regulation: - Comments: - References: Mowen K and David M, 2000 Mol Cell Biol 20: 7273-81. Medline: 10982844 Sequence: 750 aa MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQ 80 HNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEI 160 KSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWK 240 RRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVE 320 RQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSL 400 AAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAE 480 PRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIES 560 ILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELS 640 AVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTT 720 DNLLPMSPEEFDEVSRIVGSVEFDSMMNTV ................................................................................ 80 ................................................................................ 160 ....................................aaMaaaaaa................................... 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 .............................. // NES-accession: NES-0019 Date: 14 Aug 2002 Protein: Metal-regulatory transcription factor (MTF1) Organism: Homo sapiens (Human) DB_reference: TrEMBL Q14872 Necessity: L338A/L341A/L343A/L344A abrogates export of full-length MTF1 Sufficiency: Aa 336-44 mediates nuclear exclusion of tandem eGFP Pathway: LMB-sensitive Location: - Regulation: - Comments: - References: Saydam N, Georgiev O, Nakano MY, Greber UF and Schaffner W, 2001 J Biol Chem 276: 25487-95. Medline: 11306562 Sequence: 753 aa MGEHSPDNNIIYFEAEEDELTPDDKMLRFVDKNGLVPSSSGTVYDRTTVLIEQDPGTLEDEDDDGQCGEHLPFLVGGEEG 80 FHLIDHEAMSQGYVQHIISPDQIHLTINPGSTPMPRNIEGATLTLQSECPETKRKEVKRYQCTFEGCPRTYSTAGNLRTH 160 QKTHRGEYTFVCNQEGCGKAFLTSHSLRIHVRVHTKEKPFECDVQGCEKAFNTLYRLKAHQRLHTGKTFNCESEGCSKYF 240 TTLSDLRKHIRTHTGEKPFRCDHDGCGKAFAASHHLKTHVRTHTGERPFFCPSNGCEKTFSTQYSLKSHMKGHDNKGHSY 320 NALPQHNGSEDTNHSLCLSDLSLLSTDSELRENSSTTQGQDLSTISPAIIFESMFQNSDDTAIQEDPQQTASLTESFNGD 400 AESVSDVPPSTGNSASLSLPLVLQPGLSEPPQPLLPASAPSAPPPAPSLGPGSQQAAFGNPPALLQPPEVPVPHSTQFAA 480 NHQEFLPHPQAPQPIVPGLSVVAGASASAAAVASAVAAPAPPQSTTEPLPAMVQTLPLGANSVLTNNPTITITPTPNTAI 560 LQSSLVMGEQNLQWILNGATSSPQNQEQIQQASKVEKVFFTTAVPVASSPGSSVQQIGLSVPVIIIKQEEACQCQCACRD 640 SAKERASSRRKGCSSPPPPEPSPQAPDGPSLQLPAQTFSSAPVPGSSSSTLPSSCEQSRQAETPSDPQTETLSAMDVSEF 720 LSLQSLDTPSNLIPIEALLQGEEEMGLTSSFSK ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ...............aaMaaMaMM........................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................. // NES-accession: NES-0020 Date: 14 Aug 2002 Protein: Heat shock protein SSB1 (Cold-inducible protein YG101) (Ssb1) Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: SWISS-PROT P11484, HS75_YEAST Necessity: Delta 546-613 in full-length context leads to whole cell distribution Sufficiency: Aa 546-99 mediates complete export of GFP-protein A fusion. Export mediated by aa 546-86 is less efficient. Delta 574-87 in a 545-612 context abrogates export Pathway: Crm-dependent Location: Cytoplasmic Regulation: - Comments: - References: Shulga N, James P, Craig EA and Goldfarb DS, 1999 J Biol Chem 274: 16501-7. Medline: 10347213 Sequence: 612 aa AEGVFQGAIGIDLGTTYSCVATYESSVEIIANEQGNRVTPSFVAFTPEERLIGDAAKNQAALNPRNTVFDAKRLIGRRFD 80 DESVQKDMKTWPFKVIDVDGNPVIEVQYLEETKTFSPQEISAMVLTKMKEIAEAKIGKKVEKAVITVPAYFNDAQRQATK 160 DAGAISGLNVLRIINEPTAAAIAYGLGAGKSEKERHVLIFDLGGGTFDVSLLHIAGGVYTVKSTSGNTHLGGQDFDTNLL 240 EHFKAEFKKKTGLDISDDARALRRLRTAAERAKRTLSSVTQTTVEVDSLFDGEDFESSLTRARFEDLNAALFKSTLEPVE 320 QVLKDAKISKSQIDEVVLVGGSTRIPKVQKLLSDFFDGKQLEKSINPDEAVAYGAAVQGAILTGQSTSDETKDLLLLDVA 400 PLSLGVGMQGDMFGIVVPRNTTVPTIKRRTFTTCADNQTTVQFPVYQGERVNCKENTLLGEFDLKNIPMMPAGEPVLEAI 480 FEVDANGILKVTAVEKSTGKSSNITISNAVGRLSSEEIEKMVNQAEEFKAADEAFAKKHEARQRLESYVASIEQTVTDPV 560 LSSKLKRGSKSKIEAALSDALAALQIEDPSADELRKAEVGLKRVVTKAMSSR ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 .............aaaaaaaaaaaaaa......................... // NES-accession: NES-0021 Date: 14 Aug 2002 Protein: RNA export factor GLE1 Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: SWISS-PROT Q12315, GLE1_YEAST. NLSdb NLS:270 Necessity: Delta 351-58 is inviable. Each of the mutantions L351A, L353A, L356A, and L358A lead to inhibition of poly(a)+ RNA export Sufficiency: Aa 350-58 mediates export of HSA (human serum albumin). Each of the above-mentioned mutants abrogates export Pathway: - Location: Predominantly at nuclear pore complexes Regulation: - Comments: - References: Murphy R and Wente SR, 1996 Nature 383: 357-60. Medline: 8848052 Sequence: 538 aa MRFVFDEVFNSDTDSPEFEETCSTTSSTSSQCPTPEPSPAIKLPSFTKVGTKKLVNESVVILDPALENALRDLNLQSKLI 80 PINEPIVAASSIIVPHSTNMPLPRASHSSLLDNAKNSNATAPLLEAIEESFQRKMQNLVLANQKEIQSIRENKRRVEEQR 160 KRKEEEERKRKEAEEKAKREQELLRQKKDEEERKRKEAEAKLAQQKQEEERKKIEEQNEKERQLKKEHEAKLLQQKDKLG 240 KAVTNFDKISKMFWHYKDKIAQIKQDIVLPIKKADVNVRNLLSRHKRKINPKFGQLTNSNQQLFKIQNELTQLINDTKGD 320 SLAYHWILNFIAKAVVHQAETEVRVKPESALPLGKLTLYLLVQFPELQELFMARLVKKCPFVIGFTCEIDTEKGRQNMGW 400 KRNNENKWEDNTSYDERMGGILSLFAIITRLQLPQEFITTTSHPFPIALSWHILARICNTPLNLITNTHFVILGSWWDAA 480 AVQFLQAYGNQASKLLILIGEELTSRMAEKKYVGAARLRILLEAWQNNNMESFPEMSP ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 .............................aMaMaaMaM.......................................... 400 ................................................................................ 480 .......................................................... // NES-accession: NES-0022 Date: 14 Aug 2002 Protein: G2/mitotic-specific cyclin B1 Organism: Xenopus laevis (African clawed frog) DB_reference: SWISS-PROT P13350, CGB1_XENLA Necessity: F112A in full-length context severely impaires export. Sufficiency: Aa 76-125 mediates export of GST. Pathway: Competition by PKI-NES, Crm-interaction, LMB-sensitivity. Location: Cytoplasmic during interphase. Regulation: Possibly by phosphorylation. Comments: - References: Yang J, Bardes ES, Moore JD, Brennan J, Powers MA and Kornbluth S, 1998 Genes Dev 12: 2131-43. Medline: 9679058 Sequence: 397 aa MSLRVTRNMLANAENNVKTTLAGKRVVATKPGLRPRTALGDIGNKAEVKVPTKKELKPAVKAAKKAKPVDKLLEPLKVIE 80 ENVCPKPAQVEPSSPSPMETSGCLPDELCQAFSDVLIHVKDVDADDDGNPMLCSEYVKDIYAYLRSLEDAQAVRQNYLHG 160 QEVTGNMRAILIDWLVQVQMKFRLLQETMFMTVGIIDRFLQEHPVPKNQLQLVGVTAMFLAAKYEEMYPPEIGDFTFVTD 240 HTYTKAQIRDMEMKILRVLKFAIGRPLPLHFLRRASKIGEVTAEQHSLAKYLMELVMVDYDMVHFTPSQIAAASSCLSLK 320 ILNAGDWTPTLHHYMAYSEEDLVPVMQHMAKNIIKVNKGLTKHLTVKNKYASSKQMKISTIPQLRSDVVVEMARPLM ...........................................................................aaaaa 80 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaaaaaaaaaaaa................................... 160 ................................................................................ 240 ................................................................................ 320 ............................................................................. // NES-accession: NES-0023 Date: 14 Aug 2002 Protein: Period circadian protein 1 (Circadian pacemaker protein Rigui) (mPER) (M-Rigui) Organism: Mus musculus (Mouse) DB_reference: SWISS-PROT O35973, PER1_MOUSE Necessity: I493A/L496A abrogates export of full-length PER1. Sufficiency: Delta 1-484 is excluded from the nucleus, whereas delta 1-495 has a hole cell distribution. Aa 485-98 mediates export of YFP(2)-NLS, but export is abrogated by I493A/L496A/L498K. Pathway: LMB-sensitive Location: - Regulation: Phosphorylation by casein kinase I epsilon masks NLS and results in cytoplasmic accumulation.< Comments: - References: Vielhaber EL, Duricka D, Ullman KS and Virshup DM, 2001 J Biol Chem 276: 45921-7. Medline: 11591712 Sequence: 1291 aa MSGPLEGADGGGDPRPGEPFCPGGVPSPGAPQHRPCPGPSLADDTDANSNGSSGNESNGPESRGASQRSSHSSSSGNGKD 80 SALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMTALRELKLRLPPERRGKGRSG 160 TLATLQYALACVKQVQANQEYYQQWSLEEGEPCAMDMSTYTLEELEHITSEYTLRNQDTFSVAVSFLTGRIVYISEQAGV 240 LLRCKRDVFRGARFSELLAPQDVGVFYGSTTPSRLPTWGTGTSAGSGLKDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTP 320 YVTKIRVSDGAPAQPCCLLIAERIHSGYEAPRIPPDKRIFTTRHTPSCLFQDVDERAAPLLGYLPQDLLGAPVLLFLHPE 400 DRPLMLAIHKKILQLAGQPFDHSPIRFCARNGEYVTMDTSWAGFVHPWSRKVAFVLGRHKVRTAPLNEDVFTPPAPSPAP 480 SLDSDIQELSEQIHRLLLQPVHSSSPTGLCGVGPLMSPGPLHSPGSSSDSNGGDAEGPGPPAPVTFQQICKDVHLVKHQG 560 QQLFIESRAKPPPRPRLLATGTFKAKVLPCQSPNPELEVAPVPDQASLALAPEEPERKETSGCSYQQINCLDSILRYLES 640 CNIPSTTKRKCASSSSYTASSASDDDKQRAGPVPVGAKKDPSSAMLSGEGATPRKEPVVGGTLSPLALANKAESVVSVTS 720 QCSFSSTIVHVGDKKPPESDIIMMEDLPGLAPGPAPSPAPSPTVAPDPTPDAYRPVGLTKAVLSLHTQKEEQAFLNRFRD 800 LGRLRGLDTSSVAPSAPGCHHGPIPPGRRHHCRSKAKRSRHHHHQTPRPETPCYVSHPSPVPSSGPWPPPPATTPFPAMV 880 QPYPLPVFSPRGGPQPLPPAPTSVSPATFPSPLVTPMVALVLPNYLFPTPPSYPYGVSQAPVEGPPTPASHSPSPSLPPP 960 PLSPPHRPDSPLFNSRCSSPLQLNLLQLEESPRTEGGAAAGGPGSSAGPLPPSEETAEPEARLVEVTESSNQDALSGSSD 1040 LLELLLQEDSRSGTGSAASGSLGSGLGSGSGSGSHEGGSTSASITRSSQSSHTSKYFGSIDSSEAEAGAARARTEPGDQV 1120 IKCVLQDPIWLLMANADQRVMMTYQVPSRDAASVLKQDRERLRAMQKQQPRFSEDQRRELGAVHSWVRKGQLPRALDVMA 1200 CVDCGSSVQDPGHSDDPLFSELDGLGLEPMEEGGGEGGGCGVGGGGGDGGEEAQTQIGAKGSSSQDSAMEEEEQGGGSSS 1280 PALPAEENSTS ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ....aaaaaaaaMaaMaM.............................................................. 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................................................ 880 ................................................................................ 960 ................................................................................ 1040 ................................................................................ 1120 ................................................................................ 1200 ................................................................................ 1280 ........... // NES-accession: NES-0024 Date: 14 Aug 2002 Protein: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta 1 (PLC-delta-1) (Phospholipase C-delta-1) (PLC-III) Organism: Rattus norvegicus (Rat) DB_reference: SWISS-PROT P10688, PID1_RAT Necessity: L172A/I174A greatly impairs export of full-length protein. Sufficiency: Aa 164-77 mediates export of ovalbumin, whereas the mutant peptide L165A/L169A/L172A/LI174A does not. Pathway: LMB-sensitive. Location: Predominantly at the plasma membrane. Regulation: Proposal of calciumregulated mechanism based on structural comparison with EF-hand structure. Comments: NES located in EF-hand. References: Yamaga M, Fujii M, Kamata H, Hirata H and Yagisawa H, 1999 J Biol Chem 274: 28537-41. Medline: 10497218 Sequence: 756 aa MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHR 80 TEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVQGLRKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKM 160 NFKELKDFLKELNIQVDDGYARKIFRECDHSQTDSLEDEEIETFYKMLTQRAEIDRAFEEAAGSAETLSVERLVTFLQHQ 240 QREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMDQPLSHYLVSSSHNTYLLEDQL 320 TGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRV 400 MARHLRAILGPILLDQPLDGVTTSLPSPEQLKGKILLKGKKLGGLLPAGGENGSEATDVSDEVEAAEMEDEAVRSQVQHK 480 PKEDKLKLVPELSDMIIYCKSVHFGGFSSPGTSGQAFYEMASFSESRALRLLQESGNGFVRHNVSCLSRIYPAGWRTDSS 560 NYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPNTTFNSRALTQGPWWRPERLRVRIISGQQ 640 LPKVNKNKNSIVDPKVIVEIHGVGRDTGSRQTAVITNNGFNPRWDMEFEFEVTVPDLALVRFMVEDYDSSSKNDFIGQST 720 IPWNSLKQGYRHVHLLSKNGDQHPSATLFVKISIQD ................................................................................ 80 ................................................................................ 160 ...aMaaaMaaMaMaaa............................................................... 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 .................................... // NES-accession: NES-0025 Date: 14 Aug 2002 Protein: E1B protein, large T-antigen (E1B-55K) Organism: Human adenovirus type 5 DB_reference: SWISS-PROT P03243, E1BL_ADE05 Necessity: L83A, L87A and L91A abrogates export of full-length protein. Sufficiency: Aa 83-93 mediates export of GST-GFP fusion. Pathway: LMB-sensitive. Location: Cytoplasmic. Regulation: - Comments: - References: Kratzer F, Rosorius O, Heger P, Hirschmann N, Dobner T, Hauber J and Stauber RH, 2000, Oncogene 19: 850-7. Medline: 10702793 Sequence: 496 aa MERRNPSERGVPAGFSGHASVESGCETQESPATVVFRPPGDNTDGGAAAAAGGSQAAAAGAEPMEPESRPGPSGMNVVQV 80 AELYPELRRILTITEDGQGLKGVKRERGACEATEEARNLAFSLMTRHRPECITFQQIKDNCANELDLLAQKYSIEQLTTY 160 WLQPGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAEVEIDTEDRVAFRCSMINMWPGVLGMDGVVIMNVR 240 FTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWTDVRVRGCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNS 320 RVRHNVASDCGCFMLVKSVAVIKHNMVCGNCEDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGNR 400 RGVFLPYQCNLSHTKILLEPESMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGGKHIRNQPVMLDVTEELRPDHLV 480 LACTRAEFGSSDEDTD ................................................................................ 80 ..MaaaMaaaMaa................................................................... 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................ // NES-accession: NES-0026 Date: 14 Aug 2002 Protein: 38 kDa MOV34 ISOLOGUE (Kip1 C-terminus interacting protein-2) (Jab1/CSN5) Organism: Mus musculus (Mouse) DB_reference: TrEMBL O35864 Necessity: L237A/L240A/L241A abrogates export. Sufficiency: Aa 233-242 is suficient to interact with Crm in vitro, the interaction being disrupted by L237A/L240A/L241A. Pathway: LMB-sensitive, Crm-interaction. Location: Whole cell distribution. Regulation: - Comments: - References: Tomoda K, Kubota Y, Arata Y, Mori S, Maeda M, Tanaka T, Yoshida M, Yoneda-Kato N and Kato JY, 2002 J Biol Chem 277: 2302-10. Medline: 11704659 Sequence: 334 aa MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGL 80 MLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQ 160 FQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLEL 240 LWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMS 320 QVIKDKLFNQINVA ................................................................................ 80 ................................................................................ 160 ........................................................................aaaaMaaM 240 Ma.............................................................................. 320 .............. // NES-accession: NES-0027 Date: 14 Aug 2002 Protein: Tristetraproline (TTP) (TIS11A protein) (TIS11) (ZFP-36) Organism: Rattus norvegicus (Rat) DB_reference: SWISS-PROT P47973, TTP_RAT Necessity: Delta 1-15 and L3A/I6A/L10A both abrogate export of aa 1-161. Sufficiency: Aa 1-101 mediates export of GFP. Pathway: LMB sensitive. Location: Whole cell distribution. Regulation: - Comments: - References: Murata T, Yoshino Y, Morita N and Kaneda N, 2002 Biochem Biophys Res Commun 293: 1242-7. Medline: 12054509 Sequence: 320 aa MDLSAIYESLMSMSHDLSPDHGGTESSGGLWNINSSDSIPSGVTSRLTGRSTSLVEGRSCSWVPPPPGFAPLAPRPGPEL 80 SPSPTSPTATPTTSSRYKTELCRTYSESGRCRYGAKCQFAHGPGELRQANRHPKYKTELCHKFYLQGRCPYGSRCHFIHN 160 PTEDLALPGQPHVLRQSISFSGLPSGRRTSPPPPGFSGPSLSSCSFSPSSSPPPPGDLPLSPSAFSAAPGTPVSRRDPTP 240 ACCPSCRRSTTPSTIWGPLGGLARSPSAHSLGSDPDDYASSGSSLGGSDSPVFEAGVFGPPQPPAPPRRLPIFNRISVSE aaMaaMaaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 80 aaaaaaaaaaaaaaaaaaaaa........................................................... 160 ................................................................................ 240 ................................................................................ // NES-accession: NES-0028 Date: 14 Aug 2002 Protein: NF-kappaB inhibitor alpha (Major histocompatibility complex enhancer- binding protein MAD3) (I-kappa-B-alpha) (IkappaBalpha) (IKB-alpha) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P25963, IKBA_HUMAN Necessity: I52A/L54A abrogates export of full-length I-kappa-B-alpha fused to GST. Sufficiency: Aa 22-72 restores rev-delta-NES acitivity (export). Pathway: LMB-sensitive. Location: Cytoplasmic. Regulation: - Comments: - References: Johnson C, Van Antwerp D and Hope TJ, 1999 EMBO J 18: 6682-93. Medline: 10581242 Sequence: 317 aa MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHL 80 AIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASV 160 GVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCG 240 ADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL .....................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaMaaaaaaaaaaaaaaaaaa........ 80 ................................................................................ 160 ................................................................................ 240 ............................................................................. // NES-accession: NES-0029 Date: 14 Aug 2002 Protein: Ran-specific GTPase-activating protein (Ran binding protein 1) (RanBP1) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P43487, RANG_HUMAN Necessity: L184A/L187A/V189A abrogates export of full-length RanBP1-GFP Sufficiency: Aa 178-95 can substitute Rev-NES Pathway: Rev competition (same pathway). Plafker et al. 2000 has furthermore shown LMB-sensitivity. Location: Cytoplasmic. Regulation: - Comments: Discrepancy of 1 in amino acid numbering between article and retrieved sequence. References: Zolotukhin AS and Felber BK, 1997 J Biol Chem 1997 272: 11356-60. Medline: 9111043 Plafker K, Macara IG, 2000 Mol Cell Biol 20: 3510-21. Medline: 10779340 Sequence: 201 aa MAAAKDTHEDHDTSTENTDESNHDPQFEPIVSLPEQEIKTLEEDEEELFKMRAKLFRFASENDLPEWKERGTGDVKLLKH 80 KEKGAIRLLMRRDKTLKICANHYITPMMELKPNAGSDRAWVWNTHADFADECPKPELLAIRFLNAENAQKFKTKFEECRK 160 EIEEREKKAGSGKNDHAEKVAEKLEALSVKEETKEDAEEKQ ................................................................................ 80 ................................................................................ 160 .................aaaaaaMaaMaMaaaaaa...... // NES-accession: NES-0030 Date: 14 Aug 2002 Protein: Interferon regulatory factor 3 (IRF-3) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q14653, IRF3_HUMAN Necessity: I139A/L140A/L143A/L144A abrogates export of full-length IRF-3. Sufficiency: Aa 136-150 mediates export of ovalbumin. Export abrogated by I139A/L140A/L143A/L144A/M147A/V148A/L149A. Pathway: LMB-sensitive (data not shown). Location: Cytoplasmic. Regulation: Nuclear upon infection with nexcastle disease virus (NDV). Comments: - References: Yoneyama M, Suhara W, Fukuhara Y, Fukuda M, Nishida E and Fujita T, 1998 EMBO J 17: 1087-95. Medline: 9463386 Sequence: 427 aa MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNF 80 RSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSL 160 AVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPG 240 WPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVF 320 DLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHP 400 LSLTSDQYKAYLQDLVEGMDFQGPGES ................................................................................ 80 .......................................................aaaMMaaMMaaaaaa.......... 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ........................... // NES-accession: NES-0031 Date: 14 Aug 2002 Protein: Tumor protein p73 (p53-like transcription factor) (p53-related protein) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT O15350, P73_HUMAN. NLSdb NLS:270 Necessity: L375A/L377A abrogates export of full-length p73. Sufficiency: Aa 364-82 mediates export of YFP(2). Export abrogated by abovementioned mutations. Pathway: LMB-sensitive. Location: Nuclear. Regulation: - Comments: - References: Inoue T, Stuart J, Leno R and Maki CG, 2002 J Biol Chem 277: 15053-60. Medline: 11847229 Sequence: 636 aa MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAA 80 SASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCP 160 IQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPY 240 EPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSA 320 KNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPS 400 HLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSH 480 CTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQL 560 LRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ...........................................aaaaaaaaaaaMaMaaaaa.................. 400 ................................................................................ 480 ................................................................................ 560 ............................................................................ // NES-accession: NES-0032 Date: 14 Aug 2002 Protein: MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 1 (SMAD 1) (MOTHERS AGAINST DPP HOMOLOG 1) (MAD-RELATED PROTEIN 1) (TRANSFORMING GROWTH FACTOR- BETA SIGNALING PROTEIN-1) (BSP-1) (HSMAD1) (JV4-1) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q15797, SMA1_HUMAN Necessity: L406A/M409A abrogates export. Sufficiency: Aa 406-14 mediates export of GST. Export is abrogated by L406A/M409A. Pathway: LMB-sensitivity, Crm-dependency. Location: Whole cell distribution. Regulation: - Comments: - References: Xiao Z, Watson N, Rodriguez C and Lodish HF, 2001 J Biol Chem 276: 39404-10. Medline: 11509558 Sequence: 465 aa MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHR 80 KGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLG 160 QNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ 240 PMDTNMMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRN 320 STIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGF 400 ETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 .....MaaMaaaaa................................................... // NES-accession: NES-0033 Date: 14 Aug 2002 Protein: DUAL SPECIFICITY PROTEIN PHOSPHATASE 1 (MITOGEN-ACTIVATED PROTEIN KINASE PHOSPHATASE 7) (MAP KINASE PHOSPHATASE 7) (MKP-7) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q9BY84, DUSX_HUMAN Necessity: L380A/L383A/L385A abrogates export. Sufficiency: - Pathway: LMB-sensitive. Location: Cytoplasmic. Regulation: - Comments: - References: Masuda K, Shima H, Watanabe M and Kikuchi K, 2001, J Biol Chem 276: 39002-11. Medline: 11489891 Sequence: 665 aa MAHEMIGTQIVTERLVALLESGTEKVLLIDSRPFVEYNTSHILEAININCSKLMKRRLQQDKVLITELIQHSAKHKVDID 80 CSQKVVVYDQSSQDVASLSSDCFLTVLLGKLEKSFNSVHLLAGGFAEFSRCFPGLCEGKSTLVPTCISQPCLPVANIGPT 160 RILPNLYLGCQRDVLNKELMQQNGIGYVLNASNTCPKPDFIPESHFLRVPVNDSFCEKILPWLDKSVDFIEKAKASNGCV 240 LVHCLAGISRSATIAIAYIMKRMDMSLDEAYRFVKEKRPTISPNFNFLGQLLDYEKKIKNQTGASGPKSKLKLLHLEKPN 320 EPVPAVSEGGQKSETPLSPPCADSATSEAAGQRPVHPASVPSVPSVQPSLLEDSPLVQALSGLHLSADRLEDSNKLKRSF 400 SLDIKSVSYSASMAASLHGFSSSEDALEYYKPSTTLDGTNKLCQFSPVQELSEQTPETSPDKEEASIPKKLQTARPSDSQ 480 SKRLHSVRTSSSGTAQRSLLSPLHRSGSVEDNYHTSFLFGLSTSQQHLTKSAGLGLKGWHSDILAPQTSTPSLTSSWYFA 560 TESSHFYSASAIYGGSASYSAYSCSQLPTCGDQVYSVRRRQKPSDRADSRRSWHEESPFEKQFKRRSCQMEFGESIMSEN 640 RSREELGKVGSQSSFSGSMEIIEVS ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ...........................................................M..M.M............... 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ......................... // NES-accession: NES-0034 Date: 14 Aug 2002 Protein: PROTEIN KINASE C, IOTA TYPE (EC 2.7.1.-) (NPKC-IOTA) (PROTEIN KINASE C LAMBDA) Organism: Mus musculus (Mouse) DB_reference: SWISS-PROT Q62074, KPCI_MOUSE Necessity: F244A/L246A mutant accumulates in the nucleus. Sufficiency: - Pathway: LMB-sensitive. Location: Predominantly cytoplasmic. Regulation: - Comments: Discrepancy of 9 in amino acid numbering between article and retrieved sequence. References: Perander M, Bjorkoy G and Johansen T, 2001, J Biol Chem 276: 13015-24. Medline: 11115515 Sequence: 586 aa MSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKWIDEEGDPCTVSSQLELEEA 80 FRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGY 160 KCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQTMHPDHTQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLG 240 LQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFF 320 VIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTT 400 STFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAA 480 SVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDI 560 VRKIDQSEFEGFEYINPLLMSAEECV ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ...M.M.......................................................................... 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 .......................... // NES-accession: NES-0035 Date: 14 Aug 2002 Protein: MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 4 (SMAD 4) (MOTHERS AGAINST DPP HOMOLOG 4) (DELETION TARGET IN PANCREATIC CARCINOMA 4) (HSMAD4) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q13485, SMA4_HUMAN Necessity: L146A/L148A abrogates export. Sufficiency: - Pathway: LMB-sensitive. Location: Cytoplasmic. Regulation: Export inhibited by protein-protein interactions. Comments: - References: Watanabe M, Masuyama N, Fukuda M and Nishida E, 2000 EMBO Rep 1: 176-82. Medline: 11265759 Sequence: 552 aa MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDG 80 RLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKD 160 EYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQI 240 ASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAP 320 EYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVR 400 CLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAP 480 AISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD ................................................................................ 80 .................................................................M.M............ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ........................................................................ // NES-accession: NES-0036 Date: 14 Aug 2002 Protein: ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN) (TRIPARTITE MOTIF PROTEIN 27) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P14373, RFP_HUMAN Necessity: Aa 132-255 necessary in a deletional mutant context. L204A/L207A/L209A abrogates export. Sufficiency: - Pathway: LMB-sensitive. Location: - Regulation: NES activated upon PKC alfa activation, but RFP is not a direct substrate of the kinase. Comments: - References: Harbers M, Nomura T, Ohno S, Ishii S, 2001, J Biol Chem 276: 48596-607. Medline: 11591718 Sequence: 513 aa MASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQ 80 LRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRR 160 RAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQ 240 QQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTL 320 DPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTS 400 APQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSG 480 GKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP ................................................................................ 80 ................................................................................ 160 ...........................................M..M.M............................... 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................. // NES-accession: NES-0037 Date: 14 Aug 2002 Protein: RPF1/Nedd4, Ubiquitin-protein ligase (E3) Organism: Homo sapiens (Human) DB_reference: REFSEQ XP_046129.4 Necessity: Aa 293-309 necessary in a deletional mutant context. L305A/I307A leads to whole cell distribution. Sufficiency: - Pathway: LMB-sensitive. Location: Cytoplasmic. Regulation: Constitutive export. Comments: There is no SWISS-PROT entry matching this sequence: The swissprot entry P46934 is stated to be only a fragment, but is longer than the sequence used in the article. The XP_046129 protein though, match the length and residue numbering of the sequence in the article. References: Hamilton MH, Tcherepanova I, Huibregtse JM and McDonnell DP, 2001, J Biol Chem 276: 26324-31. Medline: 11342538 Sequence: 900 aa MATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRV 80 HPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDD 160 NAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQA 240 QRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSS 320 NHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQ 400 SQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWE 480 ERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRI 560 MGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGR 640 VAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGS 720 EIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSA 800 NHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFE 880 ELWDKLQMAIENTQGFDGVD ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................M.M............. 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................................................ 880 .................... // NES-accession: NES-0038 Date: 14 Aug 2002 Protein: TRANSCRIPTIONAL ACTIVATOR PDR4 (YAP-1 PROTEIN) (Yap1p) Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: SWISS-PROT P19880, PDR4_YEAST Necessity: V616A, L619A and C620T abrogates export, whereas C620A does not. Sufficiency: - Pathway: Crm-dependence and interaction with Crm both in vitro and in vivo. Location: Cytoplasm. Regulation: Yap1p responds to oxidative stress. NES masked upon oxidation of cysteines in the NES region. Comments: - References: Yan C, Lee LH and Davis LI, 1998, EMBO J 17: 7416-29. Medline: 9857197 Sequence: 650 aa MSVSTAKRSLDVVSPGSLAEFEGSKSRHDEIENEHRRTGTRDGEDSEQPKKKGSKTSKKQDLDPETKQKRTAQNRAAQRA 80 FRERKERKMKELEKKVQSLESIQQQNEVEATFLRDQLITLVNELKKYRPETRNDSKVLEYLARRDPNLHFSKNNVNHSNS 160 EPIDTPNDDIQENVKQKMNFTFQYPLDNDNDNDNSKNVGKQLPSPNDPSHSAPMPINQTQKKLSDATDSSSATLDSLSNS 240 NDVLNNTPNSSTSMDWLDNVIYTNRFVSGDDGSNSKTKNLDSNMFSNDFNFENQFDEQVSEFCSKMNQVCGTRQCPIPKK 320 PISALDKEVFASSSILSSNSPALTNTWESHSNITDNTPANVIATDATKYENSFSGFGRLGFDMSANHYVVNDNSTGSTDS 400 TGSTGNKNKKNNNNSDDVLPFISESPFDMNQVTNFFSPGSTGIGNNAASNTNPSLLQSSKEDIPFINANLAFPDDNSTNI 480 QLQPFSESQSQNKFDYDMFFRDSSKEGNNLFGEFLEDDDDDKKAANMSDDESSLIKNQLINEEPELPKQYLQSVPGNESE 560 ISQKNGSSLQNADKINNGNDNDNDNDVVPSKEGSLLRCSEIWDRITTHPKYSDIDVDGLCSELMAKAKCSERGVVINAED 640 VQLALNKHMN ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 .......................................................M..MM.................... 640 .......... // NES-accession: NES-0039 Date: 14 Aug 2002 Protein: Putative ATP-dependent RNA helicase An3 Organism: Xenopus laevis (African clawed frog) DB_reference: SWISS-PROT P24346, AN3_XENLA Necessity: Delta 1-21 and L19A/L21A both abrogates export. Sufficiency: - Pathway: Full-length An3 interacts in vitro with Crm1, whereas delta 1-21 aa does not. Location: - Regulation: - Comments: - References: Askjaer P, Bachi A, Wilm M, Bischoff FR, Weeks DL, Ogniewski V, Ohno M, Niehrs C, Kjems J, Mattaj IW, Fornerod M, 1999, Mol Cell Biol 19: 6276-85. Medline: 10454574 Sequence: 697 aa MSHVAVENVLNLDQQFAGLDLNSADAESGVAGTKGRYIPPHLRNKEASRNDSNWDSGRGGNGYINGMQDDRDGRMNGYDR 80 GGYGSRGTGRSDRGFYDRENSGWNSGRDKDAYSSFGSRGDRGKGSLFNERGSGSRRTDDRRQDGFDGMGNRSDKSGFGRF 160 DRGNSRWSDDRNDEDDWSKPLAPNDRVEQELFSGSNTGINFEKYDDIPVEATGSNCPPHIESFHDVTMGEIIMGNIQLTR 240 YTRPTPVQKHAIPIIIEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGDAMKHLQENGRYGRRKQFPLSLVLAPTRELA 320 VQIYEEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEP 400 QIRRIVEQDTMPPKGVRQTMMFSATFPKEIQILARDFLDEYIFLAVGRVGSTSENITQKVVWVEEMDKRSFLLDLLNATG 480 KDSLTLVFVETKKGADALEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLP 560 SDIEEYVHRIGRTGRVGNLGLATSFFNEKNINITKDLLDLLVEAKQEVPSWLENMAYEQHHKSSSRGRSKSRFSGGFGAK 640 DYRQSSGAGSSFGSSRGGRSSGHGGSRGFGGGYGGFYNSDGYGGNYGGSSQVDWWGN ..................M.M........................................................... 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ......................................................... // NES-accession: NES-0040 Date: 14 Aug 2002 Protein: M-phase inducer phosphatase 1/B (Cdc25) Organism: Xenopus laevis (African clawed frog) DB_reference: SWISS-PROT P30309, MPI1_XENLA Necessity: Both L53A/V55A/F57A and L344A/V347A/I349A greatly impair export of full-length fused to GFP. Sufficiency: - Pathway: LMB-sensitivity. Location: Cytoplasmic. Regulation: - Comments: Aa 47-57 is a stronger NES than aa 342-50. References: Kumagai A and Dunphy WG, 1999 Genes Dev 13: 1067-72. Medline: 10323858 Sequence: 550 aa MAESHIMSSEAPPKTNTGLNFRTNCRMVLNLLREKDCSVTFSPEQPLTPVTDLAVGFSNLSTFSGETPKRCLDLSNLGDE 80 TAPLPTESPDRISSGKVESPKAQFVQFDGLFTPDLGWKAKKCPRGNMNSVLPRLLCSTPSFKKTSGGQRSVSNKENEGEL 160 FKSPNCKPVALLLPQEVVDSQFSPTPENKVDISLDEDCEMNILGSPISADPPCLDGAHDDIKMQNLDGFADFFSVDEEEM 240 ENPPGAVGNLSSSMAILLSGPLLNQDIEVSNVNNISLNRSRLYRSPSMPEKLDRPMLKRPVRPLDSETPVRVKRRRSTSS 320 SLQPQEENFQPQRRGTSLKKTLSLCDVDISTVLDEDCGHRQLIGDFTKVYALPTVTGRHQDLRYITGETLAALIHGDFSS 400 LVEKIFIIDCRYPYEYDGGHIKGALNLHRQEEVTDYFLKQPLTPTMAQKRLIIIFHCEFSSERGPKMCRFLREEDRARNE 480 YPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPMHHQDFREELLKFRTKCKTSVGDRKRREQIARIMKL ....................................................M.M.M....................... 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 .......................M..M.M................................................... 400 ................................................................................ 480 ...................................................................... // NES-accession: NES-0041 Date: 14 Aug 2002 Protein: Adenomatous polyposis coli protein (APC protein) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P25054, APC_HUMAN Necessity: Export of either full-length APC or a VP16-NLS-aa1-270 fusion protein, both get abrogated by simultaneous mutation of both NESs (L75A/L77A/L172A/L174A). Sufficiency: Aa 68-77 and 165-74 are each sufficient to mediate export of GST, aa 68-77 being a stronger NES than 165-74. Pathway: LMB-sensitivity: In vivo interaction with Crm is dependent on the intact NES sequences. Location: Cytoplasmic. Regulation: - Comments: 2 NESs. References: Neufeld KL, Nix DA, Bogerd H, Kang Y, Beckerle MC, Cullen BR and White RL, 2000, Proc Natl Acad Sci U S A 97: 12085-90. Medline: 11035805 Sequence: 2843 aa MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSS 80 NFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYA 160 QLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQAT 240 EAERSSQNKHETGSHDAERQNEGQGVGEINMATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLS 320 MLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRG 400 RREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQ 480 VDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSK 560 KTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILR 640 NVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAM 720 GSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQSLYGDYV 800 FDTNRHDDNRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLGNYHPATENPGTSSKRGLQI 880 STTAAQIAKVMEEVSAIHTSQEDRSSGSTTELHCVTDERNALRRSSAAHTHSNTYNFTKSENSNRTCSMPYAKLEYKRSS 960 NDSLNSVSSSDGYGKRGQMKPSIESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGR 1040 QSPSQNERWARPKHIIEDEIKQSEQRQSRNQSTTYPVYTESTDDKHLKFQPHFGQQECVSPYRSRGANGSETNRVGSNHG 1120 INQNVSQSLCQEDDYEDDKPTNYSERYSEEEQHEEEERPTNYSIKYNEEKRHVDQPIDYSLKYATDIPSSQKQSFSFSKS 1200 SSGQSSKTEHMSSSSENTSTPSSNAKRQNQLHPSSAQSRSGQPQKAATCKVSSINQETIQTYCVEDTPICFSRCSSLSSL 1280 SSAEDEIGCNQTTQEADSANTLQIAEIKEKIGTRSAEDPVSEVPAVSQHPRTKSSRLQGSSLSSESARHKAVEFSSGAKS 1360 PSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPP 1440 PPPQTAQTKREVPKNKAPTAEKRESGPKQAAVNAAVQRVQVLPDADTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDV 1520 ELRIMPPVQENDNGNETESEQPKESNENQEKEAEKTIDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKPAQTASK 1600 LPPPVARKPSQLPVYKLLPSQNRLQPQKHVSFTPGDDMPRVYCVEGTPINFSTATSLSDLTIESPPNELAAGEGVRGGAQ 1680 SGEFEKRDTIPTEGRSTDEAQGGKTSSVTIPELDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASASSSAP 1760 NKNQLDGKKKKPTSPVKPIPQNTEYRTRVRKNADSKNNLNAERVFSDNKDSKKQNLKNNSKDFNDKLPNNEDRVRGSFAF 1840 DSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKAKENKESEAKVTSHTELTSNQQSANKTQAIAKQPINR 1920 GQPKPILQKQSTFPQSSKDIPDRGAATDEKLQNFAIENTPVCFSHNSSLSSLSDIDQENNNKENEPIKETEPPDSQGEPS 2000 KPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKPSRLKGDNEKHSPRNMGGILGEDLTLDL 2080 KDIQRPDSEHGLSPDSENFDWKAIQEGANSIVSSLHQAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFT 2160 SNKGPRILKPGEKSTLETKKIESESKGIKGGKKVYKSLITGKVRSNSEISGQMKQPLQANMPSISRGRTMIHIPGVRNSS 2240 SSTSPVSKKGPPLKTPASKSPSEGQTATTSPRGAKPSVKSELSPVARQTSQIGGSSKAPSRSGSRDSTPSRPAQQPLSRP 2320 IQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQMSQQNLTKQTGLSKNASSIPRSESASKG 2400 LNQMNNGNGANKKVELSRMSSTKSSGSESDRSERPVLVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPASPTRSQAQ 2480 TPVLSPSLPDMSLSTHSSVQAGGWRKLPPNLSPTIEYNDGRPAKRHDIARSHSESPSRLPINRSGTWKREHSKHSSSLPR 2560 VSTWRRTGSSSSILSASSESSEKAKSEDEKHVNSISGTKQSKENQVSAKGTWRKIKENEFSPTNSTSQTVSSGATNGAES 2640 KTLIYQMAPAVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKANPNIKDSKDNQAKQNVGNGSVPMRTVGLEN 2720 RLNSFIQVDAPDQKGTEIKPGQNNPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSA 2800 RPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHSGSYLVTSV ...................................................................aaaaaaaMaM... 80 ................................................................................ 160 ....aaaaaaaMaM.................................................................. 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................................................ 880 ................................................................................ 960 ................................................................................ 1040 ................................................................................ 1120 ................................................................................ 1200 ................................................................................ 1280 ................................................................................ 1360 ................................................................................ 1440 ................................................................................ 1520 ................................................................................ 1600 ................................................................................ 1680 ................................................................................ 1760 ................................................................................ 1840 ................................................................................ 1920 ................................................................................ 2000 ................................................................................ 2080 ................................................................................ 2160 ................................................................................ 2240 ................................................................................ 2320 ................................................................................ 2400 ................................................................................ 2480 ................................................................................ 2560 ................................................................................ 2640 ................................................................................ 2720 ................................................................................ 2800 ........................................... // NES-accession: NES-0042 Date: 14 Aug 2002 Protein: Phosphorylated adaptor for RNA export (PHAX) Organism: Mus musculus (Mouse) DB_reference: TrEMBL Q9JJT9 Necessity: V120A/L124A/L127A/M129A prevents Crm-association. Sufficiency: - Pathway: Crm-complex formation. Location: - Regulation: Exported upon phosphorylation. Comments: - References: Ohno M, Segref A, Bachi A, Wilm M and Mattaj IW, 2000, Cell 101: 187-98. Medline: 10786834 Sequence: 385 aa MALEAGDMEEGQLSDSDSDMTVVPSDRPLQMAKVLGGGSAACAPVSHYRTVKHVDSSEESLDSDDDCSLWKRKRQKCHNT 80 PPKPEPFPFGPSGQKTALNGGKKVNNIWGAVLQEQNQDAVATELGILGMEGSIDRSRQSETYNYLLAKKLAKKESQEYTK 160 ELDKDLDEYMHGDKKPGSKEDENGQGHLKRKRPVRDRLGNRVEMNYKGRYEITEEDAPEKVADEIAFRLQEPKKDLIARV 240 VRILGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYVENQKEYENKKAARKRRTQL 320 LGKKMKQAIKSLNFQEDDDTSRETFASDTNEALASLDEAQEGPGETKLDAEEAIEVDHPQDLDIF ................................................................................ 80 .......................................M...M..M.M............................... 160 ................................................................................ 240 ................................................................................ 320 ................................................................. // NES-accession: NES-0043 Date: 14 Aug 2002 Protein: Brain expressed X-linked protein 3 (P75NTR-associated cell death EXECUTOR) (NADE) Organism: Mus musculus (Mouse) DB_reference: TrEMBL Q9WTZ9 Necessity: L94A/L97A/L99A leads to whole cell distribution. Sufficiency: - Pathway: LMB-sensitive. Location: Cytoplasmic. Regulation: - Comments: - References: Mukai J, Shoji S, Kimura MT, Okubo S, Sano H, Suvanto P, Li Y, Irie S and Sato TA, 2002, J Biol Chem 277: 13973-82. Medline: 11830582 Sequence: 124 aa MANVHQENEEMEQPLQNGQEDRPVGGGEGHQPAANNNNNNHNHNHNHHRRGQARRLAPNFRWAIPNRQMNDGLGGDGDDM 80 EMFMEEMREIRRKLRELQLRNCLRILMGELSNHHDHHDEFCLMP ................................................................................ 80 .............M..M.M......................... // NES-accession: NES-0044 Date: 14 Aug 2002 Protein: Open reading frame YDR499w (Cip1p) Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: REFSEQ NP_010787.1 Necessity: L670A/L673A abrogates export Sufficiency: Aa 660-747 fragment interacts with Crm in vitro, in a LMB-sensitive manner. Pathway: LMB-sensitive. Location: Whole cell distribution. Regulation: - Comments: Sequence stated as open reading frame YDR499w by the authors which corresponds to REFSEQ NP_010787.1. No corresponding SWISS-PROT entry. References: Jensen TH, Neville M, Rain JC, McCarthy T, Legrain P and Rosbash M, 2000, Mol Cell Biol 20: 8047-58. Medline: 11027275 Sequence: 747 aa MRRETVGEFSSDDDDDILLELGTRPPRFTQIPPSSAALQTQIPTTLEVTTTTLNNKQSKNDNQLVNQLNKAQGEASMLRD 80 KINFLNIEREKEKNIQAVKVNELQVKHLQELAKLKQELQKLEDEKKFLQMEARGKSKREVITNVKPPSTTLSTNTNTITP 160 DSSSVAIEAKPQSPQSKKRKISDNLLKKNMVPLNPNRIIPDETSLFLESILLHQIIGADLSTIEILNRLKLDYITEFKFK 240 NFVIAKGAPIGKSIVSLLLRCKKTLTLDRFIDTLLEDIAVLIKEISVHPNESKLAVPFLVALMYQIVQFRPSATHNLALK 320 DCFLFICDLIRIYHHVLKVPIHESNMNLHVEPQIFQYELIDYLIISYSFDLLEGILRVLQSHPKQTYMEFFDENILKSFE 400 FVYKLALTISYKPMVNVIFSAVEVVNIITSIILNMDNSSDLKSLISGSWWRDCITRLYALLEKEIKSGDVYNENVDTTTL 480 HMSKYHDFFGLIRNIGDNELGGLISKLIYTDRLQSVPRVISKEDIGMDSDKFTAPIIGYKMEKWLLKLKDEVLNIFENLL 560 MIYGDDATIVNGEMLIHSSKFLSREQALMIERYVGQDSPNLDLRCHLIEHTLTIIYRLWKDHFKQLREEQIKQVESQLIM 640 SLWRFLVCQTETVTANEREMRDHRHLVDSLHDLTIKDQASYYEDAFEDLPEYIEEELKMQLNKRTGRIMQVKYDEKFQEM 720 ARTILESKSFDLTTLEEADSLYISMGL ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ...................aaaaaaaaaaMaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 720 aaaaaaaaaaaaaaaaaaaaaaaaaaa // NES-accession: NES-0045Date: 14 Aug 2002 Protein: Nonstructural protein NS2 Organism: Influenza B virus (strain B/Yamagata/1/73) DB_reference: SWISS-PROT P08014, VNS2_INBYA Necessity: NEP-B can substitute for HIV1 Rev effector domain, whereas M14A/M17A/I19A cannot. Sufficiency: - Pathway: Crm-interaction in 2 hybrid system Location: - Regulation: - Comments: - References: Paragas J, Talon J, O'Neill RE, Anderson DK, Garcia-Sastre A and Palese P, 2001, J Virol 75: 7375-83. Medline: 11462009 Sequence: 122 aa MADNMTTTQIEWRMKKMAIGSSTHSSSVLMKDIQSQFEQLKLRWESYPNLVKSTDYHQRRETIRLVTEELYLLSKRIDDN 80 ILFHKTVIANSSIIADMIVSLSLLETLYEMKDVVEVYSRQCL .............M..M.M............................................................. 80 .......................................... // NES-accession: NES-0046 Date: 14 Aug 2002 Protein: Nonstructural protein NS2 Organism: Influenza A virus (strain A/Puerto Rico/8/34) DB_reference: SWISS-PROT P03508, VNS2_IAPUE Necessity: Fragments containg aa 1-30 can substitute for HIV1 Rev effector domain, whereas fragments lacking aa 1-30 cannot. M16A/M19A/L21A in full-length context abrogates export activity. Sufficiency: Aa 1-30 can substitue for HIV1 Rev effector domain. Aa 11-23 mediates export of GST, but if containg the above- mentioned mutations, export is abrogated. Pathway: - Location: - Regulation: - Comments: - References: O'Neill RE, Talon J and Palese P, 1998, EMBO J 17: 288-96. Medline: 9427762 Sequence: 121 aa MDPNTVSSFQDILLRMSKMQLESSSGDLNGMITQFESLKLYRDSLGEAVMRMGDLHSLQNRNEKWREQLGQKFEEIRWLI 80 EEVRHKLKITENSFEQITFMQALHLLLEVEQEIRTFSFQLI ...............M..M.M........................................................... 80 ......................................... // NES-accession: NES-0047 Date: 14 Aug 2002 Protein: NF-kappaB inhibitor epsilon (NF-kappa-BIE) (I-kappa-B-epsilon) (IkappaBepsilon) (IKB-epsilon) (IKBE) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT O00221, IKBE_HUMAN Necessity: L350A/I352A abrogates export of full-length IKBE. Sufficiency: - Pathway: LMB-sensitive, Crm-interaction in vitro. Location: Cytoplasmic. Regulation: - Comments: - References: Lee SH and Hannink M, 2002, J Biol Chem 277: 23358-66. Medline: 11970947 Sequence: 361 aa MSEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASGPSDGSPQPCTHPPGPVKEPQEKEDADGERADSTYGSSSLTY 80 TLSLLGGPEAEDPPPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCCLALLPQEVLDIQNNLYQTALH 160 LAVHLDQPGAVRALVLKGASRALQDRHGDTALHVACQRQSWPVPAACWKGGPEPGRGTSHSLDLQLQNWQGLACLHIATL 240 QKNQPLMELLLRNGADIDVQEGSSGKTALHLAVETQERGLVQFLLQAGAQVDARMLNGCTPLHLAAGRGLMGISSTLCKA 320 GADSLLRNVEDETPQDLTEESLVLLPFDDLKISGKLLLCTD ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 .............................M.M......... // NES-accession: NES-0048 Date: 14 Aug 2002 Protein: ATP-dependent RNA helicase p54 (Xp54) (DDX6) Organism: Xenopus laevis (African clawed frog) DB_reference: SWISS-PROT P54824, DDX6_XENLA Necessity: L157A/L159A abrogates export of aa 1-164 fragment. Sufficiency: Aa 1-164 mediates export of GFP. Pathway: LMB-sensitive. Location: Cytoplasmic in quiescent and full-grown oocytes, but nuclear in developing oocytes. Regulation: - Comments: Discrepancy of 1 in amino acid numbering between article and retrieved sequence. References: Smillie DA and Sommerville J, 2001, J Cell Sci115: 395-407. Medline: 11839790 Sequence: 481 aa MSTARTENPVLMGMSSQNGQLRGPLKPSAGPGGGGTQTQQINQLKNASTINSGSQQQAQSMSSIIKPGDDWKKTLKLPPK 80 DLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKTGAYLIPLLERLDLK 160 KDCIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHIQ 240 MIVLDEADKLLSQDFMQIMEDIIMTLPKNRQILLYSATFPLSVQKFMTLHLQKPYEINLMEELTLKGVTQYYAYVTERQK 320 VHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQ 400 AVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSSIDKNLYVAEYHSESGEDK 480 P aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 80 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaMa 160 aaaa............................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 . // NES-accession: NES-0049 Date: 14 Aug 2002 Protein: TELOMERASE REVERSE TRANSCRIPTASE (TELOMERASE CATALYTIC SUBUNIT) (HEST2) (TERT) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT O14746, TERT_HUMAN Necessity: - Sufficiency: In vitro Crm-interaction of aa 831-1132 fused to GST was abrogated by L974A/L978A/L980A. Pathway: LMB-sensitive. Location: Cytoplasmic in the absence of 14-3-3, nuclear in the presence of 14-3-3. Regulation: NES masked upon binding of 14-3-3 protein. Comments: - References: Seimiya H, Sawada H, Muramatsu Y, Shimizu M, Ohko K, Yamane K and Tsuruo T, 2000, EMBO J 2000 19: 2652-61. Medline: 10835362 Sequence: 1132 aa MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKEL 80 VARVLQRLCERGAKNVLAFGFALLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFV 160 LVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARRRGGSASRSLPLPKRPRR 240 GAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTP 320 CPPVYAETKHFLYSSGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNH 400 AQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPWQVYGFVRACLRRLVPPGLWGS 480 RHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSF 560 FYVTETTFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNMDYVV 640 GARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTI 720 PQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNE 800 ASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLLVTPHLTHA 880 KTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTF 960 NRGFKAGRNMRRKLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTA 1040 SLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLPGTTLTALEAAAN 1120 PALPSDFKTILD ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ..............................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 880 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 960 aaaaaaaaaaaaaMaaaMaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 1040 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 1120 aaaaaaaaaaaa // NES-accession: NES-0050 Date: 14 Aug 2002 Protein: Ran GTPase activating protein 1 (Rna1p) Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: SWISS-PROT P11745, RNA1_YEAST Necessity: - Sufficiency: Aa 316-57 mediates export of H2B-lacZ fusion. Aa 316-33 and 329-57 each mediates export, aa 329-57 being most effective. L326A/I328A abrogates export of aa 316-33 fragment. Pathway: Crm-dependent Location: Cytoplasmic Regulation: - Comments: 2 overlapping NESs. "S" and "E" in the assignment field, describes start and end of fragments shown to have NES activity. References: Feng W, Benko AL, Lee JH, Stanford DR and Hopper AK, 1999, J Cell Sci 112: 339-47. Medline: 9885287 Sequence: 407 aa MATLHFVPQHEEEQVYSISGKALKLTTSDDIKPYLEELAALKTCTKLDLSGNTIGTEASEALAKCIAENTQVRESLVEVN 80 FADLYTSRLVDEVVDSLKFLLPVLLKCPHLEIVNLSDNAFGLRTIELLEDYIAHAVNIKHLILSNNGMGPFAGERIGKAL 160 FHLAQNKKAASKPFLETFICGRNRLENGSAVYLALGLKSHSEGLKVVKLYQNGIRPKGVATLIHYGLQYLKNLEILDLQD 240 NTFTKHASLILAKALPTWKDSLFELNLNDCLLKTAGSDEVFKVFTEVKFPNLHVLKFEYNEMAQETIEVSFLPAMEKGNL 320 PELEKLEINGNRLDEDSDALDLLQSKFDDLEVDDFEEVDSEDEEGEDEEDEDEDEKLEEIETERLEKELLEVQVDDLAER 400 LAETEIK ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ...........................................................................Saaaa 320 aaaaaMaMSaaaEaaaaaaaaaaaaaaaaaaaaaaaE........................................... 400 ....... // NES-accession: NES-0051 Date: 14 Aug 2002 Protein: Transcription factor IIIA (Factor A) (TFIIIA) Organism: Bufo americanus (American toad) DB_reference: SWISS-PROT P34694, TF3A_BUFAM Necessity: - Sufficiency: Aa 320-339 mediates export of BSA in oocytes and of GST in HeLa cells. Export abrogated by L336A. Pathway: Aa 320-339 can substitute for the HIV-1 Rev effector domain. Location: - Regulation: - Comments: - References: Fridell RA, Fischer U, Luhrmann R, Meyer BE, Meinkoth JL, Malim MH and Cullen BR, 1996, Proc Natl Acad Sci U S A 93: 2936-40. Medline: 8610146 Sequence: 339 aa MGEKLPVVYKRFICSFPDCNATYNKNRKLQAHLCKHTGERPFPCTYEGCEKGFVTLHHLNRHVLSHTGEKPCKCETENCN 80 LAFTTASNMRLHFKRAHSSPAQVYVCYFADCGQQFRKHNQLKIHQYIHTNQQPFKCSHEGCDKCYASPSRLKRHEKTHAG 160 YPCRKDSTCPFVGKTWSDYMKHAAELHSEVTCSICNRTFKRKSFLKEHKKIHREERIVYRCPRENCDRTYTTKFNLKSHI 240 LTFHENLRPFVCEHEGCGKTFAMKQSLDRHFNTHDPEKKKMVKPPRPVRSLASRLSGYKPKKSKKKKKPSQTPAMESQEQ 320 QPDASKADPLPVLENLTLK ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 aaaaaaaaaaaaaaaMaaa // NES-accession: NES-0052 Date: 14 Aug 2002 Protein: Transcription factor IIIA (Factor A) (TFIIIA) (S-TFIIIA/O-TFIIIA) Organism: Xenopus laevis (African clawed frog) DB_reference: SWISS-PROT P03001, TF3A_XENLA Necessity: - Sufficiency: Aa 347-66 mediates export of reporterprotein. Pathway: Aa 347-66 can complement for the HIV-1 Rev effector domain. Location: - Regulation: - Comments: - References: Fridell RA, Fischer U, Luhrmann R, Meyer BE, Meinkoth JL, Malim MH and Cullen BR, 1996, Proc Natl Acad Sci U S A 93: 2936-40. Medline: 8610146 Ward BM and Lazarowitz SG, 1999, Plant Cell 11: 1267-76. Medline: 10402428 Sequence: 366 aa MAAKVASTSSEEAEGSLVTEGEMGEKALPVVYKRYICSFADCGAAYNKNWKLQAHLCKHTGEKPFPCKEEGCEKGFTSLH 80 HLTRHSLTHTGEKNFTCDSDGCDLRFTTKANMKKHFNRFHNIKICVYVCHFENCGKAFKKHNQLKVHQFSHTQQLPYECP 160 HEGCDKRFSLPSRLKRHEKVHAGYPCKKDDSCSFVGKTWTLYLKHVAECHQDLAVCDVCNRKFRHKDYLRDHQKTHEKER 240 TVYLCPRDGCDRSYTTAFNLRSHIQSFHEEQRPFVCEHAGCGKCFAMKKSLERHSVVHDPEKRKLKEKCPRPKRSLASRL 320 TGYIPPKSKEKNASVSGTEKTDSLVKNKPSGTETNGSLVLDKLTIQ ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ..........................aaaaaaaaaaaaaaaaaaaa // NES-accession: NES-0053 Date: 14 Aug 2002 Protein: Ah receptor (Aryl hydrocarbon receptor) (AhR) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P35869, AHR_HUMAN Necessity: - Sufficiency: Aa 55-75 mediates export of GFP-GST fusion. Abrogated by L70A/L72A. Pathway: Export abrogated by competition of the PKI-NES (NES-0001), which indicates that AhR gets exported by the same pathway as PKI. Location: - Regulation: - Comments: - References: Ikuta T, Eguchi H, Tachibana T, Yoneda Y and Kawajiri K, 1998 J Biol Chem 273: 2895-904. Medline: 9446600 Sequence: 848 aa MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAK 80 SFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYE 160 LIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQ 240 GKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGS 320 GYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLTDEEGTEHLRKRNT 400 KLPFMFTTGEAVLYEATNPFPAIMDPLPLRTKNGTSGKDSATTSTLSKDSLNPSSLLAAMMQQDESIYLYPASSTSSTAP 480 FENNFFNESMNECRNWQDNTAPMGNDTILKHEQIDQPQDVNSFAGGHPGLFQDSKNSDLYSIMKNLGIDFEDIRHMQNEK 560 FFRNDFSGEVDFRDIDLTDEILTYVQDSLSKSPFIPSDYQQQQSLALNSSCMVQEHLHLEQQQQHHQKQVVVEPQQQLCQ 640 KMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYP 720 MGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLN 800 KFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL ......................................................aaaaaaaaaaaaaaaMaMaaa..... 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................ // NES-accession: NES-0054 Date: 14 Aug 2002 Protein: Period circadian protein 2 (mPER2) Organism: Mus musculus (Mouse) DB_reference: SWISS-PROT O54943, PER2_MOUSE Necessity: - Sufficiency: Aa 382-469 mediates export of YFP(2)-NLS. Export is abrogated by I464A/L467A/M469K and greatly impaired by M469K alone. Pathway: LMB-sensitive. Location: - Regulation: - Comments: - References: Vielhaber EL, Duricka D, Ullman KS and Virshup DM, 2001 J Biol Chem 276: 45921-7. Medline: 11591712 Sequence: 1257 aa MNGYVDFSPSPTSPTKEPGAPQPTQAVLQEDVDMSSGSSGNENCSTGRDSQGSDCDDNGKELRMLVESSNTHPSPDDAFR 80 LMMTEAEHNPSTSGCSSEQSAKADAHKELIRTLKELKVHLPADKKAKGKASTLATLKYALRSVKQVKANEEYYQLLMSSE 160 SQPCSVDVPSYSMEQVEGITSEYIVKNADMFAVAVSLVSGKILYISNQVASIFHCKKDAFSDAKFVEFLAPHDVSVFHSY 240 TTPYKLPPWSVCSGLDSFTQECMEEKSFFCRVSVGKHHENEIRYQPFRMTPYLVKVQEQQGAESQLCCLLLAERVHSGYE 320 APRIPPEKRIFTTTHTPNCLFQAVDERAVPLLGYLPQDLIETPVLVQLHPSDRPLMLAIHKKILQAGGQPFDYSPIRFRT 400 RNGEYITLDTSWSSFINPWSRKISFIIGRHKVRVGPLNEDVFAAPPCPEEKTPHPSVQELTEQIHRLLMQPVPHSGSSGY 480 GSLGSNGSHEHLMSQTSSSDSNGQEESHRRRSGIFKTSGKIQTKSHVSHESGGQKEASVAEMQSSPPAQVKAVTTIERDS 560 SGASLPKASFPEELAYKNQPPCSYQQISCLDSVIRYLESCSEAATLKRKCEFPANIPSRKATVSPGLHSGEAARPSKVTS 640 HTEVSAHLSSLTLPGKAESVVSLTSQCSYSSTIVHVGDKKPQPELETVEDMASGPESLDGAAGGLSQEKGPLQKLGLTKE 720 VLAAHTQKEEQGFLQRFREVSRLSALQAHCQNYLQERSRAQASDRGLRNTSGLESSWKKTGKNRKLKSKRVKTRDSSEST 800 GSGGPVSHRPPLMGLNATAWSPSDTSQSSCPSAPFPTAVPAYPLPVFQAPGIVSTPGTVVAPPAATHTGFTMPVVPMGTQ 880 PEFAVQPLPFAAPLAPVMAFMLPSYPFPPATPNLPQAFLPSQPHFPAHPTLASEITPASQAEFPSRTSTLRQPCACPVTP 960 PAGTVALGRASPPLFQSRGSSPLQLNLLQLEEAPEGSTGAAGTLGTTGTAASGLDCTSGTSRDRQPKAPPTCNEPSDTQN 1040 SDAISTSSDLLNLLLGEDLCSATGSALSRSGASATSDSLGSSSLGFGTSQSGAGSSDTSHTSKYFGSIDSSENNHKAKMI 1120 PDTEESEQFIKYVLQDPIWLLMANTDDSIMMTYQLPSRDLQAVLKEDQEKLKLLQRSQPRFTEGQRRELREVHPWVHTGG 1200 LPTAIDVTGCVYCESEEKGNICLPYEEDSPSPGLCDTSEAKEEEGEQLTGPRIEAQT ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 .............................................................aaaaaaaaaaaaaaaaaaa 400 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaMaM........... 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................................................ 880 ................................................................................ 960 ................................................................................ 1040 ................................................................................ 1120 ................................................................................ 1200 ......................................................... // NES-accession: NES-0055 Date: 14 Aug 2002 Protein: Nucleoprotein p40 (N protein) Organism: Borna disease virus (BDV) DB_reference: TrEMBL Q91UL8 Necessity: - Sufficiency: Aa 128-41 mediates export of GFP. L128A/L131A/I133A/I136A abrogates export. Pathway: LMB-sensitive. Location: - Regulation: BDV P protein masks the NES of p38 N protein, and prevent its export. Comments: This is the sequence of p40, p38 might be more correct.This sequence fits with the aa-numbering in the paper though. References: Kobayashi T, Kamitani W, Zhang G, Watanabe M, Tomonaga K and Ikuta K, 2001, J Virol 75: 3404-12. Medline: 11238866 Sequence: 370 aa MPPKRRLVDDADAMEDQDLYEPPASLPKLPGKFLQYTVGGSDPHPGIGHEKDIRQNAVALLDQSRRDMFHTVTPSLVFLC 80 LLIPGLHAAFVHGGVPRESYLSTPVTRGEQTVVKTAKFYGEKTTQRDLTELEISSIFSHCCSLLIGVVIGSSSKIKAGAE 160 QIKKRFKTMMAALNRPSHGETATLLQMFNPHEAIDWINGQPWVGSFVLSLLTTDFESPGKEFMDQIKLVASYAQMTTYTT 240 IKEYLAECMDATLTIPVVAYEIRDFLEVSAKLKEEHADLFPFLGAIRHPDAIKLAPRSFPNLASAAFYWSKKENPTMAGY 320 RASTIQPGASVKETQLARYRRREISRGEDGAELSGEISAIMKMIGVTGLN ................................................................................ 80 ...............................................MaaMaMaaMaaaaa................... 160 ................................................................................ 240 ................................................................................ 320 .................................................. // NES-accession: NES-0056 Date: 14 Aug 2002 Protein: Zyxin Organism: Gallus gallus (Chicken) DB_reference: SWISS-PROT Q04584, ZYX_CHICK Necessity: Delta 322-31 abrogates export of full-length Zyxin. Sufficiency: Aa 319-35 mediates export of GST, whereas aa 322-31 does not. Pathway: - Location: Colocalizes with integrin receptors at focal contacts. Regulation: - Comments: - References: Nix DA and Beckerle MC, 1997, J Cell Biol 1138: 1139-47. Medline: 9281590 Sequence: 542 aa MASPGTPGTRMTTTVSINISTPSFYNPQKKFAPVVAPKPKVNPFKTGGTSESSQPQPPGTGAQRAQIGRVGEIPVSVTAE 80 ELPLPPPPPPGEELSFSSNCAFPPPPPPFEEPFPPAPDEAFPSPPPPPPPMFDEGPALQIPPGSTGSVEKPLAPKAHVEI 160 SSAPRDPTPPFPSKFTPKPSGTLSSKPPGLDSTPAPAPWAAPQQRKEPLASVPPPPSLPSQPTAKFTPPPVASSPGSKPG 240 ATVPMAPSNSTRYPTSLQTQFTAPSPSGPLSRPQPPNFTYAQQWERPQVQEKPVPTEKSAAVKDMRRPTADPPKGNSPLT 320 MKEVEELELLTQKLMKDMDHPPPVEAATSELCGFCRKPLSRTQPAVRALDCLFHVECFTCFKCEKQLQGQQFYNVDEKPF 400 CEDCYAGTLEKCSVCKQTITDRMLKATGNSYHPQCFTCVMCHTPLEGASFIVDQANQPHCVDDYHRKYAPRCSVCSEPIM 480 PEPGKDETVRVVALEKNFHMKCYKCEDCGRPLSIEADENGCFPLDGHVLCMKCHTVRAKTAC ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ...............................................................................a 320 aaaaaaaaaaaaaaa................................................................. 400 ................................................................................ 480 .............................................................. // NES-accession: NES-0057 Date: 14 Aug 2002 Protein: Eukaryotic translation initiation factor 3 subunit 6 (eIF-3 p48) (Mammary tumor-associated protein INT-6) (Viral integration site protein INT-6) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q64252, IF36_HUMAN Necessity: Aa 1-10 necessary in a deletional mutant context. Sufficiency: Aa 1-20 mediates export of the nuclear protein DRBP76. Export abrogated by either L6A, L14A or L18A. Pathway: - Location: Cytoplasmic. Regulation: - Comments: - References: Guo J and Sen GC, 2000, J Virol 74: 1892-9. Medline: 10644362 Sequence: 445 aa MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKNLYSDDIPHALREKRTTVVAQ 80 LKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADKHGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPA 160 TDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQP 240 QYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLV 320 NDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSP 400 YQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY aaaaaMaaaaaaaMaaaMaa............................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ............................................. // NES-accession: NES-0058 Date: 14 Aug 2002 Protein: 52 kDa immediate-early phosphoprotein (ORF 57) Organism: Herpesvirus saimiri (strain 11). DB_reference: SWISS-PROT P13199, IE63_HSVSA Necessity: L164A/L166A abrogates export of full-length protein. Sufficiency: Aa 154-170 mediates export of GFP. Pathway: - Location: - Regulation: - Comments: - References: Goodwin DJ, Hall KT, Stevenson AJ, Markham AF and Whitehouse A, 1999, J Virol 73: 10519-24. Medline: 10559371 Sequence: 417 aa MEDIIEGGISSDDDFDSSDSSSDEEESDTSPQIMKSDVTMASPPSTPEPSPDVSASTSNLKRERQRSPITWEHQSPLSRV 80 YRSPSPMRFGKRPRISSNSTSRSCKTSWADRVREAAAQRRPSRPFRKPYSHPRNGPLRNGPPRAPPLLKLFDISILPKSG 160 EPKLFLPVPSLPCQEAEKTNDKYVLAMAQRAMHDVPISSKQLTANLLPVKFKPLLSIVRYTPNYYYWVSMRKETIASANL 240 CTVAAFLDESLCWGQQYLKNDFIFSENGKDIILDTSSALLSQLVHKIKMLPFCHCLMQTTPQDHIVKQVCYLIASNNRIL 320 DAVRYLQTSVIKSPIVLLLAYAVCLPAAIICTKNETQLYSHCMRILKEYRPGDVMNILHESLTQHLNKCPSSTCAYTTRA 400 IVGTKANTTGLFFLPTQ ................................................................................ 80 .........................................................................aaaaaaa 160 aaaMaMaaaa...................................................................... 240 ................................................................................ 320 ................................................................................ 400 ................. // NES-accession: NES-0059 Date: 14 Aug 2002 Protein: Importin beta-1 subunit (Karyopherin beta-1 subunit) (Importin95) (Kap95p) Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: SWISS-PROT Q06142, IMB1_YEAST Necessity: Aa 55-65 necessary in aa 1-77 fragment. L63A greatly impairs export of full-length Kap95p. Sufficiency: Aa 1-77 mediates export of GST. Pathway: - Location: - Regulation: - Comments: - References: Iovine MK and Wente SR, 1997 J Cell Biol 137: 797-811. Medline: 9151683 Sequence: 861 aa MSTAEFAQLLENSILSPDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKLEGRILAALTLKNELVSKDSVKTQQF 80 AQRWITQVSPEAKNQIKTNALTALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVDNTGAEQPENVKRASLLALG 160 YMCESADPQSQALVSSSNNILIAIVQGAQSTETSKAVRLAALNALADSLIFIKNNMEREGERNYLMQVVCEATQAEDIEV 240 QAAAFGCLCKIMSLYYTFMKPYMEQALYALTIATMKSPNDKVASMTVEFWSTICEEEIDIAYELAQFPQSPLQSYNFALS 320 SIKDVVPNLLNLLTRQNEDPEDDDWNVSMSAGACLQLFAQNCGNHILEPVLEFVEQNITADNWRNREAAVMAFGSIMDGP 400 DKVQRTYYVHQALPSILNLMNDQSLQVKETTAWCIGRIADSVAESIDPQQHLPGVVQACLIGLQDHPKVATNCSWTIINL 480 VEQLAEATPSPIYNFYPALVDGLIGAANRIDNEFNARASAFSALTTMVEYATDTVAETSASISTFVMDKLGQTMSVDENQ 560 LTLEDAQSLQELQSNILTVLAAVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVFYAISALAASLGKGFEKYLETF 640 SPYLLKALNQVDSPVSITAVGFIADISNSLEEDFRRYSDAMMNVLAQMISNPNARRELKPAVLSVFGDIASNIGADFIPY 720 LNDIMALCVAAQNTKPENGTLEALDYQIKVLEAVLDAYVGIVAGLHDKPEALFPYVGTIFQFIAQVAEDPQLYSEDATSR 800 AAVGLIGDIAAMFPDGSIKQFYGQDWVIDYIKRTRSGQLFSQATKDTARWAREQQKRQLSL aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaaaaaaaaaaaaaa.. 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ............................................................. // NES-accession: NES-0060 Date: 14 Aug 2002 Protein: BR1 protein Organism: Squash leaf curl virus DB_reference: SWISS-PROT P21935, VBR1_SLCV Necessity: Aa 204-226 necessary in a deletional mutant context. Export of full-length BR1 is abrogated by L216A/L217A/L220A. Sufficiency: Aa 204-283 mediates export of GUS-NLS. Pathway: - Location: - Regulation: - Comments: Discrepancy of 27 in amino acid numbering between article and retrieved sequence. References: Ward BM and Lazarowitz SG, 1999, Plant Cell 11: 1267-76. Medline: 10402428 Sequence: 283 aa MRRIHILDNTCIIYTYIHCAVAIASSLMYSTSNRRGRSQTQRGSHVRRTGVKRSYGAARGDDRRRPNVVSKTQVEPRMTI 80 QRVQENQFGPEFVLSQNSALSTFVTYPSYVKTVPNRTRTYIKLKRVRFKGTLKIERGQGDTIMDGPSSNIEGVFSMVIVV 160 DRKPHVSQSGRLHTFDELFGARIHCHGNLSVVPALKDRYYIRHVTKRVVSLEKDTLLIDLHGTTQLSNKRYNCWASFSDL 240 ERDSCNGVYGNITKNALLVYYCWLSDAQSKASTYVSFELDYLG ................................................................................ 80 ................................................................................ 160 ...........................................aaaaaaaaaaaaMMaaMaaaaaaaaaaaaaaaaaaaa 240 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa // NES-accession: NES-0061 Date: 14 Aug 2002 Protein: Fragile X mental retardation 1 protein (Protein FMR-1) (FMRP) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q06787, FMR1_HUMAN. Necessity: Delta 425-41 results in nuclear localization. Sufficiency: Aa 425-41 mediates export of BSA. Export is abrogated by L430A/L432A/L435A. Pathway: - Location: Cytoplasmic, ribosome-associated. Regulation: - Comments: - References: Eberhart DE, Malter HE, Feng Y, Warren ST, 1996, Hum Mol Genet 5: 1083-91. Medline: 8842725 Sequence: 632 aa MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWW 80 LAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVT 160 YDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGAN 240 IQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGKVIGKNGKLIQEIVDKSGVVRVRIEA 320 ENEKNVPQEEEIMPPNSLPSNNSRVGPNAPEEKKHLDIKENSTHFSQPNSTKVQRVLVASSVVAGESQKPELKAWQGMVP 400 FVFVGTKDSIANATVLLDYHLNYLKEVDQLRLERLQIDEQLRQIGASSRPPPNRTDKEKSYVTDDGQGMGRGSRPYRNRG 480 HGRRGPGYTSGTNSEASNASETESDHRDELSDWSLAPTEEERESFLRRGDGRRRGGGGRGQGGRGRGGGFKGNDDHSRTD 560 NRPRNPREAKGRTTDGSLQIRVDCNNERSVHTKTLQNTSSEGSRLRTGKDRNQKKEKPDSVDGQQPLVNGVP ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ........................aaaaaMaMaaMaaaaaa....................................... 480 ................................................................................ 560 ........................................................................ // NES-accession: NES-0062 Date: 14 Aug 2002 Protein: Transcriptional regulator IE63 (VMW63) (ICP27) Organism: Herpes simplex virus (type 1 / strain 17). DB_reference: SWISS-PROT P10238, IE63_HSV11 Necessity: Delta aa 4-27 and L8R/L11R/L13R/L15R both abrogate export. Sufficiency: Aa 5-17 mediates export of GFP. Pathway: PKI-NES (NES-0001) can substitute, indicating common pathway. Location: - Regulation: - Comments: - References: Sandri-Goldin RM, 1998, Genes Dev 12:868-79. Medline: 9512520 Sequence: 512 aa MATDIDMLIDLGLDLSDSDLDEDPPEPAESRRDDLESDSSGECSSSDEDMEDPHGEDGPEPILDAARPAVRPSRPEDPGV 80 PSTQTPRPTERQGPNDPQPAPHSVWSRLGARRPSCSPEQHGGKVARLQPPPTKAQPARGGRRGRRRGRGRGGPGAADGLS 160 DPRRRAPRTNRNPGGPRPGAGWTDGPGAPHGEAWRGSEQPDPPGGQRTRGVRQAPPPLMTLAIAPPPADPRAPAPERKAP 240 AADTIDATTRLVLRSISERAAVDRISESFGRSAQVMHDPFGGQPFPAANSPWAPVLAGQGGPFDAETRRVSWETLVAHGP 320 SLYRTFAGNPRAASTAKAMRDCVLRQENFIEALASADETLAWCKMCIHHNLPLRPQDPIIGTTAAVLDNLATRLRPFLQC 400 YLKARGLCGLDELCSRRRLADIKDIASFVFVILARLANRVERGVAEIDYATLGVGVGEKMHFYLPGACMAGLIEILDTHR 480 QECSSRVCELTASHIVAPPYVHGKYFYCNSLF ....aaaMaaMaMaMaa............................................................... 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................ // NES-accession: NES-0063 Date: 14 Aug 2002 Protein: Nonstructural protein NS1 Organism: Influenza A virus (strain A/Alaska/6/77). DB_reference: SWISS-PROT P03494, VNS1_IAALA Necessity: Aa 134-47 necessary in a deletional mutant context. Sufficiency: Aa 138-47 mediates export of GST. Export abrogated by L144A and L146A, whereas I145A had no effect. Pathway: - Location: Nuclear. Regulation: NES masked by adjacent sequence, and NS1 (148-161). Exported upon unmasking (during infection). Comments: - References: Li Y, Yamakita Y and Krug RM, 1998, Proc Natl Acad Sci U S A 95: 4864-9. Medline: 9560194 Sequence: 237 aa MDSNTVSSFQVDCFLWHVRKQVVDQELGDAPFLDRLRRDQRSLRGRGSTLGLDIEAATHVGKQIVEKILKEESDEALKMT 80 MASTPASRYITDMTIEELSRDWFMLMPKQKVEGPLCIRMDQAIMEKNIMLKANFSVIFDRLETLILLRAFTEEGAIVGEI 160 SPLLSFPGHTIEDVKNAIGVLIGGLEWNDNTVRVSKTLQRFAWGSSNENGGPPLTPKQKRKMARTARSKVRRDKMAD ................................................................................ 80 .........................................................aaaaaaMaMa............. 160 ............................................................................. // NES-accession: NES-0064 Date: 14 Aug 2002 Protein: MAP kinase kinase 6 (MKK6) Organism: Cyprinus carpio (Common carp) DB_reference: TrEMBL Q9I959 Necessity: Both delta1-57, L46A/L50A and L53A/L55A abrogate export of MKK6. Sufficiency: Aa 45-57 mediates export of ovalbumin. Export abrogated by L46A/L50A/L53A/L55A. Pathway: - Location: Cytoplasmic. Regulation: - Comments: - References: Hashimoto H, Fukuda M, Matsuo Y, Yokoyama Y, Nishida E, Toyohara H and Sakaguchi M, 2000, Eur J Biochem 2000 267: 4362-71. Medline: 10880959 Sequence: 404 aa MMGHACGSSASDLLQMERTYLALLSRLPLSHLHSLSSHSLALSHLLSQDLEPLALSDRKCSSDRESRNSMGSRNKAGKKK 80 PPLKLPKEVFEKPQPAPTPPRDLDSKACVTIGDKNFVVKADDLEQIGELGRGAYGVVDKMRHVPSGVIMAVKRIRATVNT 160 QEQKRLLMDLDISMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYKQVHEKGMTIPEDILGKITVSIVKALEHLH 240 SNLSVIHRDVKPSNVLINMQGQVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPETNQKGYNVKSDIWSLGITMIEL 320 AILRFPYDSWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTSQCLRKNSKERPTYTELMQHPFFTIHDSKDTDVASFVKS 400 ILGD ............................................aMaaaMaaMaMaa....................... 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 .... // NES-accession: NES-0065 Date: 14 Aug 2002 Protein: LIM protein SLIMMER Organism: Homo sapiens (Human) DB_reference: TrEMBL O95212 Necessity: - Sufficiency: Aa 254-65 sufficient in a deletional mutant context. Pathway: - Location: Nuclear in myoblasts and cytoplasmic in myotubes Regulation: - Comments: - References: Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM and Mitchell CA, 1999, J Biol Chem 274: 27083-91. Medline: 10480922 Sequence: 323 aa MAEKFDCHYCRDPLQGKKYVQKDGHHCCLKCFDKFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETF 80 VAKDNKILCNKCTTREDSPKCKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQVIGTGSFFPKGEDFYCVTCHETKFAK 160 HCVKCNKAITSGGITYQDQPWHADCFVCVTCSKKLAGQRFTAVEDQYYCVDCYKNFVAKKCAGCKNPITGKRTVSRVSHP 240 VSKARKPPVCHGKRLPLTLFPSANLRGRHPGGERTCPSWVVVLYRKNRSLAAPRGPGLVKAPVWWPMKDNPGTTTASTAK 320 NAP ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ..............aaaaaaaaaaa....................................................... 320 ... // NES-accession: NES-0066 Date: 14 Aug 2002 Protein: Homeobox protein engrailed-2 (Gg-En-2) Organism: Gallus gallus (Chicken) DB_reference: SWISS-PROT Q05917, HME2_CHICK Necessity: Delta 232-43 impaires export, but shuttling is still detected in 20% of heterokaryons. Sufficiency: Aa 229-48 mediates export of reporterprotein. Pathway: - Location: Nuclear Regulation: - Comments: - References: Maizel A, Bensaude O, Prochiantz A, Joliot A, 1999, Development 126: 3183-90. Medline: 10375508 Sequence: 288 aa MEEGGRSPREEAAEPQESGGDAEPGGGRRALLLPPGDPPHPHPHPHRITNFFIDNILRPEFGRRKEAGGTAGEPRRPGAE 80 SRRSPAAAAPAPGAPVPGGGGGGGGGSPGRGEGGPAALALHGAAKKGGDPAALEAALKARGLSGAELSVSSDSDSSQAGS 160 NAGNQPMLWPAWVYCTRYSDRPSSPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELGLNE 240 SQIKIWFQNKRAKIKKATGSKNSLAVHLMAQGLYNHSTTAKDGKSDSE ................................................................................ 80 ................................................................................ 160 ....................................................................aaaaaaaaaaaa 240 aaaaaaaa........................................ // NES-accession: NES-0067 Date: 14 Aug 2002 Protein: Nonsense-mediated mRNA decay protein 3 (Nmd3p) Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: SWISS-PROT P38861, NMD3_YEAST Necessity: Delta 474-518 greatly impairs export, while delta 439-518 completely abrogates export. Sufficiency: Aa 435-518 mediates export of NLS-GFP fusion. Pathway: LMB-sensitive, Crm-dependent. Location: Cytoplasmic. Regulation: - Comments: The assignment field for this sequenceis not accurate: The experimental data propose 2 NESs, located from aa 445-56 and 492-503, respectively. References: Ho JH, Kallstrom G, Johnson AW, 2000, J Cell Biol 151: 1057-66. Medline: 11086007 Gadal O, Strauss D, Kessl J, Trumpower B, Tollervey D and Hurt E, 2001, Mol Cell Biol 21: 3405-15. Medline: 11313466 Sequence: 518 aa MEFTPIDPHQHQNAATLLCCNCGTPIDGSTGLVMCYDCIKLTVDITQGIPREANISFCRNCERFLQPPGQWIRAELESRE 80 LLAICLRRLKGLTFVRLVDASFIWTEPHSRRIRIKLTVQGEAMTNTIIQQTFEVEYIVIAMQCPDCARSYTTNTWRATVQ 160 IRQKVPHKRTFLFLEQLILKHNAHVDTISISEAKDGLDFFYAQKNHAVKMIDFLNAVVPIKHKKSEELISQDTHTGASTY 240 KFSYSVEIVPICKDDLVVLPKKLAKSMGNISQFVLCSKISNTVQFMDPTTLQTADLSPSVYWRAPFNALADVTQLVEFIV 320 LDVDSTGISRGNRVLADITVARTSDLGVNDQVYYVRSHLGGICHAGDSVMGYFIANSNYNSDLFDGLNIDYVPDVVLVKK 400 LYQRKSKKSRHWKLKRMAKEHKDIDASLDYNSRAQKQEMERAEKDYELFLQELEEDAELRQSVNLYKNREANVPPEEHEM 480 DEDEDEDAPQINIDELLDELDEMTLEDGVENTPVESQQ ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ..................................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 480 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa // NES-accession: NES-0068 Date: 14 Aug 2002 Protein: Forkhead box protein O3A (Forkhead in rhabdomyosarcoma-like 1) (AF6q21 protein) (FKHRL1) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT O43524, FXO3_HUMAN Necessity: Export abrogated by simultaneous mutation of both putative NESs (369-78 and 386-96) by M376A/L378A/L382A/L390A/L391A/I394A/L396A. Sufficiency: - Pathway: LMB-sensitive. Location: Nuclear. Regulation: Exported upon phosphorylation by Akt and only when bound to 14-3-3 protein (the latter shown not to be providing the NES). Comments: - References: Brunet A, Kanai F, Stehn J, Xu J, Sarbassova D, Frangioni JV, Dalal SN, DeCaprio JA, Greenberg ME, Yaffe MB, 2002 J Cell Biol 156: 817-28. Medline: 11864996 Sequence: 673 aa MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIG 80 GGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNL 160 SYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDG 240 GKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTV 320 SGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQ 400 PSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLS 480 HSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLS 560 ESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDF 640 NFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 .......................................................M.M...M.......MM..M.M.... 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................. // NES-accession: NES-0069 Date: 14 Aug 2002 Protein: Histone deacetylase 5 (HD5) (Antigen NY-CO-9) (HDAC5) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q9UQL6, HDA5_HUMAN Necessity: Delta 1081-1122, V1086 and L1092A all greatly impaires or abrogates CaMK-mediated nuclear export. Sufficiency: Aa 1081-22 NOT suffcient to mediate export of GFP, but suffcient to mediate export of MITR - another 14-3-3 binding protein - upon CaMK stimulation. Pathway: LMB-sensitive. Location: Nuclear. Regulation: Export upon phosphorylation by CaMK. Comments: Results indicative of additional sequence information being necessary for export mediated by this NES. References: McKinsey TA, Zhang CL and Olson EN, 2001, Mol Cell Biol 21: 6312-21. Medline: 11509672 Sequence: 1122 aa MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQEL 80 LALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRL 160 EQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKL 240 PLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPS 320 SPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAE 400 RQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATS 480 MRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQ 560 EVLLGEGALTMPREGSTESESTQEDLEEEDEEEDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQ 640 VYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQE 720 TGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSA 800 VRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQA 880 FYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVS 960 AGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPN 1040 INAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEP 1120 AL ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................................................ 880 ................................................................................ 960 ................................................................................ 1040 ........................................aaaaaMaaaaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaa 1120 aa // NES-accession: NES-0070 Date: 14 Aug 2002 Protein: Histone deacetylase 4 (HD4) (HA6116) (HDAC4) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT P56524, HDA4_HUMAN Necessity: V1056A greatly impaires, whereas L1062 abrogates the CaMK mediated export (McKinsey et al. 2001). Aa 1040-84 necessary in a deletional mutant context (Wang et al. 2001). Sufficiency: Aa 1044-69 mediates export of GFP, but this export is abrogated by either M1059A, L1062A, V1064A or V1066A (Wang et al. 2001). Pathway: LMB-sensitivity. Location: Nuclear. Regulation: Exported upon phosphorylation by CaMK (McKinsey et al. 2001). Comments: - References: McKinsey TA, Zhang CL and Olson EN, 2001, Mol Cell Biol 21: 6312-21. Medline: 11509672 Wang AH and Yang XJ, 2001, Mol Cell Biol 21: 5992-6005. Medline: 11486037 Sequence: 1084 aa MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQLQQELLALKQ 80 KQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEK 160 GKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFP 240 LRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDVTDSACSSAPGSGPSSPNNSSGSVSAENGIAPAV 320 PSIPAETSLAHRLVAREGSAAPLPLYTSPSLPNITLGLPATGPSAGTAGQQDTERLTLPALQQRLSLFPGTHLTPYLSTS 400 PLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHL 480 VIQQQHQQFLEKHKQQFQQQQLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEAHAQAGVQVKQ 560 EPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGGHRPLSRAQSSPASATFP 640 VSVQEPPTKPRFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHT 720 LLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPP 800 GHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEV 880 GTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQL 960 MGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGR 1040 SLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 ................................................................................ 720 ................................................................................ 800 ................................................................................ 880 ................................................................................ 960 ................................................................................ 1040 ...aaaaaaaaaaaaaaaMaaMaMaMaaa............... // NES-accession: NES-0071 Date: 14 Aug 2002 Protein: Transcription factor E2F4 (E2F-4) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q16254, E2F4_HUMAN Necessity: I68A/L70A, delta 84-105 and L97S/I98R all lead to nuclear accumulation, which is abolished by overexpression of Crm1. Sufficiency: Since neither of the above-mentioned mutants are cytoplasmic, it seems that they are not 2 independently sufficient NESs. Pathway: LMB-sensitive, Crm-dependent. Location: Cytoplasmic. Regulation: - Comments: Atypical long NES. References: Gaubatz S, Lees JA, Lindeman GJ, Livingston DM, 2001, Mol Cell Biol 21: 1384-92. Medline: 11158323 Sequence: 413 aa MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQ 80 WKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAP 160 SGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEAS 240 RPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSN 320 SNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESE 400 GVCDLFDVPVLNL ...................................................................M.M.......... 80 ................MM.............................................................. 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ............. // NES-accession: NES-0072 Date: 14 Aug 2002 Protein: DNA damage checkpoint protein rad24 Organism: Schizosaccharomyces pombe (Fission yeast) DB_reference: SWISS-PROT P42656, RA24_SCHPO Necessity: I222A/L226A greatly impairs export. Sufficiency: - Pathway: Crm-dependency. Location: Cytoplasmic. Regulation: - Comments: - References: Lopez-Girona A, Furnari B, Mondesert O and Russell P, 1999, Nature 397: 172-5. Medline: 9923681 Sequence: 270 aa MSTTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQ 80 VELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEI 160 ATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAEYS 240 AAAAGGNTEGAQENAPSNAPEGEAEPKADA ................................................................................ 80 ................................................................................ 160 .............................................................M...M.............. 240 .............................. // NES-accession: NES-0073 Date: 14 Aug 2002 Protein: Serine/threonine protein kinase 4 (STE20-like kinase MST1) (MST-1) (Mammalian STE20-like protein kinase 1) (Serine/threonine protein kinase Krs-2) Organism: Homo sapiens (Human) DB_reference: SWISS-PROT Q13043, STK4_HUMAN Necessity: Only simultaneous mutation of both NESs abrogates export (M368A/I370A/W439A/V441A or M368A/I370A/V441A/L444A). Sufficiency: The fact that MST1 is still excluded from the nucleus when only 1 NES is mutated at the time, is indicative of each NES being sufficient. Pathway: LMB-sensitive. Location: Cytoplasmic. Regulation: Dimerization facilitates cytoplasmic retention, whereas phosphorylation stimulates import. Comments: - References: Lee KK and Yonehara S, 2002, J Biol Chem 277: 12351-8. Medline: 11805089 Sequence: 487 aa METVQLRNPPRRQLKKLDEDSLTKQPEEVFDVLEKLGEGSYGSVYKAIHKETGQIVAIKQVPVESDLQEIIKEISIMQQC 80 DSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYLHFMRKIHRDIKAGNILLNTE 160 GHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNP 240 PPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPFVRSAKGVSILRDLINEAMDVKLKRQESQQREVDQDDEENS 320 EEDEMDSGTMVRAVGDEMGTVRVASTMTDGANTMIEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFE 400 QKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAK 480 KRRQQNF ................................................................................ 80 ................................................................................ 160 ................................................................................ 240 ................................................................................ 320 ...............................................M.M.............................. 400 ......................................M.M..M.................................... 480 ....... // NES-accession: NES-0074 Date: 14 Aug 2002 Protein: Iron-regulated transcriptional repressor AFT1 Organism: Saccharomyces cerevisiae (Baker's yeast) DB_reference: SWISS-PROT P22149, AFT1_YEAST. NLSdb NLS:270 Necessity: L99A and L102A constitutively nuclear even in iron-replete cells. Sufficiency: - Pathway: - Location: Nuclear in iron-deplete cells, and cytoplasmic in iron-replete cells. Regulation: - Comments: - References: Yamaguchi-Iwai Y, Ueta R, Fukunaka A, Sasaki R, 2002, J Biol Chem 277: 18914-8. Medline: 11877447 Sequence: 690 aa MEGFNPADIEHASPINSSDSHSSSFVYALPKSASEYVVNHNEGRASASGNPAAVPSPIMTLNLKSTHSLNIDQHVHTSTS 80 PTETIGHIHHVEKLNQNNLIHLDPVPNFEDKSDIKPWLQKIFYPQGIELVIERSDAFKVVFKCKAAKRGRNARRKRKDKP 160 KGQDHEDEKSKINDDELEYASPSNATVTNGPQTSPDQTSSIKPKKKRCVSRFNNCPFRVRATYSLKRKRWSIVVMDNNHS 240 HQLKFNPDSEEYKKFKEKLRKDNDVDAIKKFDELEYRTLANLPIPTATIPCDCGLTNEIQSFNVVLPTNSNVTSSASSST 320 VSSISLDSSNASKRPCLPSVNNTGSINTNNVRKPKSQCKNKDTLLKRTTMQNFLTTKSRLRKTGTPTSSQHSSTAFSGYI 400 DDPFNLNEILPLPASDFKLNTVTNLNEIDFTNIFTKSPHPHSGSTHPRQVFDQLDDCSSILFSPLTTNTNNEFEGESDDF 480 VHSPYLNSEADFSQILSSAPPVHHDPNETHQENQDIIDRFANSSQEHNEYILQYLTHSDAANHNNIGVPNNNSHSLNTQH 560 NVSDLGNSLLRQEALVGSSSTKIFDELKFVQNGPHGSQHPIDFQHVDHRHLSSNEPQVRSHQYGPQQQPPQQLQYHQNQP 640 HDGHNHEQHQTVQKDMQTHESLEIMGNTLLEEFKDIKMVNGELKYVKPED ................................................................................ 80 ..................M..M.......................................................... 160 ................................................................................ 240 ................................................................................ 320 ................................................................................ 400 ................................................................................ 480 ................................................................................ 560 ................................................................................ 640 .................................................. // NES-accession: NES-0075 Date: 14 Aug 2002 Protein: Transcriptional regulator IE63 homolog (Diffuse early antigen) (SM) (BMLF1) Organism: Epstein-barr virus (strain B95-8) (Human herpesvirus 4) DB_reference: SWISS-PROT Q04360, IE63_EBV Necessity: Delta 227-36 and V184A/T185A/L186A/L193A/T194A/L195A both abrogate shuttling in heterokaryon assay, whereas L193A/T194A/L195A does not. Sufficiency: Aa 186-95 and aa 177-86 are each independently sufficient to mediate export of a GFP-NLS-PK fusion protein. Pathway: LMB sensitive. Location: Nuclear. Regulation: - Comments: 2 overlapping NESs (designated "A/M" and "a/M", respectively). Discrepancy of 41 in amino acid numbering between article and retrieved sequence. References: Chen L, Liao G, Fujimuro M, Semmes OJ and Hayward SD, 2001, Virology 288: 119-28. Medline: 11543664 Sequence: 438 aa MEGSEEHSTDGEISSSEEEDEDPTPAHAIPARPSSVVITPTSASFVIPRKKWDLQDKTVTLHRSPLCRDEDEKEETGNSS 80 YTRGHKRRRGEVHGCTDESYGKRRHLPPGARAPRAPRAPRVPRAPRSPRAPRSNRATRGPRSESRGAGRSTRKQARQERS 160 QRPLPNKPWFDMSLVKPVSKITFVTLPSPLASLTLEPIQDPFLQSMLAVAAHPEIGAWQKVQPRHELRRSYKTLREFFTK 240 STNKDTWLDARMQAIQNAGLCTLVAMLEETIFWLQEITYHGDLPLAPAEDILLACAMSLSKVILTKLKELAPCFLPNTRD 320 YNFVKQLFYITCATARQNKVVETLSSSYVKQPLCLLAAYAAVAPAYINANCRRRHDEVEFLGHYIKNYNPGTLSSLLTEA 400 VETHTRDCRSASCSRLVRAILSPGTGSLGLFFVPGLNQ ................................................................................ 80 ................................................................................ 160 ................AAAAAAAMMMaaaaaaMMM............................................. 240 ................................................................................ 320 ................................................................................ 400 ...................................... //