NES-accession: NES-0001
Date: 14 Aug 2002
Protein: cAMP-dependent Protein Kinase Inhibitor, alpha form (PKI-alpha).
Organism: Homo sapiens (Human) / Oryctolagus cuniculus (Rabbit).
DB_reference: SWISS-PROT P04541, IPKA_HUMAN.
Necessity: Export abrogated by the following mutations: L37A/L39A/L41A,
L37A/L41A, L41A/L44A, L44A/I46A, L44A and I46A.
Sufficiency: Aa 37-46 mediates export of GST.
Pathway: LMB-sensitive.
Location: -
Regulation: -
Comments: -
References: Wen W, Meinkoth JL, Tsien RY and Taylor SS, 1995,
Cell 82: 463-73. Medline: 7634336
Henderson and Eleftheriou, 2000,
Exp. Cell Res. 256: 213-224. Medline: 10739668
Sequence: 75 aa
TDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEEDAQRSSTEQSGEAQGEAAKSES
....................................MaMaMaaMaM.............................
//
NES-accession: NES-0002
Date: 14 Aug 2002
Protein: Cellular tumor antigen p53 (Tumor suppressor p53)
(Phosphoprotein p53) (Antigen NY-CO-13)
Organism: Homo sapiens (Human).
DB_reference: SWISS-PROT P04637, P53_HUMAN. NLSdb NLS:76, NLS:127
Necessity: L348A/L350A does not shuttle in heterokaryon assay (Stommel et
al. 1999)
Sufficiency: Aa 339-352 mediates export of BSA (stommel et al. 1999).
Zhang and Xiong 2001 further shows aa 11-27 to mediate export
of GFP, which is abrogated by the L14Q/F19S or L22Q/W23S
mutations. This is argued though, by Inoue et al. 2002, who
shows that p53 aa 7-27 cannot function as an autonomous NES.
Pathway: LMB-sensitive
Location: Nucleus
Regulation: Regulation by masking the NES upon tetramerization (Stommel
et al. 1999). Phosphorylation of Ser15, abrogates export (Zhang
and Xiong 2001)
Comments: There is controversy regarding the N-terminal NES (Zhang and
Xiong 2001, Inoue et al. 2002), why it has not been included
in the assignment field.
References: Stommel JM, Marchenko ND, Jimenez GS, Moll UM, Hope TJ and
Wahl GM, 1999
EMBO J 18: 1660-72. Medline: 10075936
Zhang Y and Xiong Y, 2001,
Science 292: 1910-5. Medline: 11397945
Inoue T, Stuart J, Leno R, Maki CG, 2002,
J. Biol. Chem. 277: 15053-60. Medline: 11847229
Sequence: 393 aa
MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAP 80
TPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAM 160
AIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS 240
SCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKK 320
KPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
..................aaaaaaaaaMaMaa.........................................
//
NES-accession: NES-0003
Date: 14 Aug 2002
Protein: Ubiquitin-protein ligase E3 Mdm2 (p53-binding protein Mdm2)
(Oncoprotein Mdm2) (Double minute 2 protein) (Hdm2)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q00987, MDM2_HUMAN
Necessity: Mutation of L199A/I202A greatly impaires nuclear shuttling in a
heterokaryon-assay
Sufficiency: Aa 188-202 mediates export of GFP
Aa 191-205 mediates export of GST
Pathway: Rev-like pathway
Location: Nucleus
Regulation: -
Comments: Discrepancy on Aa numbering: There is a difference of 6 in the
numbering of amino acids between this sequence and these articles
References: Roth J, Dobbelstein M, Freedman DA, Shenk T, Levine AJ, 1998
EMBO J 17: 554-64. Medline: 9430646
Zhang Y and Xiong Y, 2001
Science 292: 1910-5. Medline: 11397945
Sequence: 491 aa
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSND 80
LLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSS 160
RRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDS 240
VSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCN 320
RCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTS 400
SSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQ 480
PIQMIVLTYFP
................................................................................ 80
................................................................................ 160
...........................aaaaaaaaaaaMaaM...................................... 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
...........
//
NES-accession: NES-0004
Date: 14 Aug 2002
Protein: Xsmad4a
Organism: Xenopus laevis (African clawed frog).
DB_reference: TrEMBL Q9W639
Necessity: Necessary signal within the region of aa 134-320
(Deletional mutant context)
Sufficiency: Aa 137-46 mediates export of ovalbumin
Pathway: LMB-sensitive
Location: Cytoplasmic
Regulation: -
Comments: -
References: Watanabe M, Masuyama N, Fukuda M and Nishida E, 2000
EMBO Rep 1: 176-82. Medline: 11265759
Sequence: 549 aa
MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQ 80
VAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSSAPSGLLVKDEYG 160
HDYAEGQQSISAGDGLSIQTIQHPPSSRSSTETYSNPALLVPSEPSTPNPTSFSSIPVPSTSQPASLLAATHNDGLLSIA 240
PVPPQGTQNGFSAQPATYHHNSTTTWTGSRTAAYTPNMSHHPNGHLQHHPPMPHPGHYWPVHNELAFQPPISNHPAPDYW 320
CSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLS 400
DHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAIS 480
LSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD
................................................................................ 80
........................................................aaaaaaaaaa.............. 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
.....................................................................
//
NES-accession: NES-0005
Date: 14 Aug 2002
Protein: p120 catenin isoform 3AB
Organism: Homo sapiens (Human)
DB_reference: GenBank AF062338.1
Necessity: Exon B necessary for export
Sufficiency: Aa 755-71 mediates export of ovalbumin.
Pathway: LMB-sensitive
Location: Isoforms containing exon B are exclusively cytoplasmic.
Isoforms lacking exon B are equally distributed between nucleus
and cytoplam
Regulation: Regulation by alternative splicing. NES located in exon B.
Comments: 32 different human isoforms
References: van Hengel J, Vanhoenacker P, Staes K and van Roy F, 1999
Proc Natl Acad Sci U S A 96: 7980-5. Medline: 10393933
Sequence: 861 aa
MQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRD 80
FRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQP 160
QVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQ 240
HERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPK 320
KEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEV 400
IIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVC 480
LLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRI 560
YISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELI 640
GKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQ 720
TIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTP 800
NERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
..................................aaaaaaaaaMaMaaaaa..........
//
NES-accession: NES-0006
Date: 14 Aug 2002
Protein: MAP KINASE-ACTIVATED PROTEIN KINASE 2
(MAPK-ACTIVATED PROTEIN KINASE 2) (MAPKAP KINASE 2) (MAPKAPK-2)
Organism: Mus musculus (Mouse)
DB_reference: SWISS-PROT P49138, MKK2_MOUSE
Necessity: A L345A mutation of the otherwise exclusively cytoplasmic T319E
mutant, results in nuclear localization
Sufficiency: Aa 330-353 is sufficient to mediate export of GFP, whereas
neither aa 341-351 nor aa 337-353 are
Pathway: LMB-sensitive
Location: Predominantly nuclear
Regulation: Phosphorylation of T319, but not T207, unmasks NES and allows
export
Comments: Atypical NES with weak similarity to EAIV and FIV Rev NESs.
Discrepancy of 2 in amino acid numbering between article and
retrieved sequence
References: Engel K, Kotlyarov A and Gaestel M, 1998
EMBO J 17: 3363-71. Medline: 9628873
Sequence: 385 aa
IRGSPGQTPPAPFPSPPPPAPAQPPPPFPQFHVKSGLQIRKNAITDDYKVTSQVLGLGINGKVLRIFDKRTQQKFALKML 80
QDCPKARREVELTWRASQCPHIVHIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQ 160
YLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYI 240
LLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTP 320
LHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARAVEDAALAH
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
.........aaaaaaaaaaaaaaaMaaaaaaaa................................
//
NES-accession: NES-0007
Date: 14 Aug 2002
Protein: PROTO-ONCOGENE TYROSINE-PROTEIN KINASE ABL1 (P150) (C-ABL)
Organism: Mus musculus (Mouse)
DB_reference: SWISS-PROT P00520, ABL1_MOUSE
Necessity: L1090A mutation results in exclusively nuclear localization of
both full-length protein and aa 998-1079.
Sufficiency: Aa 1078-96 mediates export of GST
Pathway: LMB-sensitive
Location: Predominantly nuclear
Regulation: -
Comments: Discrepancy of 26 in amino acid numbering between article and
retrieved sequence.There is no sequence in the databases with
the same aa numbering as the in the article
References: Taagepera S, McDonald D, Loeb JE, Whitaker LL, McElroy AK,
Wang JY and Hope TJ, 1998
Proc Natl Acad Sci U S A 95: 7457-62. Medline 9636171
Sequence: 1123 aa
MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTL 80
SITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQ 160
RSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERT 240
DITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTY 320
GNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAK 400
FPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNP 480
SDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESD 560
ALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRE 640
LCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASC 720
MPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPLPGWLKKNEEAAEEGFKDT 800
ESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPG 880
RDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQS 960
TAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNS 1040
EQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDI 1120
VRR
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................................................ 880
................................................................................ 960
................................................................................ 1040
.....................................aaaaaaaaaaaaMaaaaaa........................ 1120
...
//
NES-accession: NES-0008
Date: 14 Aug 2002
Protein: G2/MITOTIC-SPECIFIC CYCLIN B1
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P14635, CGB1_HUMAN
Necessity: V149A/L151A/V153A mutation abrogates export of full-length
protein
Sufficiency: Aa 141-52 mediates export of ovalbumin
Pathway: LMB-sensitive
Location: Cytoplasmic during S and G(2)-phases, but nuclear during
prophase
Regulation: -
Comments: -
References: Toyoshima F, Moriguchi T, Wada A, Fukuda M and Nishida E, 1998
EMBO J 17: 2728-35. Medline: 9582266
Sequence: 433 aa
MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLP 80
KPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAED 160
GADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVP 240
KKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQH 320
TLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTV 400
KNKYATSKHAKISTLPQLNSALVQDLAKAVAKV
................................................................................ 80
............................................................aaaaaaaaMaMaM....... 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
.................................
//
NES-accession: NES-0009
Date: 14 Aug 2002
Protein: Actin
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: SWISS-PROT P02579, ACT_YEAST
Necessity: Mutation of both NESs (I178A/L180A/V219A/L221A), reults in
exclusively nuclear localization. Mutation of one NES at the
time results in inhibition of export
Sufficiency: Both NESs individually sufficient to mediate export of ovalbumin.
Shown with peptides of mammalian actin alfa corresponding to
Sc actin aa 170-81 and aa 211-22 respectively
Pathway: LMB-sensitive
Location: Cytoplasmic
Regulation: -
Comments: 2 NESs in this sequence.
References: Wada A, Fukuda M, Mishima M and Nishida E, 1998
EMBO J 17: 1635-41. Medline: 9501085
Sequence: 375 aa
MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWD 80
DMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVT 160
HVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY 240
ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITAL 320
APSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF
................................................................................ 80
................................................................................ 160
.........aaaaaaaaMaMa.............................aaaaaaaaMaMa.................. 240
................................................................................ 320
.......................................................
//
NES-accession: NES-0010
Date: 14 Aug 2002
Protein: HIV-1 Rev protein
Organism: Human immunodeficiency virus type 1 (CDC-451 isolate) (HIV-1)
DB_reference: SWISS-PROT P05865, REV_HV1C4. NLSdb NLS:166
Necessity: -
Sufficiency: Aa 75-83 mediates export of GST and BSA. Export abrogated by the
L78D/E79L and L83A mutations.
Pathway: LMB-sensitive (Henderson and Eleftheriou 2000).
Location: -
Regulation: -
Comments: -
References: Fischer U, Huber J, Boelens WC, Mattaj IW and Luhrmann R, 1995
Cell 82: 475-83. Medline: 7543368
Henderson BR and Eleftheriou A, 2000
Exp Cell Res 2000 256: 213-24, Medline: 10739668
Sequence: 116 aa
MAGRSGDSDEELIKTVRIIKHLYQSNPPPKPEGTRQARRNRRRRWRERQRRIHSISAWLLSTCLGRSAEPVPLQLPPLER 80
LTLDCSEDCGTSGTQGVGSPQILVESPAVLESGTKE
..........................................................................aaaMMa 80
aaM.................................
//
NES-accession: NES-0011
Date: 14 Aug 2002
Protein: Rex protein
Organism: Human T-lymphotropic virus 1
DB_reference: TrEMBL Q82233
Necessity: -
Sufficiency: Aa 81-94 mediates export of GST. Export abrogated by
L90A/L92A.
Pathway: -
Location: -
Regulation: -
Comments: -
References: Bogerd HP, Fridell RA, Benson RE, Hua J and Cullen BR, 1996
Mol Cell Biol 16: 4207-14. Medline: 8754820
Sequence: 189 aa
MPKTRRRPRRSQRKRPPTPWPTSQGLDRVFFSDTQSTCLETVYKATGAPSLGDYVRPAYIVTPYWPPVQSIRSPGTPSMD 80
ALSAQLYSSLSLDSPPSPPREPLRPSRSLPRQSLIQPPTFHPPSSRPCANTPPSEMDTWNPPLGSTSQPCLFQTPDSGPK 160
TCTPSGEAPLSACTSTSFPPPSPGPSCPT
...............................................................................a 80
aLaaaLaaaMaMaa.................................................................. 160
.............................
//
NES-accession: NES-0012
Date: 14 Aug 2002
Protein: Dual specificity mitogen-activated protein kinase kinase 1
(MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1)
(MAPK/ERK kinase 1) (MEK1)
Organism: Xenopus laevis (African clawed frog)
DB_reference: SWISS-PROT Q05116, MPK1_XENLA
Necessity: Delta 31-50, L32A/L36A and L39A/L41A all lead to whole cell
distribution
Sufficiency: Aa 32-44 mediates export of ovalbumin, which is abrogated
by L32A/L36A/L39A/L41A
Pathway: LMB-sensitive
Location: Cytoplasmic
Regulation: -
Comments: Discrepancy of 1 in amino acid numbering between article and
retrieved sequence
References: Fukuda M, Gotoh I, Gotoh Y and Nishida E, 1996
J Biol Chem 271: 20024-8. Medline: 8702720
Fukuda M, Asano S, Nakamura T, Adachi M, Yoshida M,
Yanagida M and Nishida E, 1997
Nature 390: 308-11. Medline: 9384386
Sequence: 394 aa
PKKKPTPIQLNPNPEGTAVNGTPTAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKVSELGAGNGGV 80
VFKVSHKPTSLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGKI 160
PEKILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYS 240
VQSDIWSMGLSLVEMAIGRYPIPPPDAKELELIFGCSVERDPASSELAPRPRPPGRPISSYGPDSRPPMAIFELLDYIVN 320
EPPPKLPSGVFGAEFQDFVNKCLVKNPAERADLKQLMVHSFIKQSELEEVDFAGWLCSTMGLKQPSTPTHAAGV
...............................MaaaMaaMaMaaa.................................... 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
..........................................................................
//
NES-accession: NES-0013
Date: 14 Aug 2002
Protein: EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15
(PROTEIN EPS15) (AF-1P PROTEIN)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P42566, EP15_HUMAN
Necessity: Aa 766-896 necessary in full-length context. Aa 849-896
necessary in a deletional mutant context. I893A, L882A/L884A and
I886A/L888A leads to nucelar accumulation. Aa 890-96 indirectly
necessary, since aa 766-896 is sufficient to mediate export of
GFP, whereas aa 766-890 is not
Sufficiency: Aa 766-896 is mediates export of GFP, whereas aa 766-890 does not.
Pathway: LMB-sensitive
Location: Cytoplasmic
Regulation: -
Comments: -
References: Poupon V, Polo S, Vecchi M, Martin G, Dautry-Varsat A,
Cerf-Bensussan N, Di Fiore PP and Benmerah A, 2002
J Biol Chem 277: 8941-8. Medline: 11777906
Sequence: 896 aa
MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNKQEFFVALRLV 80
ACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKL 160
PVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMD 240
GFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNI 320
IGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDE 400
LDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQ 480
HLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPA 560
RSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKID 640
PFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESF 720
GGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPF 800
KLNDPFQPFPGNDSPKEKDPEMFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQE 880
DLELAIALSKSEISEA
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
.............................................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 800
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 880
aMaMaMaMaaaaMaaa
//
NES-accession: NES-0014
Date: 14 Aug 2002
Protein: Breast cancer type 1 susceptibility protein (BRCA1)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P38398, BRC1_HUMAN. NLSdb NLS:49
Necessity: L86A/I90A abrogates export of full-length protein
Sufficiency: Aa 81-99 mediates export of GFP
Pathway: LMB-sensitive and Crm-dependent
Location: Whole cell distribution in most cells, but also exclusively
either nuclear or cytoplasmic in some cells
Regulation: -
Comments: -
References: Rodriguez JA, Henderson BR, 2000
J Biol Chem 275: 38589-96. Medline: 10991937
Sequence: 1863 aa
MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFS 80
QLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLG 160
TVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQ 240
PSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNR 320
WAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHD 400
GESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTEN 480
LIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGD 560
SIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQ 640
IDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKE 720
FVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNK 800
CVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEE 880
ECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRG 960
NETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEAS 1040
SSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTV 1120
NTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQ 1200
GYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKAS 1280
QEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQS 1360
MDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALE 1440
DLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNR 1520
NYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSAL 1600
KVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLI 1680
TEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKI 1760
FRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLD 1840
SVALYQCQELDTYLIPQIPHSHY
................................................................................ 80
aaaaaMaaaMaaaaaaaaa............................................................. 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................................................ 880
................................................................................ 960
................................................................................ 1040
................................................................................ 1120
................................................................................ 1200
................................................................................ 1280
................................................................................ 1360
................................................................................ 1440
................................................................................ 1520
................................................................................ 1600
................................................................................ 1680
................................................................................ 1760
................................................................................ 1840
.......................
//
NES-accession: NES-0015
Date: 14 Aug 2002
Protein: Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (RelA)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q04206, TF65_HUMAN. NLSdb NLS:17
Necessity: Delta 440-43 abrogates export of full-length protein
Sufficiency: Aa 436-45 is mediates export of a p50-GFP fusion construct
Pathway: LMB-sensitive
Location: Cytoplasmic when not complexed with p50 subunit of NF-kappa-B
Regulation: -
Comments: -
References: Harhaj EW and Sun SC, 1999
Mol Cell Biol 19: 7088-95. Medline: 10490645
Sequence: 551 aa
MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKD 80
PPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLC 160
FQVTVRDPSGRPLRLPPVLPHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFS 240
QADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSG 320
PTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMV 400
SALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQ 480
GIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
...................................aaaaMMMMaa................................... 480
.......................................................................
//
NES-accession: NES-0016
Date: 14 Aug 2002
Protein: Heat shock factor protein HSF30 (Heat shock transcription factor
30) (HSTF 30) (Heat stress transcription factor) (HsfA2)
Organism: Lycopersicon peruvianum (Peruvian tomato)
DB_reference: SWISS-PROT P41152, HSF3_LYCPE
Necessity: Deletion of the most C-terminal 8 residues or mutation of
L344A/L348A/L351A abrogates export
Sufficiency: C-terminal 23 aa mediates export of the otherwise completely
nuclear HsfB1.
Pathway: LMB-sensitive
Location: Cytosolic
Regulation: Becomes nuclear upon coexpression of HsfA1
Comments: -
References: Heerklotz D, Doring P, Bonzelius F, Winkelhaus S
and Nover L, 2001
Mol Cell Biol 21: 1759-68. Medline: 11238913
Sequence: 351 aa
MEDVMKVKVEEDGIPTAVLPMEGLHDVGPPPFLSKTYEMVEDSSTDQVISWSTTRNSFIVWDSHKFSTTLLPRFFKHSNF 80
SSFIRQLNTYGFRKVDPDRWEFANEGFLGGQKHLLKTIKRRRNVGQSMNQQGSGACIEIGYYGMEEELERLKRDKNVLMT 160
EIVKLRQQQQSTRNQIIAMGEKIETQERKQVQMMSFLAKIFSNPTFLQQYLDKQVHRKDKQRIEVGQKRRLTMTPSVTGS 240
DQPMNYSSSLQESEAELASIEMLFSAAMDNESSSNVRPDSVVTANGTDMEPVADDIWEELLSEDLISGDRAAEEVVVVEQ 320
PEFDVEVEDLVVKTPEWGEELQDLVDQLGFL
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
.......aaaaaaaaaaaaaaaaMaaaMaaM
//
NES-accession: NES-0017
Date: 14 Aug 2002
Protein: AP-1-like transcription factor (Pap1)
Organism: Schizosaccharomyces pombe (Fission yeast)
DB_reference: SWISS-PROT Q01663, AP1_SCHPO
Necessity: Delta 488-544 abrogates export
Sufficiency: Aa 515-533 mediates export of GST-GFP fusion. Export
abrogated by L522A, L526A, F517A/I519A and C523S/C532S and
greatly impaired by I519A and C532S
Pathway: Crm-dependent, LMB-sensitive
Location: Cytoplasmic
Regulation: Regulated by oxidation of cysteine in the NES region
Comments: -
References: Kudo N, Taoka H, Toda T, Yoshida M and Horinouchi S, 1999
J Biol Chem 274: 15151-8. Medline: 10329722
Sequence: 552 aa
MSGQTETLSSTSNIPIAKAEPEQSADFSASHKKRGPVSDRSSRRTSSEEVDLMPNVDDEVDGDVKPKKIGRKNSDQEPSS 80
KRKAQNRAAQRAFRKRKEDHLKALETQVVTLKELHSSTTLENDQLRQKVRQLEEELRILKDGSFTFEMSLPHRNPSLSSL 160
PTTGFSSNFAHMKDGISPQSNLHLSPNSIEKPNMHQNVLHNDRSADNLNHRYQVPPTLVDSNSAQGTLSPETPSSSDSPS 240
NLYLNYPKRKSITHLHHDCSALSNGENGEDVADGKQFCQKLSTACGSIACSMLTKTTPHRASVDILSNLHESTVSPPMAD 320
ESVQRSSEVSKSIPNVELSLNVNQQFVSPFGGTDSFPLPTDTGLDSLFEPDSAIENSHLKNVVMEPELFQAWREPAESLD 400
KEFFNDEGEIDDVFHNYFHNSNENGDLITNSLHGLDFLENANESFPEQMYPFIKHNKDYISNHPDEVPPDGLPQKGKHDT 480
SSQMPSENEIVPAKERAYLSCPKVWSKIINHPRFESFDIDDLCSKLKNKAKCSSSGVLLDERDVEAALNQFN
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
..................................aaMaMaaMMaaMaaaaaMa...................
//
NES-accession: NES-0018
Date: 14 Aug 2002
Protein: Signal transducer and activator of transcription 1-alpha/beta
(Transcription factor ISGF-3 components p91/p84)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P42224, STA1_HUMAN
Necessity: L199A greatly impairs export
Sufficiency: Aa 197-205 sufficient to mediate export of GFP
Pathway: LMB-sensitivity, Crm-interaction
Location: -
Regulation: -
Comments: -
References: Mowen K and David M, 2000
Mol Cell Biol 20: 7273-81. Medline: 10982844
Sequence: 750 aa
MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQ 80
HNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEI 160
KSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWK 240
RRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVE 320
RQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSL 400
AAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAE 480
PRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIES 560
ILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELS 640
AVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTT 720
DNLLPMSPEEFDEVSRIVGSVEFDSMMNTV
................................................................................ 80
................................................................................ 160
....................................aaMaaaaaa................................... 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
..............................
//
NES-accession: NES-0019
Date: 14 Aug 2002
Protein: Metal-regulatory transcription factor (MTF1)
Organism: Homo sapiens (Human)
DB_reference: TrEMBL Q14872
Necessity: L338A/L341A/L343A/L344A abrogates export of full-length MTF1
Sufficiency: Aa 336-44 mediates nuclear exclusion of tandem eGFP
Pathway: LMB-sensitive
Location: -
Regulation: -
Comments: -
References: Saydam N, Georgiev O, Nakano MY, Greber UF and Schaffner W, 2001
J Biol Chem 276: 25487-95. Medline: 11306562
Sequence: 753 aa
MGEHSPDNNIIYFEAEEDELTPDDKMLRFVDKNGLVPSSSGTVYDRTTVLIEQDPGTLEDEDDDGQCGEHLPFLVGGEEG 80
FHLIDHEAMSQGYVQHIISPDQIHLTINPGSTPMPRNIEGATLTLQSECPETKRKEVKRYQCTFEGCPRTYSTAGNLRTH 160
QKTHRGEYTFVCNQEGCGKAFLTSHSLRIHVRVHTKEKPFECDVQGCEKAFNTLYRLKAHQRLHTGKTFNCESEGCSKYF 240
TTLSDLRKHIRTHTGEKPFRCDHDGCGKAFAASHHLKTHVRTHTGERPFFCPSNGCEKTFSTQYSLKSHMKGHDNKGHSY 320
NALPQHNGSEDTNHSLCLSDLSLLSTDSELRENSSTTQGQDLSTISPAIIFESMFQNSDDTAIQEDPQQTASLTESFNGD 400
AESVSDVPPSTGNSASLSLPLVLQPGLSEPPQPLLPASAPSAPPPAPSLGPGSQQAAFGNPPALLQPPEVPVPHSTQFAA 480
NHQEFLPHPQAPQPIVPGLSVVAGASASAAAVASAVAAPAPPQSTTEPLPAMVQTLPLGANSVLTNNPTITITPTPNTAI 560
LQSSLVMGEQNLQWILNGATSSPQNQEQIQQASKVEKVFFTTAVPVASSPGSSVQQIGLSVPVIIIKQEEACQCQCACRD 640
SAKERASSRRKGCSSPPPPEPSPQAPDGPSLQLPAQTFSSAPVPGSSSSTLPSSCEQSRQAETPSDPQTETLSAMDVSEF 720
LSLQSLDTPSNLIPIEALLQGEEEMGLTSSFSK
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
...............aaMaaMaMM........................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
.................................
//
NES-accession: NES-0020
Date: 14 Aug 2002
Protein: Heat shock protein SSB1 (Cold-inducible protein YG101) (Ssb1)
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: SWISS-PROT P11484, HS75_YEAST
Necessity: Delta 546-613 in full-length context leads to whole cell
distribution
Sufficiency: Aa 546-99 mediates complete export of GFP-protein A fusion.
Export mediated by aa 546-86 is less efficient. Delta 574-87
in a 545-612 context abrogates export
Pathway: Crm-dependent
Location: Cytoplasmic
Regulation: -
Comments: -
References: Shulga N, James P, Craig EA and Goldfarb DS, 1999
J Biol Chem 274: 16501-7. Medline: 10347213
Sequence: 612 aa
AEGVFQGAIGIDLGTTYSCVATYESSVEIIANEQGNRVTPSFVAFTPEERLIGDAAKNQAALNPRNTVFDAKRLIGRRFD 80
DESVQKDMKTWPFKVIDVDGNPVIEVQYLEETKTFSPQEISAMVLTKMKEIAEAKIGKKVEKAVITVPAYFNDAQRQATK 160
DAGAISGLNVLRIINEPTAAAIAYGLGAGKSEKERHVLIFDLGGGTFDVSLLHIAGGVYTVKSTSGNTHLGGQDFDTNLL 240
EHFKAEFKKKTGLDISDDARALRRLRTAAERAKRTLSSVTQTTVEVDSLFDGEDFESSLTRARFEDLNAALFKSTLEPVE 320
QVLKDAKISKSQIDEVVLVGGSTRIPKVQKLLSDFFDGKQLEKSINPDEAVAYGAAVQGAILTGQSTSDETKDLLLLDVA 400
PLSLGVGMQGDMFGIVVPRNTTVPTIKRRTFTTCADNQTTVQFPVYQGERVNCKENTLLGEFDLKNIPMMPAGEPVLEAI 480
FEVDANGILKVTAVEKSTGKSSNITISNAVGRLSSEEIEKMVNQAEEFKAADEAFAKKHEARQRLESYVASIEQTVTDPV 560
LSSKLKRGSKSKIEAALSDALAALQIEDPSADELRKAEVGLKRVVTKAMSSR
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
.............aaaaaaaaaaaaaa.........................
//
NES-accession: NES-0021
Date: 14 Aug 2002
Protein: RNA export factor GLE1
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: SWISS-PROT Q12315, GLE1_YEAST. NLSdb NLS:270
Necessity: Delta 351-58 is inviable. Each of the mutantions L351A, L353A,
L356A, and L358A lead to inhibition of poly(a)+ RNA export
Sufficiency: Aa 350-58 mediates export of HSA (human serum albumin). Each of
the above-mentioned mutants abrogates export
Pathway: -
Location: Predominantly at nuclear pore complexes
Regulation: -
Comments: -
References: Murphy R and Wente SR, 1996
Nature 383: 357-60. Medline: 8848052
Sequence: 538 aa
MRFVFDEVFNSDTDSPEFEETCSTTSSTSSQCPTPEPSPAIKLPSFTKVGTKKLVNESVVILDPALENALRDLNLQSKLI 80
PINEPIVAASSIIVPHSTNMPLPRASHSSLLDNAKNSNATAPLLEAIEESFQRKMQNLVLANQKEIQSIRENKRRVEEQR 160
KRKEEEERKRKEAEEKAKREQELLRQKKDEEERKRKEAEAKLAQQKQEEERKKIEEQNEKERQLKKEHEAKLLQQKDKLG 240
KAVTNFDKISKMFWHYKDKIAQIKQDIVLPIKKADVNVRNLLSRHKRKINPKFGQLTNSNQQLFKIQNELTQLINDTKGD 320
SLAYHWILNFIAKAVVHQAETEVRVKPESALPLGKLTLYLLVQFPELQELFMARLVKKCPFVIGFTCEIDTEKGRQNMGW 400
KRNNENKWEDNTSYDERMGGILSLFAIITRLQLPQEFITTTSHPFPIALSWHILARICNTPLNLITNTHFVILGSWWDAA 480
AVQFLQAYGNQASKLLILIGEELTSRMAEKKYVGAARLRILLEAWQNNNMESFPEMSP
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
.............................aMaMaaMaM.......................................... 400
................................................................................ 480
..........................................................
//
NES-accession: NES-0022
Date: 14 Aug 2002
Protein: G2/mitotic-specific cyclin B1
Organism: Xenopus laevis (African clawed frog)
DB_reference: SWISS-PROT P13350, CGB1_XENLA
Necessity: F112A in full-length context severely impaires export.
Sufficiency: Aa 76-125 mediates export of GST.
Pathway: Competition by PKI-NES, Crm-interaction, LMB-sensitivity.
Location: Cytoplasmic during interphase.
Regulation: Possibly by phosphorylation.
Comments: -
References: Yang J, Bardes ES, Moore JD, Brennan J, Powers MA and
Kornbluth S, 1998
Genes Dev 12: 2131-43. Medline: 9679058
Sequence: 397 aa
MSLRVTRNMLANAENNVKTTLAGKRVVATKPGLRPRTALGDIGNKAEVKVPTKKELKPAVKAAKKAKPVDKLLEPLKVIE 80
ENVCPKPAQVEPSSPSPMETSGCLPDELCQAFSDVLIHVKDVDADDDGNPMLCSEYVKDIYAYLRSLEDAQAVRQNYLHG 160
QEVTGNMRAILIDWLVQVQMKFRLLQETMFMTVGIIDRFLQEHPVPKNQLQLVGVTAMFLAAKYEEMYPPEIGDFTFVTD 240
HTYTKAQIRDMEMKILRVLKFAIGRPLPLHFLRRASKIGEVTAEQHSLAKYLMELVMVDYDMVHFTPSQIAAASSCLSLK 320
ILNAGDWTPTLHHYMAYSEEDLVPVMQHMAKNIIKVNKGLTKHLTVKNKYASSKQMKISTIPQLRSDVVVEMARPLM
...........................................................................aaaaa 80
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaaaaaaaaaaaa................................... 160
................................................................................ 240
................................................................................ 320
.............................................................................
//
NES-accession: NES-0023
Date: 14 Aug 2002
Protein: Period circadian protein 1 (Circadian pacemaker protein Rigui)
(mPER) (M-Rigui)
Organism: Mus musculus (Mouse)
DB_reference: SWISS-PROT O35973, PER1_MOUSE
Necessity: I493A/L496A abrogates export of full-length PER1.
Sufficiency: Delta 1-484 is excluded from the nucleus, whereas delta 1-495
has a hole cell distribution. Aa 485-98 mediates export of
YFP(2)-NLS, but export is abrogated by I493A/L496A/L498K.
Pathway: LMB-sensitive
Location: -
Regulation: Phosphorylation by casein kinase I epsilon masks NLS and results
in cytoplasmic accumulation.<
Comments: -
References: Vielhaber EL, Duricka D, Ullman KS and Virshup DM, 2001
J Biol Chem 276: 45921-7. Medline: 11591712
Sequence: 1291 aa
MSGPLEGADGGGDPRPGEPFCPGGVPSPGAPQHRPCPGPSLADDTDANSNGSSGNESNGPESRGASQRSSHSSSSGNGKD 80
SALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMTALRELKLRLPPERRGKGRSG 160
TLATLQYALACVKQVQANQEYYQQWSLEEGEPCAMDMSTYTLEELEHITSEYTLRNQDTFSVAVSFLTGRIVYISEQAGV 240
LLRCKRDVFRGARFSELLAPQDVGVFYGSTTPSRLPTWGTGTSAGSGLKDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTP 320
YVTKIRVSDGAPAQPCCLLIAERIHSGYEAPRIPPDKRIFTTRHTPSCLFQDVDERAAPLLGYLPQDLLGAPVLLFLHPE 400
DRPLMLAIHKKILQLAGQPFDHSPIRFCARNGEYVTMDTSWAGFVHPWSRKVAFVLGRHKVRTAPLNEDVFTPPAPSPAP 480
SLDSDIQELSEQIHRLLLQPVHSSSPTGLCGVGPLMSPGPLHSPGSSSDSNGGDAEGPGPPAPVTFQQICKDVHLVKHQG 560
QQLFIESRAKPPPRPRLLATGTFKAKVLPCQSPNPELEVAPVPDQASLALAPEEPERKETSGCSYQQINCLDSILRYLES 640
CNIPSTTKRKCASSSSYTASSASDDDKQRAGPVPVGAKKDPSSAMLSGEGATPRKEPVVGGTLSPLALANKAESVVSVTS 720
QCSFSSTIVHVGDKKPPESDIIMMEDLPGLAPGPAPSPAPSPTVAPDPTPDAYRPVGLTKAVLSLHTQKEEQAFLNRFRD 800
LGRLRGLDTSSVAPSAPGCHHGPIPPGRRHHCRSKAKRSRHHHHQTPRPETPCYVSHPSPVPSSGPWPPPPATTPFPAMV 880
QPYPLPVFSPRGGPQPLPPAPTSVSPATFPSPLVTPMVALVLPNYLFPTPPSYPYGVSQAPVEGPPTPASHSPSPSLPPP 960
PLSPPHRPDSPLFNSRCSSPLQLNLLQLEESPRTEGGAAAGGPGSSAGPLPPSEETAEPEARLVEVTESSNQDALSGSSD 1040
LLELLLQEDSRSGTGSAASGSLGSGLGSGSGSGSHEGGSTSASITRSSQSSHTSKYFGSIDSSEAEAGAARARTEPGDQV 1120
IKCVLQDPIWLLMANADQRVMMTYQVPSRDAASVLKQDRERLRAMQKQQPRFSEDQRRELGAVHSWVRKGQLPRALDVMA 1200
CVDCGSSVQDPGHSDDPLFSELDGLGLEPMEEGGGEGGGCGVGGGGGDGGEEAQTQIGAKGSSSQDSAMEEEEQGGGSSS 1280
PALPAEENSTS
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
....aaaaaaaaMaaMaM.............................................................. 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................................................ 880
................................................................................ 960
................................................................................ 1040
................................................................................ 1120
................................................................................ 1200
................................................................................ 1280
...........
//
NES-accession: NES-0024
Date: 14 Aug 2002
Protein: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase
delta 1 (PLC-delta-1) (Phospholipase C-delta-1) (PLC-III)
Organism: Rattus norvegicus (Rat)
DB_reference: SWISS-PROT P10688, PID1_RAT
Necessity: L172A/I174A greatly impairs export of full-length protein.
Sufficiency: Aa 164-77 mediates export of ovalbumin, whereas the mutant
peptide L165A/L169A/L172A/LI174A does not.
Pathway: LMB-sensitive.
Location: Predominantly at the plasma membrane.
Regulation: Proposal of calciumregulated mechanism based on structural
comparison with EF-hand structure.
Comments: NES located in EF-hand.
References: Yamaga M, Fujii M, Kamata H, Hirata H and Yagisawa H, 1999
J Biol Chem 274: 28537-41. Medline: 10497218
Sequence: 756 aa
MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHR 80
TEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVQGLRKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKM 160
NFKELKDFLKELNIQVDDGYARKIFRECDHSQTDSLEDEEIETFYKMLTQRAEIDRAFEEAAGSAETLSVERLVTFLQHQ 240
QREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMDQPLSHYLVSSSHNTYLLEDQL 320
TGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRV 400
MARHLRAILGPILLDQPLDGVTTSLPSPEQLKGKILLKGKKLGGLLPAGGENGSEATDVSDEVEAAEMEDEAVRSQVQHK 480
PKEDKLKLVPELSDMIIYCKSVHFGGFSSPGTSGQAFYEMASFSESRALRLLQESGNGFVRHNVSCLSRIYPAGWRTDSS 560
NYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPNTTFNSRALTQGPWWRPERLRVRIISGQQ 640
LPKVNKNKNSIVDPKVIVEIHGVGRDTGSRQTAVITNNGFNPRWDMEFEFEVTVPDLALVRFMVEDYDSSSKNDFIGQST 720
IPWNSLKQGYRHVHLLSKNGDQHPSATLFVKISIQD
................................................................................ 80
................................................................................ 160
...aMaaaMaaMaMaaa............................................................... 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
....................................
//
NES-accession: NES-0025
Date: 14 Aug 2002
Protein: E1B protein, large T-antigen (E1B-55K)
Organism: Human adenovirus type 5
DB_reference: SWISS-PROT P03243, E1BL_ADE05
Necessity: L83A, L87A and L91A abrogates export of full-length protein.
Sufficiency: Aa 83-93 mediates export of GST-GFP fusion.
Pathway: LMB-sensitive.
Location: Cytoplasmic.
Regulation: -
Comments: -
References: Kratzer F, Rosorius O, Heger P, Hirschmann N, Dobner T,
Hauber J and Stauber RH, 2000,
Oncogene 19: 850-7. Medline: 10702793
Sequence: 496 aa
MERRNPSERGVPAGFSGHASVESGCETQESPATVVFRPPGDNTDGGAAAAAGGSQAAAAGAEPMEPESRPGPSGMNVVQV 80
AELYPELRRILTITEDGQGLKGVKRERGACEATEEARNLAFSLMTRHRPECITFQQIKDNCANELDLLAQKYSIEQLTTY 160
WLQPGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAEVEIDTEDRVAFRCSMINMWPGVLGMDGVVIMNVR 240
FTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWTDVRVRGCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNS 320
RVRHNVASDCGCFMLVKSVAVIKHNMVCGNCEDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGNR 400
RGVFLPYQCNLSHTKILLEPESMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGGKHIRNQPVMLDVTEELRPDHLV 480
LACTRAEFGSSDEDTD
................................................................................ 80
..MaaaMaaaMaa................................................................... 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................
//
NES-accession: NES-0026
Date: 14 Aug 2002
Protein: 38 kDa MOV34 ISOLOGUE (Kip1 C-terminus interacting protein-2)
(Jab1/CSN5)
Organism: Mus musculus (Mouse)
DB_reference: TrEMBL O35864
Necessity: L237A/L240A/L241A abrogates export.
Sufficiency: Aa 233-242 is suficient to interact with Crm in vitro,
the interaction being disrupted by L237A/L240A/L241A.
Pathway: LMB-sensitive, Crm-interaction.
Location: Whole cell distribution.
Regulation: -
Comments: -
References: Tomoda K, Kubota Y, Arata Y, Mori S, Maeda M, Tanaka T,
Yoshida M, Yoneda-Kato N and Kato JY, 2002
J Biol Chem 277: 2302-10. Medline: 11704659
Sequence: 334 aa
MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGL 80
MLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQ 160
FQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLEL 240
LWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMS 320
QVIKDKLFNQINVA
................................................................................ 80
................................................................................ 160
........................................................................aaaaMaaM 240
Ma.............................................................................. 320
..............
//
NES-accession: NES-0027
Date: 14 Aug 2002
Protein: Tristetraproline (TTP) (TIS11A protein) (TIS11) (ZFP-36)
Organism: Rattus norvegicus (Rat)
DB_reference: SWISS-PROT P47973, TTP_RAT
Necessity: Delta 1-15 and L3A/I6A/L10A both abrogate export of
aa 1-161.
Sufficiency: Aa 1-101 mediates export of GFP.
Pathway: LMB sensitive.
Location: Whole cell distribution.
Regulation: -
Comments: -
References: Murata T, Yoshino Y, Morita N and Kaneda N, 2002
Biochem Biophys Res Commun 293: 1242-7. Medline: 12054509
Sequence: 320 aa
MDLSAIYESLMSMSHDLSPDHGGTESSGGLWNINSSDSIPSGVTSRLTGRSTSLVEGRSCSWVPPPPGFAPLAPRPGPEL 80
SPSPTSPTATPTTSSRYKTELCRTYSESGRCRYGAKCQFAHGPGELRQANRHPKYKTELCHKFYLQGRCPYGSRCHFIHN 160
PTEDLALPGQPHVLRQSISFSGLPSGRRTSPPPPGFSGPSLSSCSFSPSSSPPPPGDLPLSPSAFSAAPGTPVSRRDPTP 240
ACCPSCRRSTTPSTIWGPLGGLARSPSAHSLGSDPDDYASSGSSLGGSDSPVFEAGVFGPPQPPAPPRRLPIFNRISVSE
aaMaaMaaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 80
aaaaaaaaaaaaaaaaaaaaa........................................................... 160
................................................................................ 240
................................................................................
//
NES-accession: NES-0028
Date: 14 Aug 2002
Protein: NF-kappaB inhibitor alpha
(Major histocompatibility complex enhancer- binding protein MAD3)
(I-kappa-B-alpha) (IkappaBalpha) (IKB-alpha)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P25963, IKBA_HUMAN
Necessity: I52A/L54A abrogates export of full-length I-kappa-B-alpha
fused to GST.
Sufficiency: Aa 22-72 restores rev-delta-NES acitivity (export).
Pathway: LMB-sensitive.
Location: Cytoplasmic.
Regulation: -
Comments: -
References: Johnson C, Van Antwerp D and Hope TJ, 1999
EMBO J 18: 6682-93. Medline: 10581242
Sequence: 317 aa
MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHL 80
AIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASV 160
GVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCG 240
ADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
.....................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaMaaaaaaaaaaaaaaaaaa........ 80
................................................................................ 160
................................................................................ 240
.............................................................................
//
NES-accession: NES-0029
Date: 14 Aug 2002
Protein: Ran-specific GTPase-activating protein
(Ran binding protein 1) (RanBP1)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P43487, RANG_HUMAN
Necessity: L184A/L187A/V189A abrogates export of full-length RanBP1-GFP
Sufficiency: Aa 178-95 can substitute Rev-NES
Pathway: Rev competition (same pathway). Plafker et al. 2000 has
furthermore shown LMB-sensitivity.
Location: Cytoplasmic.
Regulation: -
Comments: Discrepancy of 1 in amino acid numbering between article and
retrieved sequence.
References: Zolotukhin AS and Felber BK, 1997
J Biol Chem 1997 272: 11356-60. Medline: 9111043
Plafker K, Macara IG, 2000
Mol Cell Biol 20: 3510-21. Medline: 10779340
Sequence: 201 aa
MAAAKDTHEDHDTSTENTDESNHDPQFEPIVSLPEQEIKTLEEDEEELFKMRAKLFRFASENDLPEWKERGTGDVKLLKH 80
KEKGAIRLLMRRDKTLKICANHYITPMMELKPNAGSDRAWVWNTHADFADECPKPELLAIRFLNAENAQKFKTKFEECRK 160
EIEEREKKAGSGKNDHAEKVAEKLEALSVKEETKEDAEEKQ
................................................................................ 80
................................................................................ 160
.................aaaaaaMaaMaMaaaaaa......
//
NES-accession: NES-0030
Date: 14 Aug 2002
Protein: Interferon regulatory factor 3 (IRF-3)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q14653, IRF3_HUMAN
Necessity: I139A/L140A/L143A/L144A abrogates export of full-length IRF-3.
Sufficiency: Aa 136-150 mediates export of ovalbumin. Export abrogated by
I139A/L140A/L143A/L144A/M147A/V148A/L149A.
Pathway: LMB-sensitive (data not shown).
Location: Cytoplasmic.
Regulation: Nuclear upon infection with nexcastle disease virus (NDV).
Comments: -
References: Yoneyama M, Suhara W, Fukuhara Y, Fukuda M, Nishida E
and Fujita T, 1998
EMBO J 17: 1087-95. Medline: 9463386
Sequence: 427 aa
MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNF 80
RSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSL 160
AVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPG 240
WPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVF 320
DLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHP 400
LSLTSDQYKAYLQDLVEGMDFQGPGES
................................................................................ 80
.......................................................aaaMMaaMMaaaaaa.......... 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
...........................
//
NES-accession: NES-0031
Date: 14 Aug 2002
Protein: Tumor protein p73 (p53-like transcription factor)
(p53-related protein)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT O15350, P73_HUMAN. NLSdb NLS:270
Necessity: L375A/L377A abrogates export of full-length p73.
Sufficiency: Aa 364-82 mediates export of YFP(2). Export abrogated by
abovementioned mutations.
Pathway: LMB-sensitive.
Location: Nuclear.
Regulation: -
Comments: -
References: Inoue T, Stuart J, Leno R and Maki CG, 2002
J Biol Chem 277: 15053-60. Medline: 11847229
Sequence: 636 aa
MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAA 80
SASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCP 160
IQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPY 240
EPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSA 320
KNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPS 400
HLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSH 480
CTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQL 560
LRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
...........................................aaaaaaaaaaaMaMaaaaa.................. 400
................................................................................ 480
................................................................................ 560
............................................................................
//
NES-accession: NES-0032
Date: 14 Aug 2002
Protein: MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 1 (SMAD 1)
(MOTHERS AGAINST DPP HOMOLOG 1) (MAD-RELATED PROTEIN 1)
(TRANSFORMING GROWTH FACTOR- BETA SIGNALING PROTEIN-1)
(BSP-1) (HSMAD1) (JV4-1)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q15797, SMA1_HUMAN
Necessity: L406A/M409A abrogates export.
Sufficiency: Aa 406-14 mediates export of GST. Export is abrogated by
L406A/M409A.
Pathway: LMB-sensitivity, Crm-dependency.
Location: Whole cell distribution.
Regulation: -
Comments: -
References: Xiao Z, Watson N, Rodriguez C and Lodish HF, 2001
J Biol Chem 276: 39404-10. Medline: 11509558
Sequence: 465 aa
MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHR 80
KGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLG 160
QNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ 240
PMDTNMMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRN 320
STIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGF 400
ETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
.....MaaMaaaaa...................................................
//
NES-accession: NES-0033
Date: 14 Aug 2002
Protein: DUAL SPECIFICITY PROTEIN PHOSPHATASE 1 (MITOGEN-ACTIVATED
PROTEIN KINASE PHOSPHATASE 7) (MAP KINASE PHOSPHATASE 7) (MKP-7)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q9BY84, DUSX_HUMAN
Necessity: L380A/L383A/L385A abrogates export.
Sufficiency: -
Pathway: LMB-sensitive.
Location: Cytoplasmic.
Regulation: -
Comments: -
References: Masuda K, Shima H, Watanabe M and Kikuchi K, 2001,
J Biol Chem 276: 39002-11. Medline: 11489891
Sequence: 665 aa
MAHEMIGTQIVTERLVALLESGTEKVLLIDSRPFVEYNTSHILEAININCSKLMKRRLQQDKVLITELIQHSAKHKVDID 80
CSQKVVVYDQSSQDVASLSSDCFLTVLLGKLEKSFNSVHLLAGGFAEFSRCFPGLCEGKSTLVPTCISQPCLPVANIGPT 160
RILPNLYLGCQRDVLNKELMQQNGIGYVLNASNTCPKPDFIPESHFLRVPVNDSFCEKILPWLDKSVDFIEKAKASNGCV 240
LVHCLAGISRSATIAIAYIMKRMDMSLDEAYRFVKEKRPTISPNFNFLGQLLDYEKKIKNQTGASGPKSKLKLLHLEKPN 320
EPVPAVSEGGQKSETPLSPPCADSATSEAAGQRPVHPASVPSVPSVQPSLLEDSPLVQALSGLHLSADRLEDSNKLKRSF 400
SLDIKSVSYSASMAASLHGFSSSEDALEYYKPSTTLDGTNKLCQFSPVQELSEQTPETSPDKEEASIPKKLQTARPSDSQ 480
SKRLHSVRTSSSGTAQRSLLSPLHRSGSVEDNYHTSFLFGLSTSQQHLTKSAGLGLKGWHSDILAPQTSTPSLTSSWYFA 560
TESSHFYSASAIYGGSASYSAYSCSQLPTCGDQVYSVRRRQKPSDRADSRRSWHEESPFEKQFKRRSCQMEFGESIMSEN 640
RSREELGKVGSQSSFSGSMEIIEVS
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
...........................................................M..M.M............... 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
.........................
//
NES-accession: NES-0034
Date: 14 Aug 2002
Protein: PROTEIN KINASE C, IOTA TYPE (EC 2.7.1.-) (NPKC-IOTA)
(PROTEIN KINASE C LAMBDA)
Organism: Mus musculus (Mouse)
DB_reference: SWISS-PROT Q62074, KPCI_MOUSE
Necessity: F244A/L246A mutant accumulates in the nucleus.
Sufficiency: -
Pathway: LMB-sensitive.
Location: Predominantly cytoplasmic.
Regulation: -
Comments: Discrepancy of 9 in amino acid numbering between article
and retrieved sequence.
References: Perander M, Bjorkoy G and Johansen T, 2001,
J Biol Chem 276: 13015-24. Medline: 11115515
Sequence: 586 aa
MSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKWIDEEGDPCTVSSQLELEEA 80
FRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGY 160
KCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQTMHPDHTQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLG 240
LQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFF 320
VIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTT 400
STFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAA 480
SVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDI 560
VRKIDQSEFEGFEYINPLLMSAEECV
................................................................................ 80
................................................................................ 160
................................................................................ 240
...M.M.......................................................................... 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
..........................
//
NES-accession: NES-0035
Date: 14 Aug 2002
Protein: MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 4 (SMAD 4) (MOTHERS
AGAINST DPP HOMOLOG 4) (DELETION TARGET IN PANCREATIC
CARCINOMA 4) (HSMAD4)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q13485, SMA4_HUMAN
Necessity: L146A/L148A abrogates export.
Sufficiency: -
Pathway: LMB-sensitive.
Location: Cytoplasmic.
Regulation: Export inhibited by protein-protein interactions.
Comments: -
References: Watanabe M, Masuyama N, Fukuda M and Nishida E, 2000
EMBO Rep 1: 176-82. Medline: 11265759
Sequence: 552 aa
MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDG 80
RLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKD 160
EYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQI 240
ASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAP 320
EYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVR 400
CLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAP 480
AISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD
................................................................................ 80
.................................................................M.M............ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
........................................................................
//
NES-accession: NES-0036
Date: 14 Aug 2002
Protein: ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN)
(TRIPARTITE MOTIF PROTEIN 27)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P14373, RFP_HUMAN
Necessity: Aa 132-255 necessary in a deletional mutant context.
L204A/L207A/L209A abrogates export.
Sufficiency: -
Pathway: LMB-sensitive.
Location: -
Regulation: NES activated upon PKC alfa activation, but RFP
is not a direct substrate of the kinase.
Comments: -
References: Harbers M, Nomura T, Ohno S, Ishii S, 2001,
J Biol Chem 276: 48596-607. Medline: 11591718
Sequence: 513 aa
MASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQ 80
LRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRR 160
RAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQ 240
QQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTL 320
DPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTS 400
APQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSG 480
GKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP
................................................................................ 80
................................................................................ 160
...........................................M..M.M............................... 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
.................................
//
NES-accession: NES-0037
Date: 14 Aug 2002
Protein: RPF1/Nedd4, Ubiquitin-protein ligase (E3)
Organism: Homo sapiens (Human)
DB_reference: REFSEQ XP_046129.4
Necessity: Aa 293-309 necessary in a deletional mutant context.
L305A/I307A leads to whole cell distribution.
Sufficiency: -
Pathway: LMB-sensitive.
Location: Cytoplasmic.
Regulation: Constitutive export.
Comments: There is no SWISS-PROT entry matching this sequence:
The swissprot entry P46934 is stated to be only a fragment, but
is longer than the sequence used in the article. The XP_046129
protein though, match the length and residue numbering of the
sequence in the article.
References: Hamilton MH, Tcherepanova I, Huibregtse JM
and McDonnell DP, 2001,
J Biol Chem 276: 26324-31. Medline: 11342538
Sequence: 900 aa
MATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRV 80
HPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDD 160
NAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQA 240
QRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSS 320
NHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQ 400
SQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWE 480
ERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRI 560
MGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGR 640
VAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGS 720
EIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSA 800
NHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFE 880
ELWDKLQMAIENTQGFDGVD
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................M.M............. 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................................................ 880
....................
//
NES-accession: NES-0038
Date: 14 Aug 2002
Protein: TRANSCRIPTIONAL ACTIVATOR PDR4 (YAP-1 PROTEIN) (Yap1p)
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: SWISS-PROT P19880, PDR4_YEAST
Necessity: V616A, L619A and C620T abrogates export, whereas C620A does not.
Sufficiency: -
Pathway: Crm-dependence and interaction with Crm both in vitro and in
vivo.
Location: Cytoplasm.
Regulation: Yap1p responds to oxidative stress. NES masked upon oxidation of
cysteines in the NES region.
Comments: -
References: Yan C, Lee LH and Davis LI, 1998,
EMBO J 17: 7416-29. Medline: 9857197
Sequence: 650 aa
MSVSTAKRSLDVVSPGSLAEFEGSKSRHDEIENEHRRTGTRDGEDSEQPKKKGSKTSKKQDLDPETKQKRTAQNRAAQRA 80
FRERKERKMKELEKKVQSLESIQQQNEVEATFLRDQLITLVNELKKYRPETRNDSKVLEYLARRDPNLHFSKNNVNHSNS 160
EPIDTPNDDIQENVKQKMNFTFQYPLDNDNDNDNSKNVGKQLPSPNDPSHSAPMPINQTQKKLSDATDSSSATLDSLSNS 240
NDVLNNTPNSSTSMDWLDNVIYTNRFVSGDDGSNSKTKNLDSNMFSNDFNFENQFDEQVSEFCSKMNQVCGTRQCPIPKK 320
PISALDKEVFASSSILSSNSPALTNTWESHSNITDNTPANVIATDATKYENSFSGFGRLGFDMSANHYVVNDNSTGSTDS 400
TGSTGNKNKKNNNNSDDVLPFISESPFDMNQVTNFFSPGSTGIGNNAASNTNPSLLQSSKEDIPFINANLAFPDDNSTNI 480
QLQPFSESQSQNKFDYDMFFRDSSKEGNNLFGEFLEDDDDDKKAANMSDDESSLIKNQLINEEPELPKQYLQSVPGNESE 560
ISQKNGSSLQNADKINNGNDNDNDNDVVPSKEGSLLRCSEIWDRITTHPKYSDIDVDGLCSELMAKAKCSERGVVINAED 640
VQLALNKHMN
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
.......................................................M..MM.................... 640
..........
//
NES-accession: NES-0039
Date: 14 Aug 2002
Protein: Putative ATP-dependent RNA helicase An3
Organism: Xenopus laevis (African clawed frog)
DB_reference: SWISS-PROT P24346, AN3_XENLA
Necessity: Delta 1-21 and L19A/L21A both abrogates export.
Sufficiency: -
Pathway: Full-length An3 interacts in vitro with Crm1, whereas delta
1-21 aa does not.
Location: -
Regulation: -
Comments: -
References: Askjaer P, Bachi A, Wilm M, Bischoff FR, Weeks DL, Ogniewski V,
Ohno M, Niehrs C, Kjems J, Mattaj IW, Fornerod M, 1999,
Mol Cell Biol 19: 6276-85. Medline: 10454574
Sequence: 697 aa
MSHVAVENVLNLDQQFAGLDLNSADAESGVAGTKGRYIPPHLRNKEASRNDSNWDSGRGGNGYINGMQDDRDGRMNGYDR 80
GGYGSRGTGRSDRGFYDRENSGWNSGRDKDAYSSFGSRGDRGKGSLFNERGSGSRRTDDRRQDGFDGMGNRSDKSGFGRF 160
DRGNSRWSDDRNDEDDWSKPLAPNDRVEQELFSGSNTGINFEKYDDIPVEATGSNCPPHIESFHDVTMGEIIMGNIQLTR 240
YTRPTPVQKHAIPIIIEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGDAMKHLQENGRYGRRKQFPLSLVLAPTRELA 320
VQIYEEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEP 400
QIRRIVEQDTMPPKGVRQTMMFSATFPKEIQILARDFLDEYIFLAVGRVGSTSENITQKVVWVEEMDKRSFLLDLLNATG 480
KDSLTLVFVETKKGADALEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLP 560
SDIEEYVHRIGRTGRVGNLGLATSFFNEKNINITKDLLDLLVEAKQEVPSWLENMAYEQHHKSSSRGRSKSRFSGGFGAK 640
DYRQSSGAGSSFGSSRGGRSSGHGGSRGFGGGYGGFYNSDGYGGNYGGSSQVDWWGN
..................M.M........................................................... 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
.........................................................
//
NES-accession: NES-0040
Date: 14 Aug 2002
Protein: M-phase inducer phosphatase 1/B (Cdc25)
Organism: Xenopus laevis (African clawed frog)
DB_reference: SWISS-PROT P30309, MPI1_XENLA
Necessity: Both L53A/V55A/F57A and L344A/V347A/I349A greatly impair export
of full-length fused to GFP.
Sufficiency: -
Pathway: LMB-sensitivity.
Location: Cytoplasmic.
Regulation: -
Comments: Aa 47-57 is a stronger NES than aa 342-50.
References: Kumagai A and Dunphy WG, 1999
Genes Dev 13: 1067-72. Medline: 10323858
Sequence: 550 aa
MAESHIMSSEAPPKTNTGLNFRTNCRMVLNLLREKDCSVTFSPEQPLTPVTDLAVGFSNLSTFSGETPKRCLDLSNLGDE 80
TAPLPTESPDRISSGKVESPKAQFVQFDGLFTPDLGWKAKKCPRGNMNSVLPRLLCSTPSFKKTSGGQRSVSNKENEGEL 160
FKSPNCKPVALLLPQEVVDSQFSPTPENKVDISLDEDCEMNILGSPISADPPCLDGAHDDIKMQNLDGFADFFSVDEEEM 240
ENPPGAVGNLSSSMAILLSGPLLNQDIEVSNVNNISLNRSRLYRSPSMPEKLDRPMLKRPVRPLDSETPVRVKRRRSTSS 320
SLQPQEENFQPQRRGTSLKKTLSLCDVDISTVLDEDCGHRQLIGDFTKVYALPTVTGRHQDLRYITGETLAALIHGDFSS 400
LVEKIFIIDCRYPYEYDGGHIKGALNLHRQEEVTDYFLKQPLTPTMAQKRLIIIFHCEFSSERGPKMCRFLREEDRARNE 480
YPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPMHHQDFREELLKFRTKCKTSVGDRKRREQIARIMKL
....................................................M.M.M....................... 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
.......................M..M.M................................................... 400
................................................................................ 480
......................................................................
//
NES-accession: NES-0041
Date: 14 Aug 2002
Protein: Adenomatous polyposis coli protein (APC protein)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P25054, APC_HUMAN
Necessity: Export of either full-length APC or a VP16-NLS-aa1-270 fusion
protein, both get abrogated by simultaneous mutation of both
NESs (L75A/L77A/L172A/L174A).
Sufficiency: Aa 68-77 and 165-74 are each sufficient to mediate
export of GST, aa 68-77 being a stronger NES than 165-74.
Pathway: LMB-sensitivity: In vivo interaction with Crm is dependent
on the intact NES sequences.
Location: Cytoplasmic.
Regulation: -
Comments: 2 NESs.
References: Neufeld KL, Nix DA, Bogerd H, Kang Y, Beckerle MC,
Cullen BR and White RL, 2000,
Proc Natl Acad Sci U S A 97: 12085-90. Medline: 11035805
Sequence: 2843 aa
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSS 80
NFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYA 160
QLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQAT 240
EAERSSQNKHETGSHDAERQNEGQGVGEINMATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLS 320
MLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRG 400
RREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQ 480
VDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSK 560
KTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILR 640
NVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAM 720
GSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQSLYGDYV 800
FDTNRHDDNRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLGNYHPATENPGTSSKRGLQI 880
STTAAQIAKVMEEVSAIHTSQEDRSSGSTTELHCVTDERNALRRSSAAHTHSNTYNFTKSENSNRTCSMPYAKLEYKRSS 960
NDSLNSVSSSDGYGKRGQMKPSIESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGR 1040
QSPSQNERWARPKHIIEDEIKQSEQRQSRNQSTTYPVYTESTDDKHLKFQPHFGQQECVSPYRSRGANGSETNRVGSNHG 1120
INQNVSQSLCQEDDYEDDKPTNYSERYSEEEQHEEEERPTNYSIKYNEEKRHVDQPIDYSLKYATDIPSSQKQSFSFSKS 1200
SSGQSSKTEHMSSSSENTSTPSSNAKRQNQLHPSSAQSRSGQPQKAATCKVSSINQETIQTYCVEDTPICFSRCSSLSSL 1280
SSAEDEIGCNQTTQEADSANTLQIAEIKEKIGTRSAEDPVSEVPAVSQHPRTKSSRLQGSSLSSESARHKAVEFSSGAKS 1360
PSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPP 1440
PPPQTAQTKREVPKNKAPTAEKRESGPKQAAVNAAVQRVQVLPDADTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDV 1520
ELRIMPPVQENDNGNETESEQPKESNENQEKEAEKTIDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKPAQTASK 1600
LPPPVARKPSQLPVYKLLPSQNRLQPQKHVSFTPGDDMPRVYCVEGTPINFSTATSLSDLTIESPPNELAAGEGVRGGAQ 1680
SGEFEKRDTIPTEGRSTDEAQGGKTSSVTIPELDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASASSSAP 1760
NKNQLDGKKKKPTSPVKPIPQNTEYRTRVRKNADSKNNLNAERVFSDNKDSKKQNLKNNSKDFNDKLPNNEDRVRGSFAF 1840
DSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKAKENKESEAKVTSHTELTSNQQSANKTQAIAKQPINR 1920
GQPKPILQKQSTFPQSSKDIPDRGAATDEKLQNFAIENTPVCFSHNSSLSSLSDIDQENNNKENEPIKETEPPDSQGEPS 2000
KPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKPSRLKGDNEKHSPRNMGGILGEDLTLDL 2080
KDIQRPDSEHGLSPDSENFDWKAIQEGANSIVSSLHQAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFT 2160
SNKGPRILKPGEKSTLETKKIESESKGIKGGKKVYKSLITGKVRSNSEISGQMKQPLQANMPSISRGRTMIHIPGVRNSS 2240
SSTSPVSKKGPPLKTPASKSPSEGQTATTSPRGAKPSVKSELSPVARQTSQIGGSSKAPSRSGSRDSTPSRPAQQPLSRP 2320
IQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQMSQQNLTKQTGLSKNASSIPRSESASKG 2400
LNQMNNGNGANKKVELSRMSSTKSSGSESDRSERPVLVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPASPTRSQAQ 2480
TPVLSPSLPDMSLSTHSSVQAGGWRKLPPNLSPTIEYNDGRPAKRHDIARSHSESPSRLPINRSGTWKREHSKHSSSLPR 2560
VSTWRRTGSSSSILSASSESSEKAKSEDEKHVNSISGTKQSKENQVSAKGTWRKIKENEFSPTNSTSQTVSSGATNGAES 2640
KTLIYQMAPAVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKANPNIKDSKDNQAKQNVGNGSVPMRTVGLEN 2720
RLNSFIQVDAPDQKGTEIKPGQNNPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSA 2800
RPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHSGSYLVTSV
...................................................................aaaaaaaMaM... 80
................................................................................ 160
....aaaaaaaMaM.................................................................. 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................................................ 880
................................................................................ 960
................................................................................ 1040
................................................................................ 1120
................................................................................ 1200
................................................................................ 1280
................................................................................ 1360
................................................................................ 1440
................................................................................ 1520
................................................................................ 1600
................................................................................ 1680
................................................................................ 1760
................................................................................ 1840
................................................................................ 1920
................................................................................ 2000
................................................................................ 2080
................................................................................ 2160
................................................................................ 2240
................................................................................ 2320
................................................................................ 2400
................................................................................ 2480
................................................................................ 2560
................................................................................ 2640
................................................................................ 2720
................................................................................ 2800
...........................................
//
NES-accession: NES-0042
Date: 14 Aug 2002
Protein: Phosphorylated adaptor for RNA export (PHAX)
Organism: Mus musculus (Mouse)
DB_reference: TrEMBL Q9JJT9
Necessity: V120A/L124A/L127A/M129A prevents Crm-association.
Sufficiency: -
Pathway: Crm-complex formation.
Location: -
Regulation: Exported upon phosphorylation.
Comments: -
References: Ohno M, Segref A, Bachi A, Wilm M and Mattaj IW, 2000,
Cell 101: 187-98. Medline: 10786834
Sequence: 385 aa
MALEAGDMEEGQLSDSDSDMTVVPSDRPLQMAKVLGGGSAACAPVSHYRTVKHVDSSEESLDSDDDCSLWKRKRQKCHNT 80
PPKPEPFPFGPSGQKTALNGGKKVNNIWGAVLQEQNQDAVATELGILGMEGSIDRSRQSETYNYLLAKKLAKKESQEYTK 160
ELDKDLDEYMHGDKKPGSKEDENGQGHLKRKRPVRDRLGNRVEMNYKGRYEITEEDAPEKVADEIAFRLQEPKKDLIARV 240
VRILGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYVENQKEYENKKAARKRRTQL 320
LGKKMKQAIKSLNFQEDDDTSRETFASDTNEALASLDEAQEGPGETKLDAEEAIEVDHPQDLDIF
................................................................................ 80
.......................................M...M..M.M............................... 160
................................................................................ 240
................................................................................ 320
.................................................................
//
NES-accession: NES-0043
Date: 14 Aug 2002
Protein: Brain expressed X-linked protein 3
(P75NTR-associated cell death EXECUTOR) (NADE)
Organism: Mus musculus (Mouse)
DB_reference: TrEMBL Q9WTZ9
Necessity: L94A/L97A/L99A leads to whole cell distribution.
Sufficiency: -
Pathway: LMB-sensitive.
Location: Cytoplasmic.
Regulation: -
Comments: -
References: Mukai J, Shoji S, Kimura MT, Okubo S, Sano H, Suvanto P,
Li Y, Irie S and Sato TA, 2002,
J Biol Chem 277: 13973-82. Medline: 11830582
Sequence: 124 aa
MANVHQENEEMEQPLQNGQEDRPVGGGEGHQPAANNNNNNHNHNHNHHRRGQARRLAPNFRWAIPNRQMNDGLGGDGDDM 80
EMFMEEMREIRRKLRELQLRNCLRILMGELSNHHDHHDEFCLMP
................................................................................ 80
.............M..M.M.........................
//
NES-accession: NES-0044
Date: 14 Aug 2002
Protein: Open reading frame YDR499w (Cip1p)
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: REFSEQ NP_010787.1
Necessity: L670A/L673A abrogates export
Sufficiency: Aa 660-747 fragment interacts with Crm in vitro, in a
LMB-sensitive manner.
Pathway: LMB-sensitive.
Location: Whole cell distribution.
Regulation: -
Comments: Sequence stated as open reading frame YDR499w by the
authors which corresponds to REFSEQ NP_010787.1.
No corresponding SWISS-PROT entry.
References: Jensen TH, Neville M, Rain JC, McCarthy T, Legrain P
and Rosbash M, 2000,
Mol Cell Biol 20: 8047-58. Medline: 11027275
Sequence: 747 aa
MRRETVGEFSSDDDDDILLELGTRPPRFTQIPPSSAALQTQIPTTLEVTTTTLNNKQSKNDNQLVNQLNKAQGEASMLRD 80
KINFLNIEREKEKNIQAVKVNELQVKHLQELAKLKQELQKLEDEKKFLQMEARGKSKREVITNVKPPSTTLSTNTNTITP 160
DSSSVAIEAKPQSPQSKKRKISDNLLKKNMVPLNPNRIIPDETSLFLESILLHQIIGADLSTIEILNRLKLDYITEFKFK 240
NFVIAKGAPIGKSIVSLLLRCKKTLTLDRFIDTLLEDIAVLIKEISVHPNESKLAVPFLVALMYQIVQFRPSATHNLALK 320
DCFLFICDLIRIYHHVLKVPIHESNMNLHVEPQIFQYELIDYLIISYSFDLLEGILRVLQSHPKQTYMEFFDENILKSFE 400
FVYKLALTISYKPMVNVIFSAVEVVNIITSIILNMDNSSDLKSLISGSWWRDCITRLYALLEKEIKSGDVYNENVDTTTL 480
HMSKYHDFFGLIRNIGDNELGGLISKLIYTDRLQSVPRVISKEDIGMDSDKFTAPIIGYKMEKWLLKLKDEVLNIFENLL 560
MIYGDDATIVNGEMLIHSSKFLSREQALMIERYVGQDSPNLDLRCHLIEHTLTIIYRLWKDHFKQLREEQIKQVESQLIM 640
SLWRFLVCQTETVTANEREMRDHRHLVDSLHDLTIKDQASYYEDAFEDLPEYIEEELKMQLNKRTGRIMQVKYDEKFQEM 720
ARTILESKSFDLTTLEEADSLYISMGL
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
...................aaaaaaaaaaMaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 720
aaaaaaaaaaaaaaaaaaaaaaaaaaa
//
NES-accession: NES-0045Date: 14 Aug 2002
Protein: Nonstructural protein NS2
Organism: Influenza B virus (strain B/Yamagata/1/73)
DB_reference: SWISS-PROT P08014, VNS2_INBYA
Necessity: NEP-B can substitute for HIV1 Rev effector domain, whereas
M14A/M17A/I19A cannot.
Sufficiency: -
Pathway: Crm-interaction in 2 hybrid system
Location: -
Regulation: -
Comments: -
References: Paragas J, Talon J, O'Neill RE, Anderson DK, Garcia-Sastre A
and Palese P, 2001,
J Virol 75: 7375-83. Medline: 11462009
Sequence: 122 aa
MADNMTTTQIEWRMKKMAIGSSTHSSSVLMKDIQSQFEQLKLRWESYPNLVKSTDYHQRRETIRLVTEELYLLSKRIDDN 80
ILFHKTVIANSSIIADMIVSLSLLETLYEMKDVVEVYSRQCL
.............M..M.M............................................................. 80
..........................................
//
NES-accession: NES-0046
Date: 14 Aug 2002
Protein: Nonstructural protein NS2
Organism: Influenza A virus (strain A/Puerto Rico/8/34)
DB_reference: SWISS-PROT P03508, VNS2_IAPUE
Necessity: Fragments containg aa 1-30 can substitute for HIV1 Rev effector
domain, whereas fragments lacking aa 1-30 cannot.
M16A/M19A/L21A in full-length context abrogates export activity.
Sufficiency: Aa 1-30 can substitue for HIV1 Rev effector domain.
Aa 11-23 mediates export of GST, but if containg the above-
mentioned mutations, export is abrogated.
Pathway: -
Location: -
Regulation: -
Comments: -
References: O'Neill RE, Talon J and Palese P, 1998,
EMBO J 17: 288-96. Medline: 9427762
Sequence: 121 aa
MDPNTVSSFQDILLRMSKMQLESSSGDLNGMITQFESLKLYRDSLGEAVMRMGDLHSLQNRNEKWREQLGQKFEEIRWLI 80
EEVRHKLKITENSFEQITFMQALHLLLEVEQEIRTFSFQLI
...............M..M.M........................................................... 80
.........................................
//
NES-accession: NES-0047
Date: 14 Aug 2002
Protein: NF-kappaB inhibitor epsilon (NF-kappa-BIE) (I-kappa-B-epsilon)
(IkappaBepsilon) (IKB-epsilon) (IKBE)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT O00221, IKBE_HUMAN
Necessity: L350A/I352A abrogates export of full-length IKBE.
Sufficiency: -
Pathway: LMB-sensitive, Crm-interaction in vitro.
Location: Cytoplasmic.
Regulation: -
Comments: -
References: Lee SH and Hannink M, 2002,
J Biol Chem 277: 23358-66. Medline: 11970947
Sequence: 361 aa
MSEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASGPSDGSPQPCTHPPGPVKEPQEKEDADGERADSTYGSSSLTY 80
TLSLLGGPEAEDPPPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCCLALLPQEVLDIQNNLYQTALH 160
LAVHLDQPGAVRALVLKGASRALQDRHGDTALHVACQRQSWPVPAACWKGGPEPGRGTSHSLDLQLQNWQGLACLHIATL 240
QKNQPLMELLLRNGADIDVQEGSSGKTALHLAVETQERGLVQFLLQAGAQVDARMLNGCTPLHLAAGRGLMGISSTLCKA 320
GADSLLRNVEDETPQDLTEESLVLLPFDDLKISGKLLLCTD
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
.............................M.M.........
//
NES-accession: NES-0048
Date: 14 Aug 2002
Protein: ATP-dependent RNA helicase p54 (Xp54) (DDX6)
Organism: Xenopus laevis (African clawed frog)
DB_reference: SWISS-PROT P54824, DDX6_XENLA
Necessity: L157A/L159A abrogates export of aa 1-164 fragment.
Sufficiency: Aa 1-164 mediates export of GFP.
Pathway: LMB-sensitive.
Location: Cytoplasmic in quiescent and full-grown oocytes, but nuclear
in developing oocytes.
Regulation: -
Comments: Discrepancy of 1 in amino acid numbering between article and
retrieved sequence.
References: Smillie DA and Sommerville J, 2001,
J Cell Sci115: 395-407. Medline: 11839790
Sequence: 481 aa
MSTARTENPVLMGMSSQNGQLRGPLKPSAGPGGGGTQTQQINQLKNASTINSGSQQQAQSMSSIIKPGDDWKKTLKLPPK 80
DLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKTGAYLIPLLERLDLK 160
KDCIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHIQ 240
MIVLDEADKLLSQDFMQIMEDIIMTLPKNRQILLYSATFPLSVQKFMTLHLQKPYEINLMEELTLKGVTQYYAYVTERQK 320
VHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQ 400
AVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSSIDKNLYVAEYHSESGEDK 480
P
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 80
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaMa 160
aaaa............................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
.
//
NES-accession: NES-0049
Date: 14 Aug 2002
Protein: TELOMERASE REVERSE TRANSCRIPTASE (TELOMERASE CATALYTIC SUBUNIT)
(HEST2) (TERT)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT O14746, TERT_HUMAN
Necessity: -
Sufficiency: In vitro Crm-interaction of aa 831-1132 fused to GST was
abrogated by L974A/L978A/L980A.
Pathway: LMB-sensitive.
Location: Cytoplasmic in the absence of 14-3-3, nuclear in the
presence of 14-3-3.
Regulation: NES masked upon binding of 14-3-3 protein.
Comments: -
References: Seimiya H, Sawada H, Muramatsu Y, Shimizu M, Ohko K,
Yamane K and Tsuruo T, 2000,
EMBO J 2000 19: 2652-61. Medline: 10835362
Sequence: 1132 aa
MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKEL 80
VARVLQRLCERGAKNVLAFGFALLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFV 160
LVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARRRGGSASRSLPLPKRPRR 240
GAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTP 320
CPPVYAETKHFLYSSGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNH 400
AQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPWQVYGFVRACLRRLVPPGLWGS 480
RHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSF 560
FYVTETTFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNMDYVV 640
GARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTI 720
PQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNE 800
ASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLLVTPHLTHA 880
KTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTF 960
NRGFKAGRNMRRKLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTA 1040
SLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLPGTTLTALEAAAN 1120
PALPSDFKTILD
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
..............................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 880
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 960
aaaaaaaaaaaaaMaaaMaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 1040
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 1120
aaaaaaaaaaaa
//
NES-accession: NES-0050
Date: 14 Aug 2002
Protein: Ran GTPase activating protein 1 (Rna1p)
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: SWISS-PROT P11745, RNA1_YEAST
Necessity: -
Sufficiency: Aa 316-57 mediates export of H2B-lacZ fusion.
Aa 316-33 and 329-57 each mediates export, aa 329-57 being most
effective.
L326A/I328A abrogates export of aa 316-33 fragment.
Pathway: Crm-dependent
Location: Cytoplasmic
Regulation: -
Comments: 2 overlapping NESs. "S" and "E" in the assignment field,
describes start and end of fragments shown to have NES activity.
References: Feng W, Benko AL, Lee JH, Stanford DR and Hopper AK, 1999,
J Cell Sci 112: 339-47. Medline: 9885287
Sequence: 407 aa
MATLHFVPQHEEEQVYSISGKALKLTTSDDIKPYLEELAALKTCTKLDLSGNTIGTEASEALAKCIAENTQVRESLVEVN 80
FADLYTSRLVDEVVDSLKFLLPVLLKCPHLEIVNLSDNAFGLRTIELLEDYIAHAVNIKHLILSNNGMGPFAGERIGKAL 160
FHLAQNKKAASKPFLETFICGRNRLENGSAVYLALGLKSHSEGLKVVKLYQNGIRPKGVATLIHYGLQYLKNLEILDLQD 240
NTFTKHASLILAKALPTWKDSLFELNLNDCLLKTAGSDEVFKVFTEVKFPNLHVLKFEYNEMAQETIEVSFLPAMEKGNL 320
PELEKLEINGNRLDEDSDALDLLQSKFDDLEVDDFEEVDSEDEEGEDEEDEDEDEKLEEIETERLEKELLEVQVDDLAER 400
LAETEIK
................................................................................ 80
................................................................................ 160
................................................................................ 240
...........................................................................Saaaa 320
aaaaaMaMSaaaEaaaaaaaaaaaaaaaaaaaaaaaE........................................... 400
.......
//
NES-accession: NES-0051
Date: 14 Aug 2002
Protein: Transcription factor IIIA (Factor A) (TFIIIA)
Organism: Bufo americanus (American toad)
DB_reference: SWISS-PROT P34694, TF3A_BUFAM
Necessity: -
Sufficiency: Aa 320-339 mediates export of BSA in oocytes and of GST in HeLa
cells. Export abrogated by L336A.
Pathway: Aa 320-339 can substitute for the HIV-1 Rev effector domain.
Location: -
Regulation: -
Comments: -
References: Fridell RA, Fischer U, Luhrmann R, Meyer BE, Meinkoth JL,
Malim MH and Cullen BR, 1996,
Proc Natl Acad Sci U S A 93: 2936-40. Medline: 8610146
Sequence: 339 aa
MGEKLPVVYKRFICSFPDCNATYNKNRKLQAHLCKHTGERPFPCTYEGCEKGFVTLHHLNRHVLSHTGEKPCKCETENCN 80
LAFTTASNMRLHFKRAHSSPAQVYVCYFADCGQQFRKHNQLKIHQYIHTNQQPFKCSHEGCDKCYASPSRLKRHEKTHAG 160
YPCRKDSTCPFVGKTWSDYMKHAAELHSEVTCSICNRTFKRKSFLKEHKKIHREERIVYRCPRENCDRTYTTKFNLKSHI 240
LTFHENLRPFVCEHEGCGKTFAMKQSLDRHFNTHDPEKKKMVKPPRPVRSLASRLSGYKPKKSKKKKKPSQTPAMESQEQ 320
QPDASKADPLPVLENLTLK
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
aaaaaaaaaaaaaaaMaaa
//
NES-accession: NES-0052
Date: 14 Aug 2002
Protein: Transcription factor IIIA (Factor A) (TFIIIA) (S-TFIIIA/O-TFIIIA)
Organism: Xenopus laevis (African clawed frog)
DB_reference: SWISS-PROT P03001, TF3A_XENLA
Necessity: -
Sufficiency: Aa 347-66 mediates export of reporterprotein.
Pathway: Aa 347-66 can complement for the HIV-1 Rev effector
domain.
Location: -
Regulation: -
Comments: -
References: Fridell RA, Fischer U, Luhrmann R, Meyer BE, Meinkoth JL,
Malim MH and Cullen BR, 1996,
Proc Natl Acad Sci U S A 93: 2936-40. Medline: 8610146
Ward BM and Lazarowitz SG, 1999,
Plant Cell 11: 1267-76. Medline: 10402428
Sequence: 366 aa
MAAKVASTSSEEAEGSLVTEGEMGEKALPVVYKRYICSFADCGAAYNKNWKLQAHLCKHTGEKPFPCKEEGCEKGFTSLH 80
HLTRHSLTHTGEKNFTCDSDGCDLRFTTKANMKKHFNRFHNIKICVYVCHFENCGKAFKKHNQLKVHQFSHTQQLPYECP 160
HEGCDKRFSLPSRLKRHEKVHAGYPCKKDDSCSFVGKTWTLYLKHVAECHQDLAVCDVCNRKFRHKDYLRDHQKTHEKER 240
TVYLCPRDGCDRSYTTAFNLRSHIQSFHEEQRPFVCEHAGCGKCFAMKKSLERHSVVHDPEKRKLKEKCPRPKRSLASRL 320
TGYIPPKSKEKNASVSGTEKTDSLVKNKPSGTETNGSLVLDKLTIQ
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
..........................aaaaaaaaaaaaaaaaaaaa
//
NES-accession: NES-0053
Date: 14 Aug 2002
Protein: Ah receptor (Aryl hydrocarbon receptor) (AhR)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P35869, AHR_HUMAN
Necessity: -
Sufficiency: Aa 55-75 mediates export of GFP-GST fusion. Abrogated by
L70A/L72A.
Pathway: Export abrogated by competition of the PKI-NES (NES-0001), which
indicates that AhR gets exported by the same pathway as PKI.
Location: -
Regulation: -
Comments: -
References: Ikuta T, Eguchi H, Tachibana T, Yoneda Y and Kawajiri K, 1998
J Biol Chem 273: 2895-904. Medline: 9446600
Sequence: 848 aa
MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAK 80
SFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYE 160
LIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQ 240
GKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGS 320
GYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLTDEEGTEHLRKRNT 400
KLPFMFTTGEAVLYEATNPFPAIMDPLPLRTKNGTSGKDSATTSTLSKDSLNPSSLLAAMMQQDESIYLYPASSTSSTAP 480
FENNFFNESMNECRNWQDNTAPMGNDTILKHEQIDQPQDVNSFAGGHPGLFQDSKNSDLYSIMKNLGIDFEDIRHMQNEK 560
FFRNDFSGEVDFRDIDLTDEILTYVQDSLSKSPFIPSDYQQQQSLALNSSCMVQEHLHLEQQQQHHQKQVVVEPQQQLCQ 640
KMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYP 720
MGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLN 800
KFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL
......................................................aaaaaaaaaaaaaaaMaMaaa..... 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................
//
NES-accession: NES-0054
Date: 14 Aug 2002
Protein: Period circadian protein 2 (mPER2)
Organism: Mus musculus (Mouse)
DB_reference: SWISS-PROT O54943, PER2_MOUSE
Necessity: -
Sufficiency: Aa 382-469 mediates export of YFP(2)-NLS. Export is abrogated
by I464A/L467A/M469K and greatly impaired by M469K alone.
Pathway: LMB-sensitive.
Location: -
Regulation: -
Comments: -
References: Vielhaber EL, Duricka D, Ullman KS and Virshup DM, 2001
J Biol Chem 276: 45921-7. Medline: 11591712
Sequence: 1257 aa
MNGYVDFSPSPTSPTKEPGAPQPTQAVLQEDVDMSSGSSGNENCSTGRDSQGSDCDDNGKELRMLVESSNTHPSPDDAFR 80
LMMTEAEHNPSTSGCSSEQSAKADAHKELIRTLKELKVHLPADKKAKGKASTLATLKYALRSVKQVKANEEYYQLLMSSE 160
SQPCSVDVPSYSMEQVEGITSEYIVKNADMFAVAVSLVSGKILYISNQVASIFHCKKDAFSDAKFVEFLAPHDVSVFHSY 240
TTPYKLPPWSVCSGLDSFTQECMEEKSFFCRVSVGKHHENEIRYQPFRMTPYLVKVQEQQGAESQLCCLLLAERVHSGYE 320
APRIPPEKRIFTTTHTPNCLFQAVDERAVPLLGYLPQDLIETPVLVQLHPSDRPLMLAIHKKILQAGGQPFDYSPIRFRT 400
RNGEYITLDTSWSSFINPWSRKISFIIGRHKVRVGPLNEDVFAAPPCPEEKTPHPSVQELTEQIHRLLMQPVPHSGSSGY 480
GSLGSNGSHEHLMSQTSSSDSNGQEESHRRRSGIFKTSGKIQTKSHVSHESGGQKEASVAEMQSSPPAQVKAVTTIERDS 560
SGASLPKASFPEELAYKNQPPCSYQQISCLDSVIRYLESCSEAATLKRKCEFPANIPSRKATVSPGLHSGEAARPSKVTS 640
HTEVSAHLSSLTLPGKAESVVSLTSQCSYSSTIVHVGDKKPQPELETVEDMASGPESLDGAAGGLSQEKGPLQKLGLTKE 720
VLAAHTQKEEQGFLQRFREVSRLSALQAHCQNYLQERSRAQASDRGLRNTSGLESSWKKTGKNRKLKSKRVKTRDSSEST 800
GSGGPVSHRPPLMGLNATAWSPSDTSQSSCPSAPFPTAVPAYPLPVFQAPGIVSTPGTVVAPPAATHTGFTMPVVPMGTQ 880
PEFAVQPLPFAAPLAPVMAFMLPSYPFPPATPNLPQAFLPSQPHFPAHPTLASEITPASQAEFPSRTSTLRQPCACPVTP 960
PAGTVALGRASPPLFQSRGSSPLQLNLLQLEEAPEGSTGAAGTLGTTGTAASGLDCTSGTSRDRQPKAPPTCNEPSDTQN 1040
SDAISTSSDLLNLLLGEDLCSATGSALSRSGASATSDSLGSSSLGFGTSQSGAGSSDTSHTSKYFGSIDSSENNHKAKMI 1120
PDTEESEQFIKYVLQDPIWLLMANTDDSIMMTYQLPSRDLQAVLKEDQEKLKLLQRSQPRFTEGQRRELREVHPWVHTGG 1200
LPTAIDVTGCVYCESEEKGNICLPYEEDSPSPGLCDTSEAKEEEGEQLTGPRIEAQT
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
.............................................................aaaaaaaaaaaaaaaaaaa 400
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaMaM........... 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................................................ 880
................................................................................ 960
................................................................................ 1040
................................................................................ 1120
................................................................................ 1200
.........................................................
//
NES-accession: NES-0055
Date: 14 Aug 2002
Protein: Nucleoprotein p40 (N protein)
Organism: Borna disease virus (BDV)
DB_reference: TrEMBL Q91UL8
Necessity: -
Sufficiency: Aa 128-41 mediates export of GFP. L128A/L131A/I133A/I136A
abrogates export.
Pathway: LMB-sensitive.
Location: -
Regulation: BDV P protein masks the NES of p38 N protein, and prevent its
export.
Comments: This is the sequence of p40, p38 might be more correct.This
sequence fits with the aa-numbering in the paper though.
References: Kobayashi T, Kamitani W, Zhang G, Watanabe M, Tomonaga K
and Ikuta K, 2001,
J Virol 75: 3404-12. Medline: 11238866
Sequence: 370 aa
MPPKRRLVDDADAMEDQDLYEPPASLPKLPGKFLQYTVGGSDPHPGIGHEKDIRQNAVALLDQSRRDMFHTVTPSLVFLC 80
LLIPGLHAAFVHGGVPRESYLSTPVTRGEQTVVKTAKFYGEKTTQRDLTELEISSIFSHCCSLLIGVVIGSSSKIKAGAE 160
QIKKRFKTMMAALNRPSHGETATLLQMFNPHEAIDWINGQPWVGSFVLSLLTTDFESPGKEFMDQIKLVASYAQMTTYTT 240
IKEYLAECMDATLTIPVVAYEIRDFLEVSAKLKEEHADLFPFLGAIRHPDAIKLAPRSFPNLASAAFYWSKKENPTMAGY 320
RASTIQPGASVKETQLARYRRREISRGEDGAELSGEISAIMKMIGVTGLN
................................................................................ 80
...............................................MaaMaMaaMaaaaa................... 160
................................................................................ 240
................................................................................ 320
..................................................
//
NES-accession: NES-0056
Date: 14 Aug 2002
Protein: Zyxin
Organism: Gallus gallus (Chicken)
DB_reference: SWISS-PROT Q04584, ZYX_CHICK
Necessity: Delta 322-31 abrogates export of full-length Zyxin.
Sufficiency: Aa 319-35 mediates export of GST, whereas aa 322-31 does not.
Pathway: -
Location: Colocalizes with integrin receptors at focal contacts.
Regulation: -
Comments: -
References: Nix DA and Beckerle MC, 1997,
J Cell Biol 1138: 1139-47. Medline: 9281590
Sequence: 542 aa
MASPGTPGTRMTTTVSINISTPSFYNPQKKFAPVVAPKPKVNPFKTGGTSESSQPQPPGTGAQRAQIGRVGEIPVSVTAE 80
ELPLPPPPPPGEELSFSSNCAFPPPPPPFEEPFPPAPDEAFPSPPPPPPPMFDEGPALQIPPGSTGSVEKPLAPKAHVEI 160
SSAPRDPTPPFPSKFTPKPSGTLSSKPPGLDSTPAPAPWAAPQQRKEPLASVPPPPSLPSQPTAKFTPPPVASSPGSKPG 240
ATVPMAPSNSTRYPTSLQTQFTAPSPSGPLSRPQPPNFTYAQQWERPQVQEKPVPTEKSAAVKDMRRPTADPPKGNSPLT 320
MKEVEELELLTQKLMKDMDHPPPVEAATSELCGFCRKPLSRTQPAVRALDCLFHVECFTCFKCEKQLQGQQFYNVDEKPF 400
CEDCYAGTLEKCSVCKQTITDRMLKATGNSYHPQCFTCVMCHTPLEGASFIVDQANQPHCVDDYHRKYAPRCSVCSEPIM 480
PEPGKDETVRVVALEKNFHMKCYKCEDCGRPLSIEADENGCFPLDGHVLCMKCHTVRAKTAC
................................................................................ 80
................................................................................ 160
................................................................................ 240
...............................................................................a 320
aaaaaaaaaaaaaaa................................................................. 400
................................................................................ 480
..............................................................
//
NES-accession: NES-0057
Date: 14 Aug 2002
Protein: Eukaryotic translation initiation factor 3 subunit 6 (eIF-3 p48)
(Mammary tumor-associated protein INT-6) (Viral integration site
protein INT-6)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q64252, IF36_HUMAN
Necessity: Aa 1-10 necessary in a deletional mutant context.
Sufficiency: Aa 1-20 mediates export of the nuclear protein DRBP76.
Export abrogated by either L6A, L14A or L18A.
Pathway: -
Location: Cytoplasmic.
Regulation: -
Comments: -
References: Guo J and Sen GC, 2000,
J Virol 74: 1892-9. Medline: 10644362
Sequence: 445 aa
MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKNLYSDDIPHALREKRTTVVAQ 80
LKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADKHGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPA 160
TDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQP 240
QYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLV 320
NDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSP 400
YQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY
aaaaaMaaaaaaaMaaaMaa............................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
.............................................
//
NES-accession: NES-0058
Date: 14 Aug 2002
Protein: 52 kDa immediate-early phosphoprotein (ORF 57)
Organism: Herpesvirus saimiri (strain 11).
DB_reference: SWISS-PROT P13199, IE63_HSVSA
Necessity: L164A/L166A abrogates export of full-length protein.
Sufficiency: Aa 154-170 mediates export of GFP.
Pathway: -
Location: -
Regulation: -
Comments: -
References: Goodwin DJ, Hall KT, Stevenson AJ, Markham AF
and Whitehouse A, 1999,
J Virol 73: 10519-24. Medline: 10559371
Sequence: 417 aa
MEDIIEGGISSDDDFDSSDSSSDEEESDTSPQIMKSDVTMASPPSTPEPSPDVSASTSNLKRERQRSPITWEHQSPLSRV 80
YRSPSPMRFGKRPRISSNSTSRSCKTSWADRVREAAAQRRPSRPFRKPYSHPRNGPLRNGPPRAPPLLKLFDISILPKSG 160
EPKLFLPVPSLPCQEAEKTNDKYVLAMAQRAMHDVPISSKQLTANLLPVKFKPLLSIVRYTPNYYYWVSMRKETIASANL 240
CTVAAFLDESLCWGQQYLKNDFIFSENGKDIILDTSSALLSQLVHKIKMLPFCHCLMQTTPQDHIVKQVCYLIASNNRIL 320
DAVRYLQTSVIKSPIVLLLAYAVCLPAAIICTKNETQLYSHCMRILKEYRPGDVMNILHESLTQHLNKCPSSTCAYTTRA 400
IVGTKANTTGLFFLPTQ
................................................................................ 80
.........................................................................aaaaaaa 160
aaaMaMaaaa...................................................................... 240
................................................................................ 320
................................................................................ 400
.................
//
NES-accession: NES-0059
Date: 14 Aug 2002
Protein: Importin beta-1 subunit (Karyopherin beta-1 subunit)
(Importin95) (Kap95p)
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: SWISS-PROT Q06142, IMB1_YEAST
Necessity: Aa 55-65 necessary in aa 1-77 fragment. L63A greatly impairs
export of full-length Kap95p.
Sufficiency: Aa 1-77 mediates export of GST.
Pathway: -
Location: -
Regulation: -
Comments: -
References: Iovine MK and Wente SR, 1997
J Cell Biol 137: 797-811. Medline: 9151683
Sequence: 861 aa
MSTAEFAQLLENSILSPDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKLEGRILAALTLKNELVSKDSVKTQQF 80
AQRWITQVSPEAKNQIKTNALTALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVDNTGAEQPENVKRASLLALG 160
YMCESADPQSQALVSSSNNILIAIVQGAQSTETSKAVRLAALNALADSLIFIKNNMEREGERNYLMQVVCEATQAEDIEV 240
QAAAFGCLCKIMSLYYTFMKPYMEQALYALTIATMKSPNDKVASMTVEFWSTICEEEIDIAYELAQFPQSPLQSYNFALS 320
SIKDVVPNLLNLLTRQNEDPEDDDWNVSMSAGACLQLFAQNCGNHILEPVLEFVEQNITADNWRNREAAVMAFGSIMDGP 400
DKVQRTYYVHQALPSILNLMNDQSLQVKETTAWCIGRIADSVAESIDPQQHLPGVVQACLIGLQDHPKVATNCSWTIINL 480
VEQLAEATPSPIYNFYPALVDGLIGAANRIDNEFNARASAFSALTTMVEYATDTVAETSASISTFVMDKLGQTMSVDENQ 560
LTLEDAQSLQELQSNILTVLAAVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVFYAISALAASLGKGFEKYLETF 640
SPYLLKALNQVDSPVSITAVGFIADISNSLEEDFRRYSDAMMNVLAQMISNPNARRELKPAVLSVFGDIASNIGADFIPY 720
LNDIMALCVAAQNTKPENGTLEALDYQIKVLEAVLDAYVGIVAGLHDKPEALFPYVGTIFQFIAQVAEDPQLYSEDATSR 800
AAVGLIGDIAAMFPDGSIKQFYGQDWVIDYIKRTRSGQLFSQATKDTARWAREQQKRQLSL
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaaaaaaaaaaaaaa.. 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
.............................................................
//
NES-accession: NES-0060
Date: 14 Aug 2002
Protein: BR1 protein
Organism: Squash leaf curl virus
DB_reference: SWISS-PROT P21935, VBR1_SLCV
Necessity: Aa 204-226 necessary in a deletional mutant context. Export of
full-length BR1 is abrogated by L216A/L217A/L220A.
Sufficiency: Aa 204-283 mediates export of GUS-NLS.
Pathway: -
Location: -
Regulation: -
Comments: Discrepancy of 27 in amino acid numbering between article and
retrieved sequence.
References: Ward BM and Lazarowitz SG, 1999,
Plant Cell 11: 1267-76. Medline: 10402428
Sequence: 283 aa
MRRIHILDNTCIIYTYIHCAVAIASSLMYSTSNRRGRSQTQRGSHVRRTGVKRSYGAARGDDRRRPNVVSKTQVEPRMTI 80
QRVQENQFGPEFVLSQNSALSTFVTYPSYVKTVPNRTRTYIKLKRVRFKGTLKIERGQGDTIMDGPSSNIEGVFSMVIVV 160
DRKPHVSQSGRLHTFDELFGARIHCHGNLSVVPALKDRYYIRHVTKRVVSLEKDTLLIDLHGTTQLSNKRYNCWASFSDL 240
ERDSCNGVYGNITKNALLVYYCWLSDAQSKASTYVSFELDYLG
................................................................................ 80
................................................................................ 160
...........................................aaaaaaaaaaaaMMaaMaaaaaaaaaaaaaaaaaaaa 240
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
//
NES-accession: NES-0061
Date: 14 Aug 2002
Protein: Fragile X mental retardation 1 protein (Protein FMR-1) (FMRP)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q06787, FMR1_HUMAN.
Necessity: Delta 425-41 results in nuclear localization.
Sufficiency: Aa 425-41 mediates export of BSA. Export is abrogated by
L430A/L432A/L435A.
Pathway: -
Location: Cytoplasmic, ribosome-associated.
Regulation: -
Comments: -
References: Eberhart DE, Malter HE, Feng Y, Warren ST, 1996,
Hum Mol Genet 5: 1083-91. Medline: 8842725
Sequence: 632 aa
MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWW 80
LAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVT 160
YDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGAN 240
IQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGKVIGKNGKLIQEIVDKSGVVRVRIEA 320
ENEKNVPQEEEIMPPNSLPSNNSRVGPNAPEEKKHLDIKENSTHFSQPNSTKVQRVLVASSVVAGESQKPELKAWQGMVP 400
FVFVGTKDSIANATVLLDYHLNYLKEVDQLRLERLQIDEQLRQIGASSRPPPNRTDKEKSYVTDDGQGMGRGSRPYRNRG 480
HGRRGPGYTSGTNSEASNASETESDHRDELSDWSLAPTEEERESFLRRGDGRRRGGGGRGQGGRGRGGGFKGNDDHSRTD 560
NRPRNPREAKGRTTDGSLQIRVDCNNERSVHTKTLQNTSSEGSRLRTGKDRNQKKEKPDSVDGQQPLVNGVP
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
........................aaaaaMaMaaMaaaaaa....................................... 480
................................................................................ 560
........................................................................
//
NES-accession: NES-0062
Date: 14 Aug 2002
Protein: Transcriptional regulator IE63 (VMW63) (ICP27)
Organism: Herpes simplex virus (type 1 / strain 17).
DB_reference: SWISS-PROT P10238, IE63_HSV11
Necessity: Delta aa 4-27 and L8R/L11R/L13R/L15R both abrogate export.
Sufficiency: Aa 5-17 mediates export of GFP.
Pathway: PKI-NES (NES-0001) can substitute, indicating common pathway.
Location: -
Regulation: -
Comments: -
References: Sandri-Goldin RM, 1998,
Genes Dev 12:868-79. Medline: 9512520
Sequence: 512 aa
MATDIDMLIDLGLDLSDSDLDEDPPEPAESRRDDLESDSSGECSSSDEDMEDPHGEDGPEPILDAARPAVRPSRPEDPGV 80
PSTQTPRPTERQGPNDPQPAPHSVWSRLGARRPSCSPEQHGGKVARLQPPPTKAQPARGGRRGRRRGRGRGGPGAADGLS 160
DPRRRAPRTNRNPGGPRPGAGWTDGPGAPHGEAWRGSEQPDPPGGQRTRGVRQAPPPLMTLAIAPPPADPRAPAPERKAP 240
AADTIDATTRLVLRSISERAAVDRISESFGRSAQVMHDPFGGQPFPAANSPWAPVLAGQGGPFDAETRRVSWETLVAHGP 320
SLYRTFAGNPRAASTAKAMRDCVLRQENFIEALASADETLAWCKMCIHHNLPLRPQDPIIGTTAAVLDNLATRLRPFLQC 400
YLKARGLCGLDELCSRRRLADIKDIASFVFVILARLANRVERGVAEIDYATLGVGVGEKMHFYLPGACMAGLIEILDTHR 480
QECSSRVCELTASHIVAPPYVHGKYFYCNSLF
....aaaMaaMaMaMaa............................................................... 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................
//
NES-accession: NES-0063
Date: 14 Aug 2002
Protein: Nonstructural protein NS1
Organism: Influenza A virus (strain A/Alaska/6/77).
DB_reference: SWISS-PROT P03494, VNS1_IAALA
Necessity: Aa 134-47 necessary in a deletional mutant context.
Sufficiency: Aa 138-47 mediates export of GST. Export abrogated by L144A and
L146A, whereas I145A had no effect.
Pathway: -
Location: Nuclear.
Regulation: NES masked by adjacent sequence, and NS1 (148-161). Exported
upon unmasking (during infection).
Comments: -
References: Li Y, Yamakita Y and Krug RM, 1998,
Proc Natl Acad Sci U S A 95: 4864-9. Medline: 9560194
Sequence: 237 aa
MDSNTVSSFQVDCFLWHVRKQVVDQELGDAPFLDRLRRDQRSLRGRGSTLGLDIEAATHVGKQIVEKILKEESDEALKMT 80
MASTPASRYITDMTIEELSRDWFMLMPKQKVEGPLCIRMDQAIMEKNIMLKANFSVIFDRLETLILLRAFTEEGAIVGEI 160
SPLLSFPGHTIEDVKNAIGVLIGGLEWNDNTVRVSKTLQRFAWGSSNENGGPPLTPKQKRKMARTARSKVRRDKMAD
................................................................................ 80
.........................................................aaaaaaMaMa............. 160
.............................................................................
//
NES-accession: NES-0064
Date: 14 Aug 2002
Protein: MAP kinase kinase 6 (MKK6)
Organism: Cyprinus carpio (Common carp)
DB_reference: TrEMBL Q9I959
Necessity: Both delta1-57, L46A/L50A and L53A/L55A abrogate export of MKK6.
Sufficiency: Aa 45-57 mediates export of ovalbumin.
Export abrogated by L46A/L50A/L53A/L55A.
Pathway: -
Location: Cytoplasmic.
Regulation: -
Comments: -
References: Hashimoto H, Fukuda M, Matsuo Y, Yokoyama Y, Nishida E,
Toyohara H and Sakaguchi M, 2000,
Eur J Biochem 2000 267: 4362-71. Medline: 10880959
Sequence: 404 aa
MMGHACGSSASDLLQMERTYLALLSRLPLSHLHSLSSHSLALSHLLSQDLEPLALSDRKCSSDRESRNSMGSRNKAGKKK 80
PPLKLPKEVFEKPQPAPTPPRDLDSKACVTIGDKNFVVKADDLEQIGELGRGAYGVVDKMRHVPSGVIMAVKRIRATVNT 160
QEQKRLLMDLDISMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYKQVHEKGMTIPEDILGKITVSIVKALEHLH 240
SNLSVIHRDVKPSNVLINMQGQVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPETNQKGYNVKSDIWSLGITMIEL 320
AILRFPYDSWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTSQCLRKNSKERPTYTELMQHPFFTIHDSKDTDVASFVKS 400
ILGD
............................................aMaaaMaaMaMaa....................... 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
....
//
NES-accession: NES-0065
Date: 14 Aug 2002
Protein: LIM protein SLIMMER
Organism: Homo sapiens (Human)
DB_reference: TrEMBL O95212
Necessity: -
Sufficiency: Aa 254-65 sufficient in a deletional mutant context.
Pathway: -
Location: Nuclear in myoblasts and cytoplasmic in myotubes
Regulation: -
Comments: -
References: Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM and
Mitchell CA, 1999,
J Biol Chem 274: 27083-91. Medline: 10480922
Sequence: 323 aa
MAEKFDCHYCRDPLQGKKYVQKDGHHCCLKCFDKFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETF 80
VAKDNKILCNKCTTREDSPKCKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQVIGTGSFFPKGEDFYCVTCHETKFAK 160
HCVKCNKAITSGGITYQDQPWHADCFVCVTCSKKLAGQRFTAVEDQYYCVDCYKNFVAKKCAGCKNPITGKRTVSRVSHP 240
VSKARKPPVCHGKRLPLTLFPSANLRGRHPGGERTCPSWVVVLYRKNRSLAAPRGPGLVKAPVWWPMKDNPGTTTASTAK 320
NAP
................................................................................ 80
................................................................................ 160
................................................................................ 240
..............aaaaaaaaaaa....................................................... 320
...
//
NES-accession: NES-0066
Date: 14 Aug 2002
Protein: Homeobox protein engrailed-2 (Gg-En-2)
Organism: Gallus gallus (Chicken)
DB_reference: SWISS-PROT Q05917, HME2_CHICK
Necessity: Delta 232-43 impaires export, but shuttling is still detected
in 20% of heterokaryons.
Sufficiency: Aa 229-48 mediates export of reporterprotein.
Pathway: -
Location: Nuclear
Regulation: -
Comments: -
References: Maizel A, Bensaude O, Prochiantz A, Joliot A, 1999,
Development 126: 3183-90. Medline: 10375508
Sequence: 288 aa
MEEGGRSPREEAAEPQESGGDAEPGGGRRALLLPPGDPPHPHPHPHRITNFFIDNILRPEFGRRKEAGGTAGEPRRPGAE 80
SRRSPAAAAPAPGAPVPGGGGGGGGGSPGRGEGGPAALALHGAAKKGGDPAALEAALKARGLSGAELSVSSDSDSSQAGS 160
NAGNQPMLWPAWVYCTRYSDRPSSPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELGLNE 240
SQIKIWFQNKRAKIKKATGSKNSLAVHLMAQGLYNHSTTAKDGKSDSE
................................................................................ 80
................................................................................ 160
....................................................................aaaaaaaaaaaa 240
aaaaaaaa........................................
//
NES-accession: NES-0067
Date: 14 Aug 2002
Protein: Nonsense-mediated mRNA decay protein 3 (Nmd3p)
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: SWISS-PROT P38861, NMD3_YEAST
Necessity: Delta 474-518 greatly impairs export, while delta 439-518
completely abrogates export.
Sufficiency: Aa 435-518 mediates export of NLS-GFP fusion.
Pathway: LMB-sensitive, Crm-dependent.
Location: Cytoplasmic.
Regulation: -
Comments: The assignment field for this sequenceis not accurate:
The experimental data propose 2 NESs, located from aa 445-56
and 492-503, respectively.
References: Ho JH, Kallstrom G, Johnson AW, 2000,
J Cell Biol 151: 1057-66. Medline: 11086007
Gadal O, Strauss D, Kessl J, Trumpower B, Tollervey D
and Hurt E, 2001,
Mol Cell Biol 21: 3405-15. Medline: 11313466
Sequence: 518 aa
MEFTPIDPHQHQNAATLLCCNCGTPIDGSTGLVMCYDCIKLTVDITQGIPREANISFCRNCERFLQPPGQWIRAELESRE 80
LLAICLRRLKGLTFVRLVDASFIWTEPHSRRIRIKLTVQGEAMTNTIIQQTFEVEYIVIAMQCPDCARSYTTNTWRATVQ 160
IRQKVPHKRTFLFLEQLILKHNAHVDTISISEAKDGLDFFYAQKNHAVKMIDFLNAVVPIKHKKSEELISQDTHTGASTY 240
KFSYSVEIVPICKDDLVVLPKKLAKSMGNISQFVLCSKISNTVQFMDPTTLQTADLSPSVYWRAPFNALADVTQLVEFIV 320
LDVDSTGISRGNRVLADITVARTSDLGVNDQVYYVRSHLGGICHAGDSVMGYFIANSNYNSDLFDGLNIDYVPDVVLVKK 400
LYQRKSKKSRHWKLKRMAKEHKDIDASLDYNSRAQKQEMERAEKDYELFLQELEEDAELRQSVNLYKNREANVPPEEHEM 480
DEDEDEDAPQINIDELLDELDEMTLEDGVENTPVESQQ
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
..................................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 480
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
//
NES-accession: NES-0068
Date: 14 Aug 2002
Protein: Forkhead box protein O3A (Forkhead in rhabdomyosarcoma-like 1)
(AF6q21 protein) (FKHRL1)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT O43524, FXO3_HUMAN
Necessity: Export abrogated by simultaneous mutation of both putative NESs
(369-78 and 386-96) by M376A/L378A/L382A/L390A/L391A/I394A/L396A.
Sufficiency: -
Pathway: LMB-sensitive.
Location: Nuclear.
Regulation: Exported upon phosphorylation by Akt and only when bound to
14-3-3 protein (the latter shown not to be providing the NES).
Comments: -
References: Brunet A, Kanai F, Stehn J, Xu J, Sarbassova D, Frangioni JV,
Dalal SN, DeCaprio JA, Greenberg ME, Yaffe MB, 2002
J Cell Biol 156: 817-28. Medline: 11864996
Sequence: 673 aa
MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIG 80
GGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNL 160
SYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDG 240
GKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTV 320
SGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQ 400
PSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLS 480
HSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLS 560
ESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDF 640
NFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
.......................................................M.M...M.......MM..M.M.... 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
.................................
//
NES-accession: NES-0069
Date: 14 Aug 2002
Protein: Histone deacetylase 5 (HD5) (Antigen NY-CO-9) (HDAC5)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q9UQL6, HDA5_HUMAN
Necessity: Delta 1081-1122, V1086 and L1092A all greatly impaires
or abrogates CaMK-mediated nuclear export.
Sufficiency: Aa 1081-22 NOT suffcient to mediate export of GFP, but suffcient
to mediate export of MITR - another 14-3-3 binding protein -
upon CaMK stimulation.
Pathway: LMB-sensitive.
Location: Nuclear.
Regulation: Export upon phosphorylation by CaMK.
Comments: Results indicative of additional sequence information being
necessary for export mediated by this NES.
References: McKinsey TA, Zhang CL and Olson EN, 2001,
Mol Cell Biol 21: 6312-21. Medline: 11509672
Sequence: 1122 aa
MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQEL 80
LALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRL 160
EQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKL 240
PLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPS 320
SPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAE 400
RQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATS 480
MRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQ 560
EVLLGEGALTMPREGSTESESTQEDLEEEDEEEDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQ 640
VYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQE 720
TGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSA 800
VRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQA 880
FYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVS 960
AGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPN 1040
INAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEP 1120
AL
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................................................ 880
................................................................................ 960
................................................................................ 1040
........................................aaaaaMaaaaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaa 1120
aa
//
NES-accession: NES-0070
Date: 14 Aug 2002
Protein: Histone deacetylase 4 (HD4) (HA6116) (HDAC4)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT P56524, HDA4_HUMAN
Necessity: V1056A greatly impaires, whereas L1062 abrogates the CaMK
mediated export (McKinsey et al. 2001).
Aa 1040-84 necessary in a deletional mutant context (Wang et al.
2001).
Sufficiency: Aa 1044-69 mediates export of GFP, but this export is abrogated
by either M1059A, L1062A, V1064A or V1066A (Wang et al. 2001).
Pathway: LMB-sensitivity.
Location: Nuclear.
Regulation: Exported upon phosphorylation by CaMK (McKinsey et al.
2001).
Comments: -
References: McKinsey TA, Zhang CL and Olson EN, 2001,
Mol Cell Biol 21: 6312-21. Medline: 11509672
Wang AH and Yang XJ, 2001,
Mol Cell Biol 21: 5992-6005. Medline: 11486037
Sequence: 1084 aa
MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQLQQELLALKQ 80
KQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEK 160
GKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFP 240
LRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDVTDSACSSAPGSGPSSPNNSSGSVSAENGIAPAV 320
PSIPAETSLAHRLVAREGSAAPLPLYTSPSLPNITLGLPATGPSAGTAGQQDTERLTLPALQQRLSLFPGTHLTPYLSTS 400
PLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHL 480
VIQQQHQQFLEKHKQQFQQQQLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEAHAQAGVQVKQ 560
EPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGGHRPLSRAQSSPASATFP 640
VSVQEPPTKPRFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHT 720
LLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPP 800
GHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEV 880
GTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQL 960
MGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGR 1040
SLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
................................................................................ 720
................................................................................ 800
................................................................................ 880
................................................................................ 960
................................................................................ 1040
...aaaaaaaaaaaaaaaMaaMaMaMaaa...............
//
NES-accession: NES-0071
Date: 14 Aug 2002
Protein: Transcription factor E2F4 (E2F-4)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q16254, E2F4_HUMAN
Necessity: I68A/L70A, delta 84-105 and L97S/I98R all lead to nuclear
accumulation, which is abolished by overexpression of Crm1.
Sufficiency: Since neither of the above-mentioned mutants are cytoplasmic,
it seems that they are not 2 independently sufficient NESs.
Pathway: LMB-sensitive, Crm-dependent.
Location: Cytoplasmic.
Regulation: -
Comments: Atypical long NES.
References: Gaubatz S, Lees JA, Lindeman GJ, Livingston DM, 2001,
Mol Cell Biol 21: 1384-92. Medline: 11158323
Sequence: 413 aa
MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQ 80
WKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAP 160
SGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEAS 240
RPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSN 320
SNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESE 400
GVCDLFDVPVLNL
...................................................................M.M.......... 80
................MM.............................................................. 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
.............
//
NES-accession: NES-0072
Date: 14 Aug 2002
Protein: DNA damage checkpoint protein rad24
Organism: Schizosaccharomyces pombe (Fission yeast)
DB_reference: SWISS-PROT P42656, RA24_SCHPO
Necessity: I222A/L226A greatly impairs export.
Sufficiency: -
Pathway: Crm-dependency.
Location: Cytoplasmic.
Regulation: -
Comments: -
References: Lopez-Girona A, Furnari B, Mondesert O and Russell P, 1999,
Nature 397: 172-5. Medline: 9923681
Sequence: 270 aa
MSTTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQ 80
VELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEI 160
ATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAEYS 240
AAAAGGNTEGAQENAPSNAPEGEAEPKADA
................................................................................ 80
................................................................................ 160
.............................................................M...M.............. 240
..............................
//
NES-accession: NES-0073
Date: 14 Aug 2002
Protein: Serine/threonine protein kinase 4 (STE20-like kinase MST1)
(MST-1) (Mammalian STE20-like protein kinase 1)
(Serine/threonine protein kinase Krs-2)
Organism: Homo sapiens (Human)
DB_reference: SWISS-PROT Q13043, STK4_HUMAN
Necessity: Only simultaneous mutation of both NESs abrogates export
(M368A/I370A/W439A/V441A or M368A/I370A/V441A/L444A).
Sufficiency: The fact that MST1 is still excluded from the nucleus when only
1 NES is mutated at the time, is indicative of each NES being
sufficient.
Pathway: LMB-sensitive.
Location: Cytoplasmic.
Regulation: Dimerization facilitates cytoplasmic retention, whereas
phosphorylation stimulates import.
Comments: -
References: Lee KK and Yonehara S, 2002,
J Biol Chem 277: 12351-8. Medline: 11805089
Sequence: 487 aa
METVQLRNPPRRQLKKLDEDSLTKQPEEVFDVLEKLGEGSYGSVYKAIHKETGQIVAIKQVPVESDLQEIIKEISIMQQC 80
DSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYLHFMRKIHRDIKAGNILLNTE 160
GHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNP 240
PPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPFVRSAKGVSILRDLINEAMDVKLKRQESQQREVDQDDEENS 320
EEDEMDSGTMVRAVGDEMGTVRVASTMTDGANTMIEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFE 400
QKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAK 480
KRRQQNF
................................................................................ 80
................................................................................ 160
................................................................................ 240
................................................................................ 320
...............................................M.M.............................. 400
......................................M.M..M.................................... 480
.......
//
NES-accession: NES-0074
Date: 14 Aug 2002
Protein: Iron-regulated transcriptional repressor AFT1
Organism: Saccharomyces cerevisiae (Baker's yeast)
DB_reference: SWISS-PROT P22149, AFT1_YEAST. NLSdb NLS:270
Necessity: L99A and L102A constitutively nuclear even in iron-replete cells.
Sufficiency: -
Pathway: -
Location: Nuclear in iron-deplete cells, and cytoplasmic in iron-replete
cells.
Regulation: -
Comments: -
References: Yamaguchi-Iwai Y, Ueta R, Fukunaka A, Sasaki R, 2002,
J Biol Chem 277: 18914-8. Medline: 11877447
Sequence: 690 aa
MEGFNPADIEHASPINSSDSHSSSFVYALPKSASEYVVNHNEGRASASGNPAAVPSPIMTLNLKSTHSLNIDQHVHTSTS 80
PTETIGHIHHVEKLNQNNLIHLDPVPNFEDKSDIKPWLQKIFYPQGIELVIERSDAFKVVFKCKAAKRGRNARRKRKDKP 160
KGQDHEDEKSKINDDELEYASPSNATVTNGPQTSPDQTSSIKPKKKRCVSRFNNCPFRVRATYSLKRKRWSIVVMDNNHS 240
HQLKFNPDSEEYKKFKEKLRKDNDVDAIKKFDELEYRTLANLPIPTATIPCDCGLTNEIQSFNVVLPTNSNVTSSASSST 320
VSSISLDSSNASKRPCLPSVNNTGSINTNNVRKPKSQCKNKDTLLKRTTMQNFLTTKSRLRKTGTPTSSQHSSTAFSGYI 400
DDPFNLNEILPLPASDFKLNTVTNLNEIDFTNIFTKSPHPHSGSTHPRQVFDQLDDCSSILFSPLTTNTNNEFEGESDDF 480
VHSPYLNSEADFSQILSSAPPVHHDPNETHQENQDIIDRFANSSQEHNEYILQYLTHSDAANHNNIGVPNNNSHSLNTQH 560
NVSDLGNSLLRQEALVGSSSTKIFDELKFVQNGPHGSQHPIDFQHVDHRHLSSNEPQVRSHQYGPQQQPPQQLQYHQNQP 640
HDGHNHEQHQTVQKDMQTHESLEIMGNTLLEEFKDIKMVNGELKYVKPED
................................................................................ 80
..................M..M.......................................................... 160
................................................................................ 240
................................................................................ 320
................................................................................ 400
................................................................................ 480
................................................................................ 560
................................................................................ 640
..................................................
//
NES-accession: NES-0075
Date: 14 Aug 2002
Protein: Transcriptional regulator IE63 homolog (Diffuse early antigen)
(SM) (BMLF1)
Organism: Epstein-barr virus (strain B95-8) (Human herpesvirus 4)
DB_reference: SWISS-PROT Q04360, IE63_EBV
Necessity: Delta 227-36 and V184A/T185A/L186A/L193A/T194A/L195A both
abrogate shuttling in heterokaryon assay, whereas
L193A/T194A/L195A does not.
Sufficiency: Aa 186-95 and aa 177-86 are each independently sufficient to
mediate export of a GFP-NLS-PK fusion protein.
Pathway: LMB sensitive.
Location: Nuclear.
Regulation: -
Comments: 2 overlapping NESs (designated "A/M" and "a/M", respectively).
Discrepancy of 41 in amino acid numbering between article and
retrieved sequence.
References: Chen L, Liao G, Fujimuro M, Semmes OJ and Hayward SD, 2001,
Virology 288: 119-28. Medline: 11543664
Sequence: 438 aa
MEGSEEHSTDGEISSSEEEDEDPTPAHAIPARPSSVVITPTSASFVIPRKKWDLQDKTVTLHRSPLCRDEDEKEETGNSS 80
YTRGHKRRRGEVHGCTDESYGKRRHLPPGARAPRAPRAPRVPRAPRSPRAPRSNRATRGPRSESRGAGRSTRKQARQERS 160
QRPLPNKPWFDMSLVKPVSKITFVTLPSPLASLTLEPIQDPFLQSMLAVAAHPEIGAWQKVQPRHELRRSYKTLREFFTK 240
STNKDTWLDARMQAIQNAGLCTLVAMLEETIFWLQEITYHGDLPLAPAEDILLACAMSLSKVILTKLKELAPCFLPNTRD 320
YNFVKQLFYITCATARQNKVVETLSSSYVKQPLCLLAAYAAVAPAYINANCRRRHDEVEFLGHYIKNYNPGTLSSLLTEA 400
VETHTRDCRSASCSRLVRAILSPGTGSLGLFFVPGLNQ
................................................................................ 80
................................................................................ 160
................AAAAAAAMMMaaaaaaMMM............................................. 240
................................................................................ 320
................................................................................ 400
......................................
//