NES-accession:	NES-0001
Date:		14 Aug 2002
Protein:	cAMP-dependent Protein Kinase Inhibitor, alpha form (PKI-alpha).
Organism:	Homo sapiens (Human) / Oryctolagus cuniculus (Rabbit).
DB_reference:	SWISS-PROT P04541, IPKA_HUMAN.
Necessity:	Export abrogated by the following mutations: L37A/L39A/L41A,
		 L37A/L41A, L41A/L44A, L44A/I46A, L44A and I46A.
Sufficiency:	Aa 37-46 mediates export of GST.
Pathway:	LMB-sensitive.
Location:	- 
Regulation:	-
Comments:	-
References:	Wen W, Meinkoth JL, Tsien RY and Taylor SS, 1995,
                  Cell 82: 463-73. Medline: 7634336
		Henderson and Eleftheriou, 2000,
		  Exp. Cell Res. 256: 213-224. Medline: 10739668
Sequence:	75 aa
TDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEEDAQRSSTEQSGEAQGEAAKSES
....................................MaMaMaaMaM.............................
//
NES-accession:	NES-0002
Date:		14 Aug 2002
Protein:	Cellular tumor antigen p53 (Tumor suppressor p53)
		(Phosphoprotein p53) (Antigen NY-CO-13)
Organism:	Homo sapiens (Human).
DB_reference:	SWISS-PROT P04637, P53_HUMAN. NLSdb NLS:76, NLS:127
Necessity:	L348A/L350A does not shuttle in heterokaryon assay (Stommel et
		al. 1999)
Sufficiency:	Aa 339-352 mediates export of BSA (stommel et al. 1999). 
		Zhang and Xiong 2001 further shows aa 11-27 to mediate export
		of GFP, which is abrogated by the L14Q/F19S or L22Q/W23S
		mutations. This is argued though, by Inoue et al. 2002, who
		shows that p53 aa 7-27 cannot function as an autonomous NES. 
Pathway:	LMB-sensitive 
Location:	Nucleus
Regulation:	Regulation by masking the NES upon tetramerization (Stommel
		et al. 1999). Phosphorylation of Ser15, abrogates export (Zhang 
		and Xiong 2001)
Comments:	There is controversy regarding the N-terminal NES (Zhang and
		Xiong 2001, Inoue et al. 2002), why it has not been included 
		in the assignment field.
References:	Stommel JM, Marchenko ND, Jimenez GS, Moll UM, Hope TJ and 
		Wahl GM, 1999
		  EMBO J 18: 1660-72. Medline: 10075936		
		Zhang Y and Xiong Y, 2001,
                  Science 292: 1910-5. Medline: 11397945
		Inoue T, Stuart J, Leno R, Maki CG, 2002,
		  J. Biol. Chem. 277: 15053-60. Medline: 11847229
Sequence:	393 aa
MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAP        80
TPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAM       160
AIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS       240
SCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKK       320
KPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
................................................................................        80
................................................................................       160
................................................................................       240
................................................................................       320
..................aaaaaaaaaMaMaa.........................................
//
NES-accession:	NES-0003
Date:		14 Aug 2002
Protein:	Ubiquitin-protein ligase E3 Mdm2 (p53-binding protein Mdm2)
		(Oncoprotein Mdm2) (Double minute 2 protein) (Hdm2)
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT Q00987, MDM2_HUMAN
Necessity:	Mutation of L199A/I202A greatly impaires nuclear shuttling in a
		heterokaryon-assay
Sufficiency:	Aa 188-202 mediates export of GFP 
		Aa 191-205 mediates export of GST 
Pathway:	Rev-like pathway 
Location:	Nucleus
Regulation:	-
Comments:	Discrepancy on Aa numbering: There is a difference of 6 in the
		numbering of amino acids between this sequence and these articles
References:	Roth J, Dobbelstein M, Freedman DA, Shenk T, Levine AJ, 1998
		EMBO J 17: 554-64. Medline: 9430646	
		Zhang Y and Xiong Y, 2001
		Science 292: 1910-5. Medline: 11397945
Sequence:	491 aa 
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSND        80
LLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSS       160
RRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDS       240
VSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCN       320
RCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTS       400
SSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQ       480
PIQMIVLTYFP
................................................................................        80
................................................................................       160
...........................aaaaaaaaaaaMaaM......................................       240
................................................................................       320
................................................................................       400
................................................................................       480
...........
//
NES-accession:	NES-0004
Date:		14 Aug 2002
Protein:	Xsmad4a
Organism:	Xenopus laevis (African clawed frog).
DB_reference:	TrEMBL Q9W639
Necessity:	Necessary signal within the region of aa 134-320 
		(Deletional mutant context)
Sufficiency:	Aa 137-46 mediates export of ovalbumin
Pathway:	LMB-sensitive
Location:	Cytoplasmic
Regulation:	-
Comments:	-
References:	Watanabe M, Masuyama N, Fukuda M and Nishida E, 2000	
		EMBO Rep 1: 176-82. Medline: 11265759
Sequence:	549 aa  
MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQ        80
VAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSSAPSGLLVKDEYG       160
HDYAEGQQSISAGDGLSIQTIQHPPSSRSSTETYSNPALLVPSEPSTPNPTSFSSIPVPSTSQPASLLAATHNDGLLSIA       240
PVPPQGTQNGFSAQPATYHHNSTTTWTGSRTAAYTPNMSHHPNGHLQHHPPMPHPGHYWPVHNELAFQPPISNHPAPDYW       320
CSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLS       400
DHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAIS       480
LSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD
................................................................................        80
........................................................aaaaaaaaaa..............       160
................................................................................       240
................................................................................       320
................................................................................       400
................................................................................       480
.....................................................................
//
NES-accession:	NES-0005
Date:		14 Aug 2002
Protein:	p120 catenin isoform 3AB
Organism:	Homo sapiens (Human)
DB_reference:	GenBank AF062338.1
Necessity: 	Exon B necessary for export
Sufficiency:	Aa 755-71 mediates export of ovalbumin.
Pathway:	LMB-sensitive
Location:	Isoforms containing exon B are exclusively cytoplasmic.
		Isoforms lacking exon B are equally distributed between nucleus
		and cytoplam
Regulation:	Regulation by alternative splicing. NES located in exon B. 
Comments:	32 different human isoforms
References:	van Hengel J, Vanhoenacker P, Staes K and van Roy F, 1999
		Proc Natl Acad Sci U S A 96: 7980-5. Medline: 10393933
Sequence:	861 aa   
MQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRD        80
FRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQP       160
QVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQ       240
HERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPK       320
KEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEV       400
IIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVC       480
LLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRI       560
YISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELI       640
GKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQ       720
TIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTP       800
NERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI
................................................................................        80
................................................................................       160
................................................................................       240
................................................................................       320
................................................................................       400
................................................................................       480
................................................................................       560
................................................................................       640
................................................................................       720
................................................................................       800
..................................aaaaaaaaaMaMaaaaa..........
//
NES-accession:	NES-0006
Date:		14 Aug 2002
Protein:	MAP KINASE-ACTIVATED PROTEIN KINASE 2 
		(MAPK-ACTIVATED PROTEIN KINASE 2) (MAPKAP KINASE 2) (MAPKAPK-2)
Organism:	Mus musculus (Mouse)
DB_reference:	SWISS-PROT P49138, MKK2_MOUSE
Necessity:	A L345A mutation of the otherwise exclusively cytoplasmic T319E 
		mutant, results in nuclear localization
Sufficiency:	Aa 330-353 is sufficient to mediate export of GFP, whereas
		neither aa 341-351 nor aa 337-353 are	
Pathway:	LMB-sensitive
Location:	Predominantly nuclear
Regulation:	Phosphorylation of  T319, but not T207, unmasks NES and allows
			export
Comments:	Atypical NES with weak similarity to EAIV and FIV Rev NESs.
		Discrepancy of 2 in amino acid numbering between article and 
		retrieved sequence 
References:	Engel K, Kotlyarov A and Gaestel M, 1998	
		EMBO J 17: 3363-71. Medline: 9628873
Sequence:	385 aa   
IRGSPGQTPPAPFPSPPPPAPAQPPPPFPQFHVKSGLQIRKNAITDDYKVTSQVLGLGINGKVLRIFDKRTQQKFALKML      80
QDCPKARREVELTWRASQCPHIVHIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQ     160
YLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYI     240
LLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTP     320
LHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARAVEDAALAH
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
.........aaaaaaaaaaaaaaaMaaaaaaaa................................
//
NES-accession:	NES-0007
Date:		14 Aug 2002
Protein:	PROTO-ONCOGENE TYROSINE-PROTEIN KINASE ABL1 (P150) (C-ABL)
Organism:	Mus musculus (Mouse)
DB_reference:	SWISS-PROT P00520, ABL1_MOUSE
Necessity:	L1090A mutation results in exclusively nuclear localization of 
		both full-length protein and aa 998-1079.
Sufficiency:	Aa 1078-96 mediates export of GST
Pathway:	LMB-sensitive
Location:	Predominantly nuclear
Regulation:	-
Comments:	Discrepancy of 26 in amino acid numbering between article and
		retrieved sequence.There is no sequence in the databases with 
		the same aa numbering as the in the article
References:	Taagepera S, McDonald D, Loeb JE, Whitaker LL, McElroy AK,
		Wang JY and Hope TJ, 1998
		Proc Natl Acad Sci U S A 95: 7457-62. Medline 9636171
Sequence:	1123 aa  
MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTL      80
SITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQ     160
RSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERT     240
DITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTY     320
GNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAK     400
FPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNP     480
SDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESD     560
ALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRE     640
LCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASC     720
MPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPLPGWLKKNEEAAEEGFKDT     800
ESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPG     880
RDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQS     960
TAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNS    1040
EQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDI    1120
VRR
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
................................................................................     800
................................................................................     880
................................................................................     960
................................................................................    1040
.....................................aaaaaaaaaaaaMaaaaaa........................    1120
...
//
NES-accession:	NES-0008
Date:		14 Aug 2002
Protein:	G2/MITOTIC-SPECIFIC CYCLIN B1
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT P14635, CGB1_HUMAN
Necessity:	V149A/L151A/V153A mutation abrogates export of full-length 
		protein
Sufficiency:	Aa 141-52 mediates export of ovalbumin
Pathway:	LMB-sensitive
Location:	Cytoplasmic during S and G(2)-phases, but nuclear during
		prophase
Regulation:	-
Comments:	-
References:	Toyoshima F, Moriguchi T, Wada A, Fukuda M and Nishida E, 1998
		EMBO J 17: 2728-35. Medline: 9582266
Sequence:	433 aa   
MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLP      80
KPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAED     160
GADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVP     240
KKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQH     320
TLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTV     400
KNKYATSKHAKISTLPQLNSALVQDLAKAVAKV
................................................................................      80
............................................................aaaaaaaaMaMaM.......     160
................................................................................     240
................................................................................     320
................................................................................     400
.................................
//
NES-accession:	NES-0009
Date:		14 Aug 2002
Protein:	Actin
Organism:	Saccharomyces cerevisiae (Baker's yeast)
DB_reference:	SWISS-PROT P02579, ACT_YEAST
Necessity:	Mutation of both NESs (I178A/L180A/V219A/L221A), reults in 
		exclusively nuclear localization. Mutation of one NES at the
		time results in inhibition of export
Sufficiency:	Both NESs individually sufficient to mediate export of ovalbumin. 
		Shown with peptides of mammalian actin alfa corresponding to 
		Sc actin aa 170-81 and aa 211-22 respectively
Pathway:	LMB-sensitive
Location:	Cytoplasmic
Regulation:	-
Comments:	2 NESs in this sequence.
References:	Wada A, Fukuda M, Mishima M and Nishida E, 1998	
		EMBO J 17: 1635-41. Medline: 9501085
Sequence:	375 aa  
MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWD      80
DMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVT     160
HVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY     240
ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITAL     320
APSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF
................................................................................      80
................................................................................     160
.........aaaaaaaaMaMa.............................aaaaaaaaMaMa..................     240
................................................................................     320
.......................................................
//
NES-accession:	NES-0010
Date:		14 Aug 2002
Protein:	HIV-1 Rev protein
Organism:	Human immunodeficiency virus type 1 (CDC-451 isolate) (HIV-1)
DB_reference:	SWISS-PROT P05865, REV_HV1C4. NLSdb NLS:166
Necessity:	-
Sufficiency:	Aa 75-83 mediates export of GST and BSA. Export abrogated by the
		L78D/E79L and L83A mutations.
Pathway:	LMB-sensitive (Henderson and Eleftheriou 2000).
Location:	-
Regulation:	-
Comments:	-
References:	Fischer U, Huber J, Boelens WC, Mattaj IW and Luhrmann R, 1995
		Cell 82: 475-83. Medline: 7543368
		Henderson BR and Eleftheriou A, 2000
		Exp Cell Res 2000 256: 213-24, Medline: 10739668
Sequence:	116 aa 		   
MAGRSGDSDEELIKTVRIIKHLYQSNPPPKPEGTRQARRNRRRRWRERQRRIHSISAWLLSTCLGRSAEPVPLQLPPLER        80
LTLDCSEDCGTSGTQGVGSPQILVESPAVLESGTKE
..........................................................................aaaMMa        80
aaM.................................
//
NES-accession:	NES-0011
Date:		14 Aug 2002
Protein:	Rex protein
Organism:	Human T-lymphotropic virus 1
DB_reference: 	TrEMBL Q82233 
Necessity:	-
Sufficiency:	Aa 81-94 mediates export of GST. Export abrogated by
		L90A/L92A.
Pathway:	-
Location:	-
Regulation:	-
Comments:	-
References:	Bogerd HP, Fridell RA, Benson RE, Hua J and Cullen BR, 1996
		Mol Cell Biol 16: 4207-14. Medline: 8754820
Sequence:	189 aa    
MPKTRRRPRRSQRKRPPTPWPTSQGLDRVFFSDTQSTCLETVYKATGAPSLGDYVRPAYIVTPYWPPVQSIRSPGTPSMD        80
ALSAQLYSSLSLDSPPSPPREPLRPSRSLPRQSLIQPPTFHPPSSRPCANTPPSEMDTWNPPLGSTSQPCLFQTPDSGPK       160
TCTPSGEAPLSACTSTSFPPPSPGPSCPT
...............................................................................a        80
aLaaaLaaaMaMaa..................................................................       160
.............................
//
NES-accession:	NES-0012
Date:		14 Aug 2002
Protein:	Dual specificity mitogen-activated protein kinase kinase 1 
		(MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) 
		(MAPK/ERK kinase 1) (MEK1)
Organism:	Xenopus laevis (African clawed frog)	
DB_reference:	SWISS-PROT Q05116, MPK1_XENLA
Necessity:	Delta 31-50, L32A/L36A and L39A/L41A all lead to whole cell
		distribution
Sufficiency: 	Aa 32-44 mediates export of ovalbumin, which is abrogated
		by L32A/L36A/L39A/L41A
Pathway:	LMB-sensitive
Location:	Cytoplasmic
Regulation:	-	
Comments:	Discrepancy of 1 in amino acid numbering between article and
		retrieved sequence
References:	Fukuda M, Gotoh I, Gotoh Y and Nishida E, 1996	
		J Biol Chem 271: 20024-8. Medline: 8702720
		Fukuda M, Asano S, Nakamura T, Adachi M, Yoshida M, 
		Yanagida M and Nishida E, 1997
		Nature 390: 308-11. Medline: 9384386
Sequence:	394 aa   
PKKKPTPIQLNPNPEGTAVNGTPTAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKVSELGAGNGGV      80
VFKVSHKPTSLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGKI     160
PEKILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYS     240
VQSDIWSMGLSLVEMAIGRYPIPPPDAKELELIFGCSVERDPASSELAPRPRPPGRPISSYGPDSRPPMAIFELLDYIVN     320
EPPPKLPSGVFGAEFQDFVNKCLVKNPAERADLKQLMVHSFIKQSELEEVDFAGWLCSTMGLKQPSTPTHAAGV
...............................MaaaMaaMaMaaa....................................      80
................................................................................     160
................................................................................     240
................................................................................     320
..........................................................................
//
NES-accession:	NES-0013
Date:		14 Aug 2002
Protein:	EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15
		(PROTEIN EPS15) (AF-1P PROTEIN)
Organism:	Homo sapiens (Human)		
DB_reference:	SWISS-PROT P42566, EP15_HUMAN
Necessity:	Aa 766-896 necessary in full-length context. Aa 849-896
		necessary in a deletional mutant context. I893A, L882A/L884A and
		I886A/L888A leads to nucelar accumulation. Aa 890-96 indirectly
		necessary, since aa 766-896 is sufficient to mediate export of
		GFP, whereas aa 766-890 is not
Sufficiency: 	Aa 766-896 is mediates export of GFP, whereas aa 766-890 does not.
Pathway:	LMB-sensitive
Location:	Cytoplasmic	
Regulation:	-
Comments:	-
References:	Poupon V, Polo S, Vecchi M, Martin G, Dautry-Varsat A,
		Cerf-Bensussan N, Di Fiore PP and Benmerah A, 2002	
		J Biol Chem 277: 8941-8. Medline: 11777906
Sequence:	896 aa   
MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNKQEFFVALRLV      80
ACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKL     160
PVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMD     240
GFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNI     320
IGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDE     400
LDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQ     480
HLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPA     560
RSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKID     640
PFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESF     720
GGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPF     800
KLNDPFQPFPGNDSPKEKDPEMFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQE     880
DLELAIALSKSEISEA
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
.............................................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa     800
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa     880
aMaMaMaMaaaaMaaa
//
NES-accession:	NES-0014
Date:		14 Aug 2002
Protein:	Breast cancer type 1 susceptibility protein (BRCA1)
Organism:	Homo sapiens (Human)	
DB_reference:	SWISS-PROT P38398, BRC1_HUMAN. NLSdb NLS:49
Necessity:	L86A/I90A abrogates export of full-length protein
Sufficiency: 	Aa 81-99 mediates export of GFP
Pathway:	LMB-sensitive and Crm-dependent	
Location:	Whole cell distribution in most cells, but also exclusively
		either nuclear or cytoplasmic in some cells	
Regulation:	-
Comments:	-
References:	Rodriguez JA, Henderson BR, 2000
		J Biol Chem 275: 38589-96. Medline: 10991937
Sequence:	1863 aa 	
MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFS      80
QLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLG     160
TVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQ     240
PSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNR     320
WAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHD     400
GESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTEN     480
LIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGD     560
SIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQ     640
IDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKE     720
FVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNK     800
CVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEE     880
ECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRG     960
NETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEAS    1040
SSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTV    1120
NTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQ    1200
GYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKAS    1280
QEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQS    1360
MDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALE    1440
DLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNR    1520
NYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSAL    1600
KVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLI    1680
TEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKI    1760
FRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLD    1840
SVALYQCQELDTYLIPQIPHSHY
................................................................................      80
aaaaaMaaaMaaaaaaaaa.............................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
................................................................................     800
................................................................................     880
................................................................................     960
................................................................................    1040
................................................................................    1120
................................................................................    1200
................................................................................    1280
................................................................................    1360
................................................................................    1440
................................................................................    1520
................................................................................    1600
................................................................................    1680
................................................................................    1760
................................................................................    1840
.......................
//
NES-accession:	NES-0015
Date:		14 Aug 2002
Protein:	Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (RelA)
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT Q04206, TF65_HUMAN. NLSdb NLS:17
Necessity:	Delta 440-43 abrogates export of full-length protein	 		
Sufficiency: 	Aa 436-45 is mediates export of a p50-GFP fusion construct	 
Pathway:	LMB-sensitive
Location:	Cytoplasmic when not complexed with p50 subunit of NF-kappa-B					
Regulation:	-
Comments:	-
References:	Harhaj EW and Sun SC, 1999
		Mol Cell Biol 19: 7088-95. Medline: 10490645
Sequence:	551 aa   
MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKD      80
PPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLC     160
FQVTVRDPSGRPLRLPPVLPHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFS     240
QADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSG     320
PTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMV     400
SALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQ     480
GIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
...................................aaaaMMMMaa...................................     480
.......................................................................
//
NES-accession:	NES-0016
Date:		14 Aug 2002
Protein:	Heat shock factor protein HSF30 (Heat shock transcription factor
		30) (HSTF 30) (Heat stress transcription factor) (HsfA2)
Organism:	Lycopersicon peruvianum (Peruvian tomato)
DB_reference:	SWISS-PROT P41152, HSF3_LYCPE
Necessity:	Deletion of the most C-terminal 8 residues or mutation of
		L344A/L348A/L351A abrogates export
Sufficiency: 	C-terminal 23 aa mediates export of the otherwise completely
		nuclear HsfB1. 
Pathway:	LMB-sensitive
Location:	Cytosolic
Regulation:	Becomes nuclear upon coexpression of HsfA1
Comments:	-
References:	Heerklotz D, Doring P, Bonzelius F, Winkelhaus S 
		and Nover L, 2001
		Mol Cell Biol 21: 1759-68. Medline: 11238913
Sequence:	351 aa   
MEDVMKVKVEEDGIPTAVLPMEGLHDVGPPPFLSKTYEMVEDSSTDQVISWSTTRNSFIVWDSHKFSTTLLPRFFKHSNF      80
SSFIRQLNTYGFRKVDPDRWEFANEGFLGGQKHLLKTIKRRRNVGQSMNQQGSGACIEIGYYGMEEELERLKRDKNVLMT     160
EIVKLRQQQQSTRNQIIAMGEKIETQERKQVQMMSFLAKIFSNPTFLQQYLDKQVHRKDKQRIEVGQKRRLTMTPSVTGS     240
DQPMNYSSSLQESEAELASIEMLFSAAMDNESSSNVRPDSVVTANGTDMEPVADDIWEELLSEDLISGDRAAEEVVVVEQ     320
PEFDVEVEDLVVKTPEWGEELQDLVDQLGFL
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
.......aaaaaaaaaaaaaaaaMaaaMaaM
//
NES-accession:	NES-0017
Date:		14 Aug 2002
Protein:	AP-1-like transcription factor (Pap1)
Organism:	Schizosaccharomyces pombe (Fission yeast)
DB_reference:	SWISS-PROT Q01663, AP1_SCHPO
Necessity:	Delta 488-544 abrogates export
Sufficiency: 	Aa 515-533 mediates export of GST-GFP fusion. Export 
		abrogated by L522A, L526A, F517A/I519A and C523S/C532S and 
		greatly impaired by I519A and C532S
Pathway:	Crm-dependent, LMB-sensitive
Location:	Cytoplasmic
Regulation:	Regulated by oxidation of cysteine in the NES region
Comments:	-
References:	Kudo N, Taoka H, Toda T, Yoshida M and Horinouchi S, 1999	
		J Biol Chem 274: 15151-8. Medline: 10329722
Sequence:	552 aa   
MSGQTETLSSTSNIPIAKAEPEQSADFSASHKKRGPVSDRSSRRTSSEEVDLMPNVDDEVDGDVKPKKIGRKNSDQEPSS      80
KRKAQNRAAQRAFRKRKEDHLKALETQVVTLKELHSSTTLENDQLRQKVRQLEEELRILKDGSFTFEMSLPHRNPSLSSL     160
PTTGFSSNFAHMKDGISPQSNLHLSPNSIEKPNMHQNVLHNDRSADNLNHRYQVPPTLVDSNSAQGTLSPETPSSSDSPS     240
NLYLNYPKRKSITHLHHDCSALSNGENGEDVADGKQFCQKLSTACGSIACSMLTKTTPHRASVDILSNLHESTVSPPMAD     320
ESVQRSSEVSKSIPNVELSLNVNQQFVSPFGGTDSFPLPTDTGLDSLFEPDSAIENSHLKNVVMEPELFQAWREPAESLD     400
KEFFNDEGEIDDVFHNYFHNSNENGDLITNSLHGLDFLENANESFPEQMYPFIKHNKDYISNHPDEVPPDGLPQKGKHDT     480
SSQMPSENEIVPAKERAYLSCPKVWSKIINHPRFESFDIDDLCSKLKNKAKCSSSGVLLDERDVEAALNQFN
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
..................................aaMaMaaMMaaMaaaaaMa...................
//
NES-accession:	NES-0018
Date:		14 Aug 2002
Protein:	Signal transducer and activator of transcription 1-alpha/beta 
		(Transcription factor ISGF-3 components p91/p84)
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT P42224, STA1_HUMAN
Necessity:	L199A greatly impairs export
Sufficiency: 	Aa 197-205 sufficient to mediate export of GFP
Pathway:	LMB-sensitivity, Crm-interaction
Location:	-	
Regulation:	-
Comments:	-
References:	Mowen K and David M, 2000
		Mol Cell Biol 20: 7273-81. Medline: 10982844
Sequence:	750 aa   
MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQ      80
HNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEI     160
KSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWK     240
RRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVE     320
RQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSL     400
AAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAE     480
PRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIES     560
ILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELS     640
AVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTT     720
DNLLPMSPEEFDEVSRIVGSVEFDSMMNTV
................................................................................      80
................................................................................     160
....................................aaMaaaaaa...................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
..............................
//
NES-accession:	NES-0019
Date:		14 Aug 2002
Protein:	Metal-regulatory transcription factor (MTF1)
Organism:	Homo sapiens (Human)
DB_reference:	TrEMBL Q14872
Necessity:	L338A/L341A/L343A/L344A abrogates export of full-length MTF1
Sufficiency: 	Aa 336-44 mediates nuclear exclusion of tandem eGFP
Pathway:	LMB-sensitive
Location:	-
Regulation:	-
Comments:	-
References:	Saydam N, Georgiev O, Nakano MY, Greber UF and Schaffner W, 2001
		J Biol Chem 276: 25487-95. Medline: 11306562
Sequence:	753 aa    
MGEHSPDNNIIYFEAEEDELTPDDKMLRFVDKNGLVPSSSGTVYDRTTVLIEQDPGTLEDEDDDGQCGEHLPFLVGGEEG        80
FHLIDHEAMSQGYVQHIISPDQIHLTINPGSTPMPRNIEGATLTLQSECPETKRKEVKRYQCTFEGCPRTYSTAGNLRTH       160
QKTHRGEYTFVCNQEGCGKAFLTSHSLRIHVRVHTKEKPFECDVQGCEKAFNTLYRLKAHQRLHTGKTFNCESEGCSKYF       240
TTLSDLRKHIRTHTGEKPFRCDHDGCGKAFAASHHLKTHVRTHTGERPFFCPSNGCEKTFSTQYSLKSHMKGHDNKGHSY       320
NALPQHNGSEDTNHSLCLSDLSLLSTDSELRENSSTTQGQDLSTISPAIIFESMFQNSDDTAIQEDPQQTASLTESFNGD       400
AESVSDVPPSTGNSASLSLPLVLQPGLSEPPQPLLPASAPSAPPPAPSLGPGSQQAAFGNPPALLQPPEVPVPHSTQFAA       480
NHQEFLPHPQAPQPIVPGLSVVAGASASAAAVASAVAAPAPPQSTTEPLPAMVQTLPLGANSVLTNNPTITITPTPNTAI       560
LQSSLVMGEQNLQWILNGATSSPQNQEQIQQASKVEKVFFTTAVPVASSPGSSVQQIGLSVPVIIIKQEEACQCQCACRD       640
SAKERASSRRKGCSSPPPPEPSPQAPDGPSLQLPAQTFSSAPVPGSSSSTLPSSCEQSRQAETPSDPQTETLSAMDVSEF       720
LSLQSLDTPSNLIPIEALLQGEEEMGLTSSFSK
................................................................................        80
................................................................................       160
................................................................................       240
................................................................................       320
...............aaMaaMaMM........................................................       400
................................................................................       480
................................................................................       560
................................................................................       640
................................................................................       720
.................................
//
NES-accession:	NES-0020
Date:		14 Aug 2002
Protein: 	Heat shock protein SSB1 (Cold-inducible protein YG101) (Ssb1)
Organism:	Saccharomyces cerevisiae (Baker's yeast)
DB_reference: 	SWISS-PROT P11484, HS75_YEAST
Necessity: 	Delta 546-613 in full-length context leads to whole cell
		distribution
Sufficiency: 	Aa 546-99 mediates complete export of GFP-protein A fusion.
		Export mediated by aa 546-86 is less efficient. Delta 574-87
		in a 545-612 context abrogates export
Pathway: 	Crm-dependent
Location: 	Cytoplasmic
Regulation: 	-
Comments: 	-
References:	Shulga N, James P, Craig EA and Goldfarb DS, 1999
		J Biol Chem 274: 16501-7. Medline: 10347213
Sequence:	612 aa   
AEGVFQGAIGIDLGTTYSCVATYESSVEIIANEQGNRVTPSFVAFTPEERLIGDAAKNQAALNPRNTVFDAKRLIGRRFD      80
DESVQKDMKTWPFKVIDVDGNPVIEVQYLEETKTFSPQEISAMVLTKMKEIAEAKIGKKVEKAVITVPAYFNDAQRQATK     160
DAGAISGLNVLRIINEPTAAAIAYGLGAGKSEKERHVLIFDLGGGTFDVSLLHIAGGVYTVKSTSGNTHLGGQDFDTNLL     240
EHFKAEFKKKTGLDISDDARALRRLRTAAERAKRTLSSVTQTTVEVDSLFDGEDFESSLTRARFEDLNAALFKSTLEPVE     320
QVLKDAKISKSQIDEVVLVGGSTRIPKVQKLLSDFFDGKQLEKSINPDEAVAYGAAVQGAILTGQSTSDETKDLLLLDVA     400
PLSLGVGMQGDMFGIVVPRNTTVPTIKRRTFTTCADNQTTVQFPVYQGERVNCKENTLLGEFDLKNIPMMPAGEPVLEAI     480
FEVDANGILKVTAVEKSTGKSSNITISNAVGRLSSEEIEKMVNQAEEFKAADEAFAKKHEARQRLESYVASIEQTVTDPV     560
LSSKLKRGSKSKIEAALSDALAALQIEDPSADELRKAEVGLKRVVTKAMSSR
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
.............aaaaaaaaaaaaaa.........................
//
NES-accession:	NES-0021
Date:		14 Aug 2002
Protein: 	RNA export factor GLE1
Organism:	Saccharomyces cerevisiae (Baker's yeast)
DB_reference: 	SWISS-PROT Q12315, GLE1_YEAST. NLSdb NLS:270
Necessity: 	Delta 351-58 is inviable. Each of the mutantions L351A, L353A, 
		L356A, and L358A lead to inhibition of poly(a)+ RNA export
Sufficiency: 	Aa 350-58 mediates export of HSA (human serum albumin). Each of 
		the above-mentioned mutants abrogates export
Pathway: 	-
Location: 	Predominantly at nuclear pore complexes
Regulation: 	-
Comments: 	-
References:	Murphy R and Wente SR, 1996
		Nature 383: 357-60. Medline: 8848052
Sequence:	538 aa   
MRFVFDEVFNSDTDSPEFEETCSTTSSTSSQCPTPEPSPAIKLPSFTKVGTKKLVNESVVILDPALENALRDLNLQSKLI      80
PINEPIVAASSIIVPHSTNMPLPRASHSSLLDNAKNSNATAPLLEAIEESFQRKMQNLVLANQKEIQSIRENKRRVEEQR     160
KRKEEEERKRKEAEEKAKREQELLRQKKDEEERKRKEAEAKLAQQKQEEERKKIEEQNEKERQLKKEHEAKLLQQKDKLG     240
KAVTNFDKISKMFWHYKDKIAQIKQDIVLPIKKADVNVRNLLSRHKRKINPKFGQLTNSNQQLFKIQNELTQLINDTKGD     320
SLAYHWILNFIAKAVVHQAETEVRVKPESALPLGKLTLYLLVQFPELQELFMARLVKKCPFVIGFTCEIDTEKGRQNMGW     400
KRNNENKWEDNTSYDERMGGILSLFAIITRLQLPQEFITTTSHPFPIALSWHILARICNTPLNLITNTHFVILGSWWDAA     480
AVQFLQAYGNQASKLLILIGEELTSRMAEKKYVGAARLRILLEAWQNNNMESFPEMSP
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
.............................aMaMaaMaM..........................................     400
................................................................................     480
..........................................................
//
NES-accession:	NES-0022
Date:		14 Aug 2002
Protein: 	G2/mitotic-specific cyclin B1
Organism:	Xenopus laevis (African clawed frog)
DB_reference: 	SWISS-PROT P13350, CGB1_XENLA
Necessity: 	F112A in full-length context severely impaires export.
Sufficiency:	Aa 76-125 mediates export of GST.
Pathway: 	Competition by PKI-NES, Crm-interaction, LMB-sensitivity.
Location: 	Cytoplasmic during interphase.
Regulation: 	Possibly by phosphorylation.
Comments: 	-
References:	Yang J, Bardes ES, Moore JD, Brennan J, Powers MA and 
		Kornbluth S, 1998
		Genes Dev 12: 2131-43. Medline: 9679058
Sequence:	397 aa   
MSLRVTRNMLANAENNVKTTLAGKRVVATKPGLRPRTALGDIGNKAEVKVPTKKELKPAVKAAKKAKPVDKLLEPLKVIE      80
ENVCPKPAQVEPSSPSPMETSGCLPDELCQAFSDVLIHVKDVDADDDGNPMLCSEYVKDIYAYLRSLEDAQAVRQNYLHG     160
QEVTGNMRAILIDWLVQVQMKFRLLQETMFMTVGIIDRFLQEHPVPKNQLQLVGVTAMFLAAKYEEMYPPEIGDFTFVTD     240
HTYTKAQIRDMEMKILRVLKFAIGRPLPLHFLRRASKIGEVTAEQHSLAKYLMELVMVDYDMVHFTPSQIAAASSCLSLK     320
ILNAGDWTPTLHHYMAYSEEDLVPVMQHMAKNIIKVNKGLTKHLTVKNKYASSKQMKISTIPQLRSDVVVEMARPLM
...........................................................................aaaaa      80
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaaaaaaaaaaaa...................................     160
................................................................................     240
................................................................................     320
.............................................................................
//
NES-accession:	NES-0023
Date:		14 Aug 2002
Protein: 	Period circadian protein 1 (Circadian pacemaker protein Rigui)
		(mPER) (M-Rigui)
Organism:	Mus musculus (Mouse)
DB_reference: 	SWISS-PROT O35973, PER1_MOUSE
Necessity: 	I493A/L496A abrogates export of full-length PER1.
Sufficiency: 	Delta 1-484 is excluded from the nucleus, whereas delta 1-495
		has a hole cell distribution. Aa 485-98 mediates export of 
		YFP(2)-NLS, but export is abrogated by I493A/L496A/L498K.
Pathway: 	LMB-sensitive
Location: 	-
Regulation: 	Phosphorylation by casein kinase I epsilon masks NLS and results 
		in cytoplasmic accumulation.<
Comments: 	-
References:	Vielhaber EL, Duricka D, Ullman KS and Virshup DM, 2001
		J Biol Chem 276: 45921-7. Medline: 11591712
Sequence:	1291 aa  
MSGPLEGADGGGDPRPGEPFCPGGVPSPGAPQHRPCPGPSLADDTDANSNGSSGNESNGPESRGASQRSSHSSSSGNGKD      80
SALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMTALRELKLRLPPERRGKGRSG     160
TLATLQYALACVKQVQANQEYYQQWSLEEGEPCAMDMSTYTLEELEHITSEYTLRNQDTFSVAVSFLTGRIVYISEQAGV     240
LLRCKRDVFRGARFSELLAPQDVGVFYGSTTPSRLPTWGTGTSAGSGLKDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTP     320
YVTKIRVSDGAPAQPCCLLIAERIHSGYEAPRIPPDKRIFTTRHTPSCLFQDVDERAAPLLGYLPQDLLGAPVLLFLHPE     400
DRPLMLAIHKKILQLAGQPFDHSPIRFCARNGEYVTMDTSWAGFVHPWSRKVAFVLGRHKVRTAPLNEDVFTPPAPSPAP     480
SLDSDIQELSEQIHRLLLQPVHSSSPTGLCGVGPLMSPGPLHSPGSSSDSNGGDAEGPGPPAPVTFQQICKDVHLVKHQG     560
QQLFIESRAKPPPRPRLLATGTFKAKVLPCQSPNPELEVAPVPDQASLALAPEEPERKETSGCSYQQINCLDSILRYLES     640
CNIPSTTKRKCASSSSYTASSASDDDKQRAGPVPVGAKKDPSSAMLSGEGATPRKEPVVGGTLSPLALANKAESVVSVTS     720
QCSFSSTIVHVGDKKPPESDIIMMEDLPGLAPGPAPSPAPSPTVAPDPTPDAYRPVGLTKAVLSLHTQKEEQAFLNRFRD     800
LGRLRGLDTSSVAPSAPGCHHGPIPPGRRHHCRSKAKRSRHHHHQTPRPETPCYVSHPSPVPSSGPWPPPPATTPFPAMV     880
QPYPLPVFSPRGGPQPLPPAPTSVSPATFPSPLVTPMVALVLPNYLFPTPPSYPYGVSQAPVEGPPTPASHSPSPSLPPP     960
PLSPPHRPDSPLFNSRCSSPLQLNLLQLEESPRTEGGAAAGGPGSSAGPLPPSEETAEPEARLVEVTESSNQDALSGSSD    1040
LLELLLQEDSRSGTGSAASGSLGSGLGSGSGSGSHEGGSTSASITRSSQSSHTSKYFGSIDSSEAEAGAARARTEPGDQV    1120
IKCVLQDPIWLLMANADQRVMMTYQVPSRDAASVLKQDRERLRAMQKQQPRFSEDQRRELGAVHSWVRKGQLPRALDVMA    1200
CVDCGSSVQDPGHSDDPLFSELDGLGLEPMEEGGGEGGGCGVGGGGGDGGEEAQTQIGAKGSSSQDSAMEEEEQGGGSSS    1280
PALPAEENSTS
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
....aaaaaaaaMaaMaM..............................................................     560
................................................................................     640
................................................................................     720
................................................................................     800
................................................................................     880
................................................................................     960
................................................................................    1040
................................................................................    1120
................................................................................    1200
................................................................................    1280
...........
//
NES-accession:	NES-0024
Date:		14 Aug 2002
Protein: 	1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 
		delta 1 (PLC-delta-1) (Phospholipase C-delta-1) (PLC-III)
Organism:	Rattus norvegicus (Rat)
DB_reference: 	SWISS-PROT P10688, PID1_RAT 
Necessity: 	L172A/I174A greatly impairs export of full-length protein.
Sufficiency: 	Aa 164-77 mediates export of ovalbumin, whereas the mutant
		peptide L165A/L169A/L172A/LI174A does not.
Pathway: 	LMB-sensitive.
Location: 	Predominantly at the plasma membrane.
Regulation: 	Proposal of calciumregulated mechanism based on structural 
		comparison with EF-hand structure.
Comments: 	NES located in EF-hand. 
References:	Yamaga M, Fujii M, Kamata H, Hirata H and Yagisawa H, 1999
		J Biol Chem 274: 28537-41. Medline: 10497218
Sequence:	756 aa   
MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHR      80
TEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVQGLRKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKM     160
NFKELKDFLKELNIQVDDGYARKIFRECDHSQTDSLEDEEIETFYKMLTQRAEIDRAFEEAAGSAETLSVERLVTFLQHQ     240
QREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMDQPLSHYLVSSSHNTYLLEDQL     320
TGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRV     400
MARHLRAILGPILLDQPLDGVTTSLPSPEQLKGKILLKGKKLGGLLPAGGENGSEATDVSDEVEAAEMEDEAVRSQVQHK     480
PKEDKLKLVPELSDMIIYCKSVHFGGFSSPGTSGQAFYEMASFSESRALRLLQESGNGFVRHNVSCLSRIYPAGWRTDSS     560
NYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPNTTFNSRALTQGPWWRPERLRVRIISGQQ     640
LPKVNKNKNSIVDPKVIVEIHGVGRDTGSRQTAVITNNGFNPRWDMEFEFEVTVPDLALVRFMVEDYDSSSKNDFIGQST     720
IPWNSLKQGYRHVHLLSKNGDQHPSATLFVKISIQD
................................................................................      80
................................................................................     160
...aMaaaMaaMaMaaa...............................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
....................................
//
NES-accession:	NES-0025
Date:		14 Aug 2002
Protein: 	E1B protein, large T-antigen (E1B-55K) 
Organism:	Human adenovirus type 5
DB_reference: 	SWISS-PROT P03243, E1BL_ADE05
Necessity: 	L83A, L87A and L91A abrogates export of full-length protein.
Sufficiency:	Aa 83-93 mediates export of GST-GFP fusion.
Pathway: 	LMB-sensitive.
Location: 	Cytoplasmic.
Regulation: 	-
Comments: 	-
References:	Kratzer F, Rosorius O, Heger P, Hirschmann N, Dobner T,
		Hauber J and Stauber RH, 2000,
		Oncogene 19: 850-7. Medline: 10702793
Sequence:	496 aa   
MERRNPSERGVPAGFSGHASVESGCETQESPATVVFRPPGDNTDGGAAAAAGGSQAAAAGAEPMEPESRPGPSGMNVVQV      80
AELYPELRRILTITEDGQGLKGVKRERGACEATEEARNLAFSLMTRHRPECITFQQIKDNCANELDLLAQKYSIEQLTTY     160
WLQPGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAEVEIDTEDRVAFRCSMINMWPGVLGMDGVVIMNVR     240
FTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWTDVRVRGCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNS     320
RVRHNVASDCGCFMLVKSVAVIKHNMVCGNCEDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGNR     400
RGVFLPYQCNLSHTKILLEPESMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGGKHIRNQPVMLDVTEELRPDHLV     480
LACTRAEFGSSDEDTD
................................................................................      80
..MaaaMaaaMaa...................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................
//
NES-accession:	NES-0026
Date:		14 Aug 2002
Protein: 	38 kDa MOV34 ISOLOGUE (Kip1 C-terminus interacting protein-2) 
		(Jab1/CSN5)
Organism:	Mus musculus (Mouse)
DB_reference: 	TrEMBL O35864
Necessity: 	L237A/L240A/L241A abrogates export.
Sufficiency: 	Aa 233-242 is suficient to interact with Crm in vitro,
		the interaction being disrupted by L237A/L240A/L241A.
Pathway: 	LMB-sensitive, Crm-interaction. 
Location: 	Whole cell distribution.
Regulation: 	-			
Comments: 	-
References:	Tomoda K, Kubota Y, Arata Y, Mori S, Maeda M, Tanaka T, 
		Yoshida M, Yoneda-Kato N and Kato JY, 2002
		J Biol Chem 277: 2302-10. Medline: 11704659
Sequence:	334 aa    
MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGL        80
MLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQ       160
FQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLEL       240
LWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMS       320
QVIKDKLFNQINVA
................................................................................        80
................................................................................       160
........................................................................aaaaMaaM       240
Ma..............................................................................       320
..............
//
NES-accession:	NES-0027
Date:		14 Aug 2002
Protein: 	Tristetraproline (TTP) (TIS11A protein) (TIS11) (ZFP-36)
Organism:	Rattus norvegicus (Rat)
DB_reference: 	SWISS-PROT P47973, TTP_RAT 
Necessity: 	Delta 1-15 and L3A/I6A/L10A both abrogate export of 
		aa 1-161.
Sufficiency:	Aa 1-101 mediates export of GFP.
Pathway: 	LMB sensitive.
Location: 	Whole cell distribution.
Regulation: 	-
Comments: 	-
References:	Murata T, Yoshino Y, Morita N and Kaneda N, 2002
		Biochem Biophys Res Commun 293: 1242-7. Medline: 12054509
Sequence:	320 aa   
MDLSAIYESLMSMSHDLSPDHGGTESSGGLWNINSSDSIPSGVTSRLTGRSTSLVEGRSCSWVPPPPGFAPLAPRPGPEL      80
SPSPTSPTATPTTSSRYKTELCRTYSESGRCRYGAKCQFAHGPGELRQANRHPKYKTELCHKFYLQGRCPYGSRCHFIHN     160
PTEDLALPGQPHVLRQSISFSGLPSGRRTSPPPPGFSGPSLSSCSFSPSSSPPPPGDLPLSPSAFSAAPGTPVSRRDPTP     240
ACCPSCRRSTTPSTIWGPLGGLARSPSAHSLGSDPDDYASSGSSLGGSDSPVFEAGVFGPPQPPAPPRRLPIFNRISVSE
aaMaaMaaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa      80
aaaaaaaaaaaaaaaaaaaaa...........................................................     160
................................................................................     240
................................................................................
//
NES-accession:	NES-0028
Date:		14 Aug 2002
Protein: 	NF-kappaB inhibitor alpha 
		(Major histocompatibility complex enhancer- binding protein MAD3)
		(I-kappa-B-alpha) (IkappaBalpha) (IKB-alpha)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT P25963, IKBA_HUMAN
Necessity: 	I52A/L54A abrogates export of full-length I-kappa-B-alpha
		fused to GST.
Sufficiency: 	Aa 22-72 restores rev-delta-NES acitivity (export).
Pathway: 	LMB-sensitive.
Location: 	Cytoplasmic.
Regulation: 	-
Comments: 	-
References:	Johnson C, Van Antwerp D and Hope TJ, 1999	
		EMBO J 18: 6682-93. Medline: 10581242
Sequence:	317 aa   
MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHL      80
AIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASV     160
GVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCG     240
ADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
.....................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaMaaaaaaaaaaaaaaaaaa........      80
................................................................................     160
................................................................................     240
.............................................................................
//
NES-accession:	NES-0029
Date:		14 Aug 2002
Protein: 	Ran-specific GTPase-activating protein 
		(Ran binding protein 1) (RanBP1)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT P43487, RANG_HUMAN
Necessity: 	L184A/L187A/V189A abrogates export of full-length RanBP1-GFP
Sufficiency: 	Aa 178-95 can substitute Rev-NES
Pathway: 	Rev competition (same pathway). Plafker et al. 2000 has
		furthermore shown LMB-sensitivity.
Location: 	Cytoplasmic.
Regulation: 	-
Comments: 	Discrepancy of 1 in amino acid numbering between article and
		retrieved sequence.
References:	Zolotukhin AS and Felber BK, 1997
		J Biol Chem 1997 272: 11356-60. Medline: 9111043
		Plafker K, Macara IG, 2000
		Mol Cell Biol 20: 3510-21. Medline: 10779340
Sequence:	201 aa   
MAAAKDTHEDHDTSTENTDESNHDPQFEPIVSLPEQEIKTLEEDEEELFKMRAKLFRFASENDLPEWKERGTGDVKLLKH      80
KEKGAIRLLMRRDKTLKICANHYITPMMELKPNAGSDRAWVWNTHADFADECPKPELLAIRFLNAENAQKFKTKFEECRK     160
EIEEREKKAGSGKNDHAEKVAEKLEALSVKEETKEDAEEKQ
................................................................................      80
................................................................................     160
.................aaaaaaMaaMaMaaaaaa......
//
NES-accession:	NES-0030
Date:		14 Aug 2002
Protein: 	Interferon regulatory factor 3 (IRF-3)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT Q14653, IRF3_HUMAN
Necessity: 	I139A/L140A/L143A/L144A abrogates export of full-length IRF-3.
Sufficiency: 	Aa 136-150 mediates export of ovalbumin. Export abrogated by 
		I139A/L140A/L143A/L144A/M147A/V148A/L149A.
Pathway: 	LMB-sensitive (data not shown).
Location: 	Cytoplasmic.
Regulation:	Nuclear upon infection with nexcastle disease virus (NDV).
Comments: 	-
References:	Yoneyama M, Suhara W, Fukuhara Y, Fukuda M, Nishida E 
		and Fujita T, 1998
		EMBO J 17: 1087-95. Medline: 9463386
Sequence:	427 aa   
MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNF      80
RSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSL     160
AVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPG     240
WPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVF     320
DLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHP     400
LSLTSDQYKAYLQDLVEGMDFQGPGES
................................................................................      80
.......................................................aaaMMaaMMaaaaaa..........     160
................................................................................     240
................................................................................     320
................................................................................     400
...........................
//
NES-accession:	NES-0031
Date:		14 Aug 2002
Protein: 	Tumor protein p73 (p53-like transcription factor)
		(p53-related protein)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT O15350, P73_HUMAN. NLSdb NLS:270
Necessity: 	L375A/L377A abrogates export of full-length p73. 
Sufficiency: 	Aa 364-82 mediates export of YFP(2). Export abrogated by 
		abovementioned mutations.
Pathway: 	LMB-sensitive.
Location: 	Nuclear.
Regulation: 	-
Comments: 	-
References:	Inoue T, Stuart J, Leno R and Maki CG, 2002
		J Biol Chem 277: 15053-60. Medline: 11847229
Sequence:	636 aa  
MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAA      80
SASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCP     160
IQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPY     240
EPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSA     320
KNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPS     400
HLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSH     480
CTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQL     560
LRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
...........................................aaaaaaaaaaaMaMaaaaa..................     400
................................................................................     480
................................................................................     560
............................................................................
//
NES-accession:	NES-0032
Date:		14 Aug 2002
Protein:	MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 1 (SMAD 1) 
		(MOTHERS AGAINST DPP HOMOLOG 1) (MAD-RELATED PROTEIN 1)
		(TRANSFORMING GROWTH FACTOR- BETA SIGNALING PROTEIN-1) 
		(BSP-1) (HSMAD1) (JV4-1)
Organism:	Homo sapiens (Human)		
DB_reference:	SWISS-PROT Q15797, SMA1_HUMAN 
Necessity:	L406A/M409A abrogates export.
Sufficiency: 	Aa 406-14 mediates export of GST. Export is abrogated by
		L406A/M409A.
Pathway:	LMB-sensitivity, Crm-dependency.
Location:	Whole cell distribution.
Regulation:	-
Comments:	-
References:	Xiao Z, Watson N, Rodriguez C and Lodish HF, 2001
		J Biol Chem 276: 39404-10. Medline: 11509558
Sequence:	465 aa   
MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHR      80
KGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLG     160
QNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ     240
PMDTNMMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRN     320
STIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGF     400
ETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
.....MaaMaaaaa...................................................
//
NES-accession:	NES-0033
Date:		14 Aug 2002
Protein:	DUAL SPECIFICITY PROTEIN PHOSPHATASE 1 (MITOGEN-ACTIVATED 
		PROTEIN KINASE PHOSPHATASE 7) (MAP KINASE PHOSPHATASE 7) (MKP-7)
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT Q9BY84, DUSX_HUMAN 
Necessity:	L380A/L383A/L385A abrogates export.
Sufficiency:	-
Pathway:	LMB-sensitive.
Location:	Cytoplasmic.
Regulation:	-
Comments:	-
References:	Masuda K, Shima H, Watanabe M and Kikuchi K, 2001,
		J Biol Chem 276: 39002-11. Medline: 11489891
Sequence:	665 aa 		  
MAHEMIGTQIVTERLVALLESGTEKVLLIDSRPFVEYNTSHILEAININCSKLMKRRLQQDKVLITELIQHSAKHKVDID        80
CSQKVVVYDQSSQDVASLSSDCFLTVLLGKLEKSFNSVHLLAGGFAEFSRCFPGLCEGKSTLVPTCISQPCLPVANIGPT       160
RILPNLYLGCQRDVLNKELMQQNGIGYVLNASNTCPKPDFIPESHFLRVPVNDSFCEKILPWLDKSVDFIEKAKASNGCV       240
LVHCLAGISRSATIAIAYIMKRMDMSLDEAYRFVKEKRPTISPNFNFLGQLLDYEKKIKNQTGASGPKSKLKLLHLEKPN       320
EPVPAVSEGGQKSETPLSPPCADSATSEAAGQRPVHPASVPSVPSVQPSLLEDSPLVQALSGLHLSADRLEDSNKLKRSF       400
SLDIKSVSYSASMAASLHGFSSSEDALEYYKPSTTLDGTNKLCQFSPVQELSEQTPETSPDKEEASIPKKLQTARPSDSQ       480
SKRLHSVRTSSSGTAQRSLLSPLHRSGSVEDNYHTSFLFGLSTSQQHLTKSAGLGLKGWHSDILAPQTSTPSLTSSWYFA       560
TESSHFYSASAIYGGSASYSAYSCSQLPTCGDQVYSVRRRQKPSDRADSRRSWHEESPFEKQFKRRSCQMEFGESIMSEN       640
RSREELGKVGSQSSFSGSMEIIEVS
................................................................................        80
................................................................................       160
................................................................................       240
................................................................................       320
...........................................................M..M.M...............       400
................................................................................       480
................................................................................       560
................................................................................       640
.........................
//
NES-accession:	NES-0034
Date:		14 Aug 2002
Protein:	PROTEIN KINASE C, IOTA TYPE (EC 2.7.1.-) (NPKC-IOTA)
		(PROTEIN KINASE C LAMBDA)
Organism:	Mus musculus (Mouse)
DB_reference:	SWISS-PROT Q62074, KPCI_MOUSE 
Necessity:	F244A/L246A mutant accumulates in the nucleus.
Sufficiency:	-
Pathway:	LMB-sensitive.
Location:	Predominantly cytoplasmic.
Regulation:	-
Comments:	Discrepancy of 9 in amino acid numbering between article
		and retrieved sequence. 
References:	Perander M, Bjorkoy G and Johansen T, 2001,
		J Biol Chem 276: 13015-24. Medline: 11115515
Sequence:	586 aa   
MSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKWIDEEGDPCTVSSQLELEEA      80
FRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGY     160
KCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQTMHPDHTQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLG     240
LQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFF     320
VIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTT     400
STFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAA     480
SVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDI     560
VRKIDQSEFEGFEYINPLLMSAEECV
................................................................................      80
................................................................................     160
................................................................................     240
...M.M..........................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
..........................
//
NES-accession:	NES-0035
Date:		14 Aug 2002
Protein:	MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 4 (SMAD 4) (MOTHERS 
		AGAINST DPP HOMOLOG 4) (DELETION TARGET IN PANCREATIC 
		CARCINOMA 4) (HSMAD4)
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT Q13485, SMA4_HUMAN
Necessity:	L146A/L148A abrogates export.
Sufficiency:	-
Pathway:	LMB-sensitive.
Location:	Cytoplasmic.
Regulation:	Export inhibited by protein-protein interactions.
Comments:	-
References:	Watanabe M, Masuyama N, Fukuda M and Nishida E, 2000	
		EMBO Rep 1: 176-82. Medline: 11265759
Sequence:	552 aa   
MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDG      80
RLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKD     160
EYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQI     240
ASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAP     320
EYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVR     400
CLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAP     480
AISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD
................................................................................      80
.................................................................M.M............     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
........................................................................
//
NES-accession:	NES-0036
Date:		14 Aug 2002
Protein:	ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN) 
		(TRIPARTITE MOTIF PROTEIN 27)	
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT P14373, RFP_HUMAN
Necessity:	Aa 132-255 necessary in a deletional mutant context. 
		L204A/L207A/L209A abrogates export.
Sufficiency:	-
Pathway:	LMB-sensitive.
Location:	-
Regulation:	NES activated upon PKC alfa activation, but RFP 
		is not a direct substrate of the kinase.
Comments:	-
References:	Harbers M, Nomura T, Ohno S, Ishii S, 2001,
		J Biol Chem 276: 48596-607. Medline: 11591718
Sequence:	513 aa   
MASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQ      80
LRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRR     160
RAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQ     240
QQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTL     320
DPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTS     400
APQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSG     480
GKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP
................................................................................      80
................................................................................     160
...........................................M..M.M...............................     240
................................................................................     320
................................................................................     400
................................................................................     480
.................................
//
NES-accession:	NES-0037
Date:		14 Aug 2002
Protein:	RPF1/Nedd4, Ubiquitin-protein ligase (E3)
Organism:	Homo sapiens (Human)
DB_reference:	REFSEQ XP_046129.4 
Necessity:	Aa 293-309 necessary in a deletional mutant context. 
		L305A/I307A leads to whole cell distribution.
Sufficiency:	-
Pathway:	LMB-sensitive. 
Location:	Cytoplasmic.
Regulation:	Constitutive export.
Comments:	There is no SWISS-PROT entry  matching this sequence: 
		The swissprot entry P46934 is stated to be only a fragment, but 
		is longer than the sequence used in the article. The XP_046129 
		protein though, match the length and residue numbering of the 
		sequence in the article.
References:	Hamilton MH, Tcherepanova I, Huibregtse JM
		and McDonnell DP, 2001,
		J Biol Chem 276: 26324-31. Medline: 11342538
Sequence:	900 aa   
MATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRV        80
HPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDD       160
NAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQA       240
QRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSS       320
NHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQ       400
SQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWE       480
ERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRI       560
MGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGR       640
VAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGS       720
EIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSA       800
NHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFE       880
ELWDKLQMAIENTQGFDGVD
................................................................................        80
................................................................................       160
................................................................................       240
................................................................M.M.............       320
................................................................................       400
................................................................................       480
................................................................................       560
................................................................................       640
................................................................................       720
................................................................................       800
................................................................................       880
....................
//
NES-accession:	NES-0038
Date:		14 Aug 2002
Protein:	TRANSCRIPTIONAL ACTIVATOR PDR4 (YAP-1 PROTEIN) (Yap1p)
Organism:	Saccharomyces cerevisiae (Baker's yeast)	
DB_reference: 	SWISS-PROT P19880, PDR4_YEAST
Necessity:	V616A, L619A and C620T abrogates export, whereas C620A does not.
Sufficiency: 	-
Pathway:	Crm-dependence and interaction with Crm both in vitro and in
		vivo.
Location:	Cytoplasm.	
Regulation:	Yap1p responds to oxidative stress. NES masked upon oxidation of
		cysteines in the NES region.
Comments:	-
References:	Yan C, Lee LH and Davis LI, 1998,
		EMBO J 17: 7416-29. Medline: 9857197
Sequence:	650 aa   
MSVSTAKRSLDVVSPGSLAEFEGSKSRHDEIENEHRRTGTRDGEDSEQPKKKGSKTSKKQDLDPETKQKRTAQNRAAQRA      80
FRERKERKMKELEKKVQSLESIQQQNEVEATFLRDQLITLVNELKKYRPETRNDSKVLEYLARRDPNLHFSKNNVNHSNS     160
EPIDTPNDDIQENVKQKMNFTFQYPLDNDNDNDNSKNVGKQLPSPNDPSHSAPMPINQTQKKLSDATDSSSATLDSLSNS     240
NDVLNNTPNSSTSMDWLDNVIYTNRFVSGDDGSNSKTKNLDSNMFSNDFNFENQFDEQVSEFCSKMNQVCGTRQCPIPKK     320
PISALDKEVFASSSILSSNSPALTNTWESHSNITDNTPANVIATDATKYENSFSGFGRLGFDMSANHYVVNDNSTGSTDS     400
TGSTGNKNKKNNNNSDDVLPFISESPFDMNQVTNFFSPGSTGIGNNAASNTNPSLLQSSKEDIPFINANLAFPDDNSTNI     480
QLQPFSESQSQNKFDYDMFFRDSSKEGNNLFGEFLEDDDDDKKAANMSDDESSLIKNQLINEEPELPKQYLQSVPGNESE     560
ISQKNGSSLQNADKINNGNDNDNDNDVVPSKEGSLLRCSEIWDRITTHPKYSDIDVDGLCSELMAKAKCSERGVVINAED     640
VQLALNKHMN
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
.......................................................M..MM....................     640
..........
//
NES-accession:	NES-0039
Date:		14 Aug 2002
Protein:	Putative ATP-dependent RNA helicase An3
Organism:	Xenopus laevis (African clawed frog)
DB_reference: 	SWISS-PROT P24346, AN3_XENLA
Necessity:	Delta 1-21 and L19A/L21A both abrogates export.
Sufficiency: 	 -
Pathway:	Full-length An3 interacts in vitro with Crm1, whereas delta
		1-21 aa does not.
Location:	-
Regulation:	-
Comments:	-
References:	Askjaer P, Bachi A, Wilm M, Bischoff FR, Weeks DL, Ogniewski V,
		Ohno M, Niehrs C, Kjems J, Mattaj IW, Fornerod M, 1999,	
		Mol Cell Biol 19: 6276-85. Medline: 10454574
Sequence:	697 aa   
MSHVAVENVLNLDQQFAGLDLNSADAESGVAGTKGRYIPPHLRNKEASRNDSNWDSGRGGNGYINGMQDDRDGRMNGYDR      80
GGYGSRGTGRSDRGFYDRENSGWNSGRDKDAYSSFGSRGDRGKGSLFNERGSGSRRTDDRRQDGFDGMGNRSDKSGFGRF     160
DRGNSRWSDDRNDEDDWSKPLAPNDRVEQELFSGSNTGINFEKYDDIPVEATGSNCPPHIESFHDVTMGEIIMGNIQLTR     240
YTRPTPVQKHAIPIIIEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGDAMKHLQENGRYGRRKQFPLSLVLAPTRELA     320
VQIYEEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEP     400
QIRRIVEQDTMPPKGVRQTMMFSATFPKEIQILARDFLDEYIFLAVGRVGSTSENITQKVVWVEEMDKRSFLLDLLNATG     480
KDSLTLVFVETKKGADALEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLP     560
SDIEEYVHRIGRTGRVGNLGLATSFFNEKNINITKDLLDLLVEAKQEVPSWLENMAYEQHHKSSSRGRSKSRFSGGFGAK     640
DYRQSSGAGSSFGSSRGGRSSGHGGSRGFGGGYGGFYNSDGYGGNYGGSSQVDWWGN
..................M.M...........................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
.........................................................
//
NES-accession:	NES-0040
Date:		14 Aug 2002
Protein:	M-phase inducer phosphatase 1/B (Cdc25)
Organism:	Xenopus laevis (African clawed frog)
DB_reference:	SWISS-PROT P30309, MPI1_XENLA
Necessity:	Both L53A/V55A/F57A and L344A/V347A/I349A greatly impair export
		of full-length fused to GFP.
Sufficiency: 	-
Pathway:	LMB-sensitivity.
Location:	Cytoplasmic.
Regulation:	-
Comments:	Aa 47-57 is a stronger NES than aa 342-50.
References:	Kumagai A and Dunphy WG, 1999
		Genes Dev 13: 1067-72. Medline: 10323858
Sequence:	550 aa  
MAESHIMSSEAPPKTNTGLNFRTNCRMVLNLLREKDCSVTFSPEQPLTPVTDLAVGFSNLSTFSGETPKRCLDLSNLGDE      80
TAPLPTESPDRISSGKVESPKAQFVQFDGLFTPDLGWKAKKCPRGNMNSVLPRLLCSTPSFKKTSGGQRSVSNKENEGEL     160
FKSPNCKPVALLLPQEVVDSQFSPTPENKVDISLDEDCEMNILGSPISADPPCLDGAHDDIKMQNLDGFADFFSVDEEEM     240
ENPPGAVGNLSSSMAILLSGPLLNQDIEVSNVNNISLNRSRLYRSPSMPEKLDRPMLKRPVRPLDSETPVRVKRRRSTSS     320
SLQPQEENFQPQRRGTSLKKTLSLCDVDISTVLDEDCGHRQLIGDFTKVYALPTVTGRHQDLRYITGETLAALIHGDFSS     400
LVEKIFIIDCRYPYEYDGGHIKGALNLHRQEEVTDYFLKQPLTPTMAQKRLIIIFHCEFSSERGPKMCRFLREEDRARNE     480
YPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPMHHQDFREELLKFRTKCKTSVGDRKRREQIARIMKL
....................................................M.M.M.......................      80
................................................................................     160
................................................................................     240
................................................................................     320
.......................M..M.M...................................................     400
................................................................................     480
......................................................................
//
NES-accession:	NES-0041
Date:		14 Aug 2002
Protein: 	Adenomatous polyposis coli protein (APC protein)
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT P25054, APC_HUMAN
Necessity: 	Export of either full-length APC or a VP16-NLS-aa1-270 fusion 
		protein, both get abrogated by simultaneous mutation of both 
		NESs (L75A/L77A/L172A/L174A).
Sufficiency:	Aa 68-77 and 165-74 are each sufficient to mediate 
		export of GST, aa 68-77 being a stronger NES than 165-74.
Pathway:	LMB-sensitivity: In vivo interaction with Crm is dependent 
		on the intact NES sequences.
Location: 	Cytoplasmic.
Regulation: 	-
Comments:	2 NESs.
References: 	Neufeld KL, Nix DA, Bogerd H, Kang Y, Beckerle MC,
		Cullen BR and White RL, 2000,
		Proc Natl Acad Sci U S A 97: 12085-90. Medline: 11035805
Sequence:	2843 aa  
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSS      80
NFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYA     160
QLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRVAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRIRQLLQSQAT     240
EAERSSQNKHETGSHDAERQNEGQGVGEINMATSGNGQGSTTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMVYSLLS     320
MLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRG     400
RREIRVLHLLEQIRAYCETCWEWQEAHEPGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQ     480
VDCEMYGLTNDHYSITLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSK     560
KTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILR     640
NVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAM     720
GSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQSLYGDYV     800
FDTNRHDDNRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLGNYHPATENPGTSSKRGLQI     880
STTAAQIAKVMEEVSAIHTSQEDRSSGSTTELHCVTDERNALRRSSAAHTHSNTYNFTKSENSNRTCSMPYAKLEYKRSS     960
NDSLNSVSSSDGYGKRGQMKPSIESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGR    1040
QSPSQNERWARPKHIIEDEIKQSEQRQSRNQSTTYPVYTESTDDKHLKFQPHFGQQECVSPYRSRGANGSETNRVGSNHG    1120
INQNVSQSLCQEDDYEDDKPTNYSERYSEEEQHEEEERPTNYSIKYNEEKRHVDQPIDYSLKYATDIPSSQKQSFSFSKS    1200
SSGQSSKTEHMSSSSENTSTPSSNAKRQNQLHPSSAQSRSGQPQKAATCKVSSINQETIQTYCVEDTPICFSRCSSLSSL    1280
SSAEDEIGCNQTTQEADSANTLQIAEIKEKIGTRSAEDPVSEVPAVSQHPRTKSSRLQGSSLSSESARHKAVEFSSGAKS    1360
PSKSGAQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPP    1440
PPPQTAQTKREVPKNKAPTAEKRESGPKQAAVNAAVQRVQVLPDADTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDV    1520
ELRIMPPVQENDNGNETESEQPKESNENQEKEAEKTIDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKPAQTASK    1600
LPPPVARKPSQLPVYKLLPSQNRLQPQKHVSFTPGDDMPRVYCVEGTPINFSTATSLSDLTIESPPNELAAGEGVRGGAQ    1680
SGEFEKRDTIPTEGRSTDEAQGGKTSSVTIPELDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASASSSAP    1760
NKNQLDGKKKKPTSPVKPIPQNTEYRTRVRKNADSKNNLNAERVFSDNKDSKKQNLKNNSKDFNDKLPNNEDRVRGSFAF    1840
DSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKAKENKESEAKVTSHTELTSNQQSANKTQAIAKQPINR    1920
GQPKPILQKQSTFPQSSKDIPDRGAATDEKLQNFAIENTPVCFSHNSSLSSLSDIDQENNNKENEPIKETEPPDSQGEPS    2000
KPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKKPSRLKGDNEKHSPRNMGGILGEDLTLDL    2080
KDIQRPDSEHGLSPDSENFDWKAIQEGANSIVSSLHQAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFT    2160
SNKGPRILKPGEKSTLETKKIESESKGIKGGKKVYKSLITGKVRSNSEISGQMKQPLQANMPSISRGRTMIHIPGVRNSS    2240
SSTSPVSKKGPPLKTPASKSPSEGQTATTSPRGAKPSVKSELSPVARQTSQIGGSSKAPSRSGSRDSTPSRPAQQPLSRP    2320
IQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQMSQQNLTKQTGLSKNASSIPRSESASKG    2400
LNQMNNGNGANKKVELSRMSSTKSSGSESDRSERPVLVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPASPTRSQAQ    2480
TPVLSPSLPDMSLSTHSSVQAGGWRKLPPNLSPTIEYNDGRPAKRHDIARSHSESPSRLPINRSGTWKREHSKHSSSLPR    2560
VSTWRRTGSSSSILSASSESSEKAKSEDEKHVNSISGTKQSKENQVSAKGTWRKIKENEFSPTNSTSQTVSSGATNGAES    2640
KTLIYQMAPAVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKANPNIKDSKDNQAKQNVGNGSVPMRTVGLEN    2720
RLNSFIQVDAPDQKGTEIKPGQNNPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSA    2800
RPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHSGSYLVTSV
...................................................................aaaaaaaMaM...      80
................................................................................     160
....aaaaaaaMaM..................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
................................................................................     800
................................................................................     880
................................................................................     960
................................................................................    1040
................................................................................    1120
................................................................................    1200
................................................................................    1280
................................................................................    1360
................................................................................    1440
................................................................................    1520
................................................................................    1600
................................................................................    1680
................................................................................    1760
................................................................................    1840
................................................................................    1920
................................................................................    2000
................................................................................    2080
................................................................................    2160
................................................................................    2240
................................................................................    2320
................................................................................    2400
................................................................................    2480
................................................................................    2560
................................................................................    2640
................................................................................    2720
................................................................................    2800
...........................................
//
NES-accession:	NES-0042
Date:		14 Aug 2002
Protein: 	Phosphorylated adaptor for RNA export (PHAX)
Organism:	Mus musculus (Mouse)
DB_reference: 	TrEMBL Q9JJT9
Necessity: 	V120A/L124A/L127A/M129A prevents Crm-association.
Sufficiency:	-
Pathway: 	Crm-complex formation.
Location: 	-
Regulation: 	Exported upon phosphorylation.
Comments: 	-
References:	Ohno M, Segref A, Bachi A, Wilm M and Mattaj IW, 2000,
		Cell 101: 187-98. Medline: 10786834
Sequence:	385 aa    
MALEAGDMEEGQLSDSDSDMTVVPSDRPLQMAKVLGGGSAACAPVSHYRTVKHVDSSEESLDSDDDCSLWKRKRQKCHNT        80
PPKPEPFPFGPSGQKTALNGGKKVNNIWGAVLQEQNQDAVATELGILGMEGSIDRSRQSETYNYLLAKKLAKKESQEYTK       160
ELDKDLDEYMHGDKKPGSKEDENGQGHLKRKRPVRDRLGNRVEMNYKGRYEITEEDAPEKVADEIAFRLQEPKKDLIARV       240
VRILGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYVENQKEYENKKAARKRRTQL       320
LGKKMKQAIKSLNFQEDDDTSRETFASDTNEALASLDEAQEGPGETKLDAEEAIEVDHPQDLDIF
................................................................................        80
.......................................M...M..M.M...............................       160
................................................................................       240
................................................................................       320
.................................................................
//
NES-accession:	NES-0043
Date:		14 Aug 2002
Protein: 	Brain expressed X-linked protein 3 
		(P75NTR-associated cell death EXECUTOR) (NADE)
Organism:	Mus musculus (Mouse)
DB_reference: 	TrEMBL Q9WTZ9
Necessity: 	L94A/L97A/L99A leads to whole cell distribution.
Sufficiency:	-
Pathway: 	LMB-sensitive.
Location: 	Cytoplasmic.
Regulation: 	-
Comments: 	-
References:	Mukai J, Shoji S, Kimura MT, Okubo S, Sano H, Suvanto P, 
		Li Y, Irie S and Sato TA, 2002,
		J Biol Chem 277: 13973-82. Medline: 11830582
Sequence:	124 aa    
MANVHQENEEMEQPLQNGQEDRPVGGGEGHQPAANNNNNNHNHNHNHHRRGQARRLAPNFRWAIPNRQMNDGLGGDGDDM        80
EMFMEEMREIRRKLRELQLRNCLRILMGELSNHHDHHDEFCLMP
................................................................................        80
.............M..M.M.........................
//
NES-accession:	NES-0044
Date:		14 Aug 2002
Protein: 	Open reading frame YDR499w (Cip1p) 
Organism:	Saccharomyces cerevisiae (Baker's yeast)
DB_reference:	REFSEQ NP_010787.1
Necessity: 	L670A/L673A abrogates export
Sufficiency:	Aa 660-747 fragment interacts with Crm in vitro, in a 
		LMB-sensitive manner.
Pathway: 	LMB-sensitive.
Location: 	Whole cell distribution.
Regulation: 	-
Comments: 	Sequence stated as open reading frame YDR499w by the
		authors which corresponds to REFSEQ NP_010787.1. 
		No corresponding SWISS-PROT entry. 
References:	Jensen TH, Neville M, Rain JC, McCarthy T, Legrain P
		and Rosbash M, 2000,
		Mol Cell Biol 20: 8047-58. Medline: 11027275
Sequence:	747 aa    
MRRETVGEFSSDDDDDILLELGTRPPRFTQIPPSSAALQTQIPTTLEVTTTTLNNKQSKNDNQLVNQLNKAQGEASMLRD        80
KINFLNIEREKEKNIQAVKVNELQVKHLQELAKLKQELQKLEDEKKFLQMEARGKSKREVITNVKPPSTTLSTNTNTITP       160
DSSSVAIEAKPQSPQSKKRKISDNLLKKNMVPLNPNRIIPDETSLFLESILLHQIIGADLSTIEILNRLKLDYITEFKFK       240
NFVIAKGAPIGKSIVSLLLRCKKTLTLDRFIDTLLEDIAVLIKEISVHPNESKLAVPFLVALMYQIVQFRPSATHNLALK       320
DCFLFICDLIRIYHHVLKVPIHESNMNLHVEPQIFQYELIDYLIISYSFDLLEGILRVLQSHPKQTYMEFFDENILKSFE       400
FVYKLALTISYKPMVNVIFSAVEVVNIITSIILNMDNSSDLKSLISGSWWRDCITRLYALLEKEIKSGDVYNENVDTTTL       480
HMSKYHDFFGLIRNIGDNELGGLISKLIYTDRLQSVPRVISKEDIGMDSDKFTAPIIGYKMEKWLLKLKDEVLNIFENLL       560
MIYGDDATIVNGEMLIHSSKFLSREQALMIERYVGQDSPNLDLRCHLIEHTLTIIYRLWKDHFKQLREEQIKQVESQLIM       640
SLWRFLVCQTETVTANEREMRDHRHLVDSLHDLTIKDQASYYEDAFEDLPEYIEEELKMQLNKRTGRIMQVKYDEKFQEM       720
ARTILESKSFDLTTLEEADSLYISMGL
................................................................................        80
................................................................................       160
................................................................................       240
................................................................................       320
................................................................................       400
................................................................................       480
................................................................................       560
................................................................................       640
...................aaaaaaaaaaMaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa       720
aaaaaaaaaaaaaaaaaaaaaaaaaaa
//
NES-accession:	NES-0045Date:		14 Aug 2002
Protein: 	Nonstructural protein NS2 
Organism:	Influenza B virus (strain B/Yamagata/1/73)
DB_reference:	SWISS-PROT P08014, VNS2_INBYA
Necessity: 	NEP-B can substitute for HIV1 Rev effector domain, whereas
		M14A/M17A/I19A cannot.		
Sufficiency:	-
Pathway: 	Crm-interaction in 2 hybrid system 
Location: 	-
Regulation: 	-
Comments: 	-
References:	Paragas J, Talon J, O'Neill RE, Anderson DK, Garcia-Sastre A
		and Palese P, 2001,
		J Virol 75: 7375-83. Medline: 11462009
Sequence:	122 aa  
MADNMTTTQIEWRMKKMAIGSSTHSSSVLMKDIQSQFEQLKLRWESYPNLVKSTDYHQRRETIRLVTEELYLLSKRIDDN      80
ILFHKTVIANSSIIADMIVSLSLLETLYEMKDVVEVYSRQCL
.............M..M.M.............................................................      80
..........................................
//
NES-accession:	NES-0046
Date:		14 Aug 2002
Protein: 	Nonstructural protein NS2
Organism:	Influenza A virus (strain A/Puerto Rico/8/34)
DB_reference: 	SWISS-PROT P03508, VNS2_IAPUE
Necessity: 	Fragments containg aa 1-30 can substitute for HIV1 Rev effector 
		domain, whereas fragments lacking aa 1-30 cannot. 
		M16A/M19A/L21A in full-length context abrogates export activity. 
Sufficiency: 	Aa 1-30 can substitue for HIV1 Rev effector domain. 
		Aa 11-23 mediates export of GST, but if containg the above-
		mentioned mutations, export is abrogated.
Pathway: 	-
Location: 	-
Regulation: 	-
Comments: 	-
References:	O'Neill RE, Talon J and Palese P, 1998,
		EMBO J 17: 288-96. Medline: 9427762
Sequence:	121 aa   
MDPNTVSSFQDILLRMSKMQLESSSGDLNGMITQFESLKLYRDSLGEAVMRMGDLHSLQNRNEKWREQLGQKFEEIRWLI      80
EEVRHKLKITENSFEQITFMQALHLLLEVEQEIRTFSFQLI
...............M..M.M...........................................................      80
.........................................
//
NES-accession:	NES-0047
Date:		14 Aug 2002
Protein: 	NF-kappaB inhibitor epsilon (NF-kappa-BIE) (I-kappa-B-epsilon)
		(IkappaBepsilon) (IKB-epsilon) (IKBE)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT O00221, IKBE_HUMAN
Necessity: 	L350A/I352A abrogates export of full-length IKBE.
Sufficiency:	-
Pathway: 	LMB-sensitive, Crm-interaction in vitro.
Location: 	Cytoplasmic.
Regulation:  	-
Comments: 	-
References:	Lee SH and Hannink M, 2002,
		J Biol Chem 277: 23358-66. Medline: 11970947
Sequence:	361 aa    
MSEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASGPSDGSPQPCTHPPGPVKEPQEKEDADGERADSTYGSSSLTY      80
TLSLLGGPEAEDPPPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCCLALLPQEVLDIQNNLYQTALH     160
LAVHLDQPGAVRALVLKGASRALQDRHGDTALHVACQRQSWPVPAACWKGGPEPGRGTSHSLDLQLQNWQGLACLHIATL     240
QKNQPLMELLLRNGADIDVQEGSSGKTALHLAVETQERGLVQFLLQAGAQVDARMLNGCTPLHLAAGRGLMGISSTLCKA     320
GADSLLRNVEDETPQDLTEESLVLLPFDDLKISGKLLLCTD
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
.............................M.M.........
//
NES-accession:	NES-0048
Date:		14 Aug 2002
Protein: 	ATP-dependent RNA helicase p54 (Xp54) (DDX6)
Organism:	Xenopus laevis (African clawed frog)
DB_reference:	SWISS-PROT P54824, DDX6_XENLA
Necessity: 	L157A/L159A abrogates export of aa 1-164 fragment.
Sufficiency:	Aa 1-164 mediates export of GFP.
Pathway: 	LMB-sensitive.
Location: 	Cytoplasmic in quiescent and full-grown oocytes, but nuclear 
		in developing oocytes.
Regulation: 	-
Comments: 	Discrepancy of 1 in amino acid numbering between article and
		retrieved sequence. 
References:	Smillie DA and Sommerville J, 2001,
		 J Cell Sci115: 395-407. Medline: 11839790
Sequence:	481 aa 
MSTARTENPVLMGMSSQNGQLRGPLKPSAGPGGGGTQTQQINQLKNASTINSGSQQQAQSMSSIIKPGDDWKKTLKLPPK      80
DLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKTGAYLIPLLERLDLK     160
KDCIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHIQ     240
MIVLDEADKLLSQDFMQIMEDIIMTLPKNRQILLYSATFPLSVQKFMTLHLQKPYEINLMEELTLKGVTQYYAYVTERQK     320
VHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQ     400
AVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSSIDKNLYVAEYHSESGEDK     480
P
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa      80
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaMa     160
aaaa............................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
.
//
NES-accession:	NES-0049
Date:		14 Aug 2002
Protein:	TELOMERASE REVERSE TRANSCRIPTASE (TELOMERASE CATALYTIC SUBUNIT) 
		(HEST2) (TERT)	
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT O14746, TERT_HUMAN
Necessity:	-	
Sufficiency: 	In vitro Crm-interaction of aa 831-1132 fused to GST was 
		abrogated by L974A/L978A/L980A.
Pathway:	LMB-sensitive. 	
Location:	Cytoplasmic in the absence of 14-3-3, nuclear in the
		presence of 14-3-3.	
Regulation:	NES masked upon binding of 14-3-3 protein.	
Comments:	-
References:	Seimiya H, Sawada H, Muramatsu Y, Shimizu M, Ohko K,
		Yamane K and Tsuruo T, 2000,
		EMBO J 2000 19: 2652-61. Medline: 10835362
Sequence:	1132 aa  
MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKEL      80
VARVLQRLCERGAKNVLAFGFALLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFV     160
LVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARRRGGSASRSLPLPKRPRR     240
GAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTP     320
CPPVYAETKHFLYSSGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNH     400
AQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPWQVYGFVRACLRRLVPPGLWGS     480
RHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSF     560
FYVTETTFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNMDYVV     640
GARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTI     720
PQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNE     800
ASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLLVTPHLTHA     880
KTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTF     960
NRGFKAGRNMRRKLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTA    1040
SLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLPGTTLTALEAAAN    1120
PALPSDFKTILD
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
................................................................................     800
..............................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa     880
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa     960
aaaaaaaaaaaaaMaaaMaMaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa    1040
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa    1120
aaaaaaaaaaaa
//
NES-accession:	NES-0050
Date:		14 Aug 2002
Protein:	Ran GTPase activating protein 1 (Rna1p)
Organism:	Saccharomyces cerevisiae (Baker's yeast)
DB_reference:	SWISS-PROT P11745, RNA1_YEAST
Necessity:	-
Sufficiency: 	Aa 316-57 mediates export of H2B-lacZ fusion. 
		Aa 316-33 and 329-57 each mediates export, aa 329-57 being most
		effective.
		L326A/I328A abrogates export of aa 316-33 fragment.
Pathway:	Crm-dependent
Location:	Cytoplasmic	
Regulation:	-
Comments:	2 overlapping NESs. "S" and "E" in the assignment field,
		describes start and end of fragments shown to have NES activity.
References:	Feng W, Benko AL, Lee JH, Stanford DR and Hopper AK, 1999,
		J Cell Sci 112: 339-47. Medline: 9885287
Sequence:	407 aa    
MATLHFVPQHEEEQVYSISGKALKLTTSDDIKPYLEELAALKTCTKLDLSGNTIGTEASEALAKCIAENTQVRESLVEVN      80
FADLYTSRLVDEVVDSLKFLLPVLLKCPHLEIVNLSDNAFGLRTIELLEDYIAHAVNIKHLILSNNGMGPFAGERIGKAL     160
FHLAQNKKAASKPFLETFICGRNRLENGSAVYLALGLKSHSEGLKVVKLYQNGIRPKGVATLIHYGLQYLKNLEILDLQD     240
NTFTKHASLILAKALPTWKDSLFELNLNDCLLKTAGSDEVFKVFTEVKFPNLHVLKFEYNEMAQETIEVSFLPAMEKGNL     320
PELEKLEINGNRLDEDSDALDLLQSKFDDLEVDDFEEVDSEDEEGEDEEDEDEDEKLEEIETERLEKELLEVQVDDLAER     400
LAETEIK
................................................................................      80
................................................................................     160
................................................................................     240
...........................................................................Saaaa     320
aaaaaMaMSaaaEaaaaaaaaaaaaaaaaaaaaaaaE...........................................     400
.......
//
NES-accession:	NES-0051
Date:		14 Aug 2002
Protein: 	Transcription factor IIIA (Factor A) (TFIIIA)
Organism:	Bufo americanus (American toad)	
DB_reference:	SWISS-PROT P34694, TF3A_BUFAM
Necessity:	-
Sufficiency: 	Aa 320-339 mediates export of BSA in oocytes and of GST in HeLa
		cells. Export abrogated by L336A.	 
Pathway:	Aa 320-339 can substitute for the HIV-1 Rev effector domain.	
Location:	-		
Regulation:	-
Comments:	-
References:	Fridell RA, Fischer U, Luhrmann R, Meyer BE, Meinkoth JL, 
		Malim MH and Cullen BR, 1996,
		Proc Natl Acad Sci U S A 93: 2936-40. Medline: 8610146
Sequence:	339 aa   
MGEKLPVVYKRFICSFPDCNATYNKNRKLQAHLCKHTGERPFPCTYEGCEKGFVTLHHLNRHVLSHTGEKPCKCETENCN      80
LAFTTASNMRLHFKRAHSSPAQVYVCYFADCGQQFRKHNQLKIHQYIHTNQQPFKCSHEGCDKCYASPSRLKRHEKTHAG     160
YPCRKDSTCPFVGKTWSDYMKHAAELHSEVTCSICNRTFKRKSFLKEHKKIHREERIVYRCPRENCDRTYTTKFNLKSHI     240
LTFHENLRPFVCEHEGCGKTFAMKQSLDRHFNTHDPEKKKMVKPPRPVRSLASRLSGYKPKKSKKKKKPSQTPAMESQEQ     320
QPDASKADPLPVLENLTLK
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
aaaaaaaaaaaaaaaMaaa
//
NES-accession:	NES-0052
Date:		14 Aug 2002
Protein:	Transcription factor IIIA (Factor A) (TFIIIA) (S-TFIIIA/O-TFIIIA)
Organism:	Xenopus laevis (African clawed frog)
DB_reference:	SWISS-PROT P03001, TF3A_XENLA
Necessity:	-
Sufficiency: 	Aa 347-66 mediates export of reporterprotein. 
Pathway:		Aa 347-66 can complement for the HIV-1 Rev effector
		domain.  
Location:	-
Regulation:	-
Comments:	-
References:	Fridell RA, Fischer U, Luhrmann R, Meyer BE, Meinkoth JL, 
		Malim MH and Cullen BR, 1996,
		Proc Natl Acad Sci U S A 93: 2936-40. Medline: 8610146
		Ward BM and Lazarowitz SG, 1999,
		Plant Cell 11: 1267-76. Medline: 10402428
Sequence:	366 aa   
MAAKVASTSSEEAEGSLVTEGEMGEKALPVVYKRYICSFADCGAAYNKNWKLQAHLCKHTGEKPFPCKEEGCEKGFTSLH      80
HLTRHSLTHTGEKNFTCDSDGCDLRFTTKANMKKHFNRFHNIKICVYVCHFENCGKAFKKHNQLKVHQFSHTQQLPYECP     160
HEGCDKRFSLPSRLKRHEKVHAGYPCKKDDSCSFVGKTWTLYLKHVAECHQDLAVCDVCNRKFRHKDYLRDHQKTHEKER     240
TVYLCPRDGCDRSYTTAFNLRSHIQSFHEEQRPFVCEHAGCGKCFAMKKSLERHSVVHDPEKRKLKEKCPRPKRSLASRL     320
TGYIPPKSKEKNASVSGTEKTDSLVKNKPSGTETNGSLVLDKLTIQ
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
..........................aaaaaaaaaaaaaaaaaaaa
//
NES-accession:	NES-0053
Date:		14 Aug 2002
Protein:	Ah receptor (Aryl hydrocarbon receptor) (AhR)	
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT P35869, AHR_HUMAN
Necessity:	-
Sufficiency: 	Aa 55-75 mediates export of GFP-GST fusion. Abrogated by
		L70A/L72A.
Pathway:	Export abrogated by competition of the PKI-NES (NES-0001), which
		indicates that AhR gets exported by the same pathway as PKI. 
Location: 	-
Regulation:	-
Comments:	-	
References:	Ikuta T, Eguchi H, Tachibana T, Yoneda Y and Kawajiri K, 1998	
		J Biol Chem 273: 2895-904. Medline: 9446600
Sequence:	848 aa   
MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAK      80
SFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYE     160
LIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQ     240
GKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGS     320
GYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLTDEEGTEHLRKRNT     400
KLPFMFTTGEAVLYEATNPFPAIMDPLPLRTKNGTSGKDSATTSTLSKDSLNPSSLLAAMMQQDESIYLYPASSTSSTAP     480
FENNFFNESMNECRNWQDNTAPMGNDTILKHEQIDQPQDVNSFAGGHPGLFQDSKNSDLYSIMKNLGIDFEDIRHMQNEK     560
FFRNDFSGEVDFRDIDLTDEILTYVQDSLSKSPFIPSDYQQQQSLALNSSCMVQEHLHLEQQQQHHQKQVVVEPQQQLCQ     640
KMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYP     720
MGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLN     800
KFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL
......................................................aaaaaaaaaaaaaaaMaMaaa.....      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
................................................................................     800
................................................
//
NES-accession:	NES-0054
Date:		14 Aug 2002
Protein: 	Period circadian protein 2 (mPER2)
Organism:	Mus musculus (Mouse)
DB_reference: 	SWISS-PROT O54943, PER2_MOUSE
Necessity: 	-
Sufficiency: 	Aa 382-469 mediates export of YFP(2)-NLS. Export is abrogated
		by I464A/L467A/M469K and greatly impaired by M469K alone.
Pathway: 	LMB-sensitive.
Location: 	-
Regulation: 	-
Comments: 	-
References:	Vielhaber EL, Duricka D, Ullman KS and Virshup DM, 2001
		J Biol Chem 276: 45921-7. Medline: 11591712
Sequence:	1257 aa  
MNGYVDFSPSPTSPTKEPGAPQPTQAVLQEDVDMSSGSSGNENCSTGRDSQGSDCDDNGKELRMLVESSNTHPSPDDAFR      80
LMMTEAEHNPSTSGCSSEQSAKADAHKELIRTLKELKVHLPADKKAKGKASTLATLKYALRSVKQVKANEEYYQLLMSSE     160
SQPCSVDVPSYSMEQVEGITSEYIVKNADMFAVAVSLVSGKILYISNQVASIFHCKKDAFSDAKFVEFLAPHDVSVFHSY     240
TTPYKLPPWSVCSGLDSFTQECMEEKSFFCRVSVGKHHENEIRYQPFRMTPYLVKVQEQQGAESQLCCLLLAERVHSGYE     320
APRIPPEKRIFTTTHTPNCLFQAVDERAVPLLGYLPQDLIETPVLVQLHPSDRPLMLAIHKKILQAGGQPFDYSPIRFRT     400
RNGEYITLDTSWSSFINPWSRKISFIIGRHKVRVGPLNEDVFAAPPCPEEKTPHPSVQELTEQIHRLLMQPVPHSGSSGY     480
GSLGSNGSHEHLMSQTSSSDSNGQEESHRRRSGIFKTSGKIQTKSHVSHESGGQKEASVAEMQSSPPAQVKAVTTIERDS     560
SGASLPKASFPEELAYKNQPPCSYQQISCLDSVIRYLESCSEAATLKRKCEFPANIPSRKATVSPGLHSGEAARPSKVTS     640
HTEVSAHLSSLTLPGKAESVVSLTSQCSYSSTIVHVGDKKPQPELETVEDMASGPESLDGAAGGLSQEKGPLQKLGLTKE     720
VLAAHTQKEEQGFLQRFREVSRLSALQAHCQNYLQERSRAQASDRGLRNTSGLESSWKKTGKNRKLKSKRVKTRDSSEST     800
GSGGPVSHRPPLMGLNATAWSPSDTSQSSCPSAPFPTAVPAYPLPVFQAPGIVSTPGTVVAPPAATHTGFTMPVVPMGTQ     880
PEFAVQPLPFAAPLAPVMAFMLPSYPFPPATPNLPQAFLPSQPHFPAHPTLASEITPASQAEFPSRTSTLRQPCACPVTP     960
PAGTVALGRASPPLFQSRGSSPLQLNLLQLEEAPEGSTGAAGTLGTTGTAASGLDCTSGTSRDRQPKAPPTCNEPSDTQN    1040
SDAISTSSDLLNLLLGEDLCSATGSALSRSGASATSDSLGSSSLGFGTSQSGAGSSDTSHTSKYFGSIDSSENNHKAKMI    1120
PDTEESEQFIKYVLQDPIWLLMANTDDSIMMTYQLPSRDLQAVLKEDQEKLKLLQRSQPRFTEGQRRELREVHPWVHTGG    1200
LPTAIDVTGCVYCESEEKGNICLPYEEDSPSPGLCDTSEAKEEEGEQLTGPRIEAQT
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
.............................................................aaaaaaaaaaaaaaaaaaa     400
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaMaM...........     480
................................................................................     560
................................................................................     640
................................................................................     720
................................................................................     800
................................................................................     880
................................................................................     960
................................................................................    1040
................................................................................    1120
................................................................................    1200
.........................................................
//
NES-accession:	NES-0055
Date:		14 Aug 2002
Protein: 	Nucleoprotein p40 (N protein)
Organism:	Borna disease virus (BDV)
DB_reference:	TrEMBL Q91UL8
Necessity: 	-
Sufficiency: 	Aa 128-41 mediates export of GFP. L128A/L131A/I133A/I136A 
		abrogates export.
Pathway: 	LMB-sensitive.
Location: 	-
Regulation: 	BDV P protein masks the NES of p38 N protein, and prevent its 
		export.
Comments: 	This is the sequence of p40, p38 might be more correct.This
		sequence fits with the aa-numbering in the paper though. 
References:	Kobayashi T, Kamitani W, Zhang G, Watanabe M, Tomonaga K
		and Ikuta K, 2001,	
		J Virol 75: 3404-12. Medline: 11238866
Sequence:	370 aa       
MPPKRRLVDDADAMEDQDLYEPPASLPKLPGKFLQYTVGGSDPHPGIGHEKDIRQNAVALLDQSRRDMFHTVTPSLVFLC        80
LLIPGLHAAFVHGGVPRESYLSTPVTRGEQTVVKTAKFYGEKTTQRDLTELEISSIFSHCCSLLIGVVIGSSSKIKAGAE       160
QIKKRFKTMMAALNRPSHGETATLLQMFNPHEAIDWINGQPWVGSFVLSLLTTDFESPGKEFMDQIKLVASYAQMTTYTT       240
IKEYLAECMDATLTIPVVAYEIRDFLEVSAKLKEEHADLFPFLGAIRHPDAIKLAPRSFPNLASAAFYWSKKENPTMAGY       320
RASTIQPGASVKETQLARYRRREISRGEDGAELSGEISAIMKMIGVTGLN
................................................................................        80
...............................................MaaMaMaaMaaaaa...................       160
................................................................................       240
................................................................................       320
..................................................
//
NES-accession:	NES-0056
Date:		14 Aug 2002
Protein:	Zyxin	
Organism:	Gallus gallus (Chicken)
DB_reference:	SWISS-PROT Q04584, ZYX_CHICK
Necessity:	Delta 322-31 abrogates export of full-length Zyxin.
Sufficiency: 	Aa 319-35 mediates export of GST, whereas aa 322-31 does not.
Pathway:		-
Location: 	Colocalizes with integrin receptors at focal contacts.	
Regulation:	-
Comments:	-
References:	Nix DA and Beckerle MC, 1997,
		J Cell Biol 1138: 1139-47. Medline: 9281590
Sequence:	542 aa    
MASPGTPGTRMTTTVSINISTPSFYNPQKKFAPVVAPKPKVNPFKTGGTSESSQPQPPGTGAQRAQIGRVGEIPVSVTAE      80
ELPLPPPPPPGEELSFSSNCAFPPPPPPFEEPFPPAPDEAFPSPPPPPPPMFDEGPALQIPPGSTGSVEKPLAPKAHVEI     160
SSAPRDPTPPFPSKFTPKPSGTLSSKPPGLDSTPAPAPWAAPQQRKEPLASVPPPPSLPSQPTAKFTPPPVASSPGSKPG     240
ATVPMAPSNSTRYPTSLQTQFTAPSPSGPLSRPQPPNFTYAQQWERPQVQEKPVPTEKSAAVKDMRRPTADPPKGNSPLT     320
MKEVEELELLTQKLMKDMDHPPPVEAATSELCGFCRKPLSRTQPAVRALDCLFHVECFTCFKCEKQLQGQQFYNVDEKPF     400
CEDCYAGTLEKCSVCKQTITDRMLKATGNSYHPQCFTCVMCHTPLEGASFIVDQANQPHCVDDYHRKYAPRCSVCSEPIM     480
PEPGKDETVRVVALEKNFHMKCYKCEDCGRPLSIEADENGCFPLDGHVLCMKCHTVRAKTAC
................................................................................      80
................................................................................     160
................................................................................     240
...............................................................................a     320
aaaaaaaaaaaaaaa.................................................................     400
................................................................................     480
..............................................................
//
NES-accession:	NES-0057
Date:		14 Aug 2002
Protein:	Eukaryotic translation initiation factor 3 subunit 6 (eIF-3 p48)
		(Mammary tumor-associated protein INT-6) (Viral integration site
		protein INT-6)
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT Q64252, IF36_HUMAN
Necessity:	Aa 1-10 necessary in a deletional mutant context.
Sufficiency: 	Aa 1-20 mediates export of the nuclear protein DRBP76. 
		Export abrogated by either L6A, L14A or L18A. 
Pathway:		-
Location:	Cytoplasmic.			
Regulation:	-
Comments:	-
References:	Guo J and Sen GC, 2000,
		J Virol 74: 1892-9. Medline: 10644362
Sequence:	445 aa   
MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKNLYSDDIPHALREKRTTVVAQ      80
LKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADKHGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPA     160
TDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQP     240
QYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLV     320
NDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSP     400
YQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY
aaaaaMaaaaaaaMaaaMaa............................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
.............................................
//
NES-accession:	NES-0058
Date:		14 Aug 2002
Protein:	52 kDa immediate-early phosphoprotein (ORF 57)
Organism:	Herpesvirus saimiri (strain 11).
DB_reference:	SWISS-PROT P13199, IE63_HSVSA	
Necessity:	L164A/L166A abrogates export of full-length protein.
Sufficiency: 	Aa 154-170 mediates export of GFP.
Pathway:		-
Location:	-
Regulation:	-
Comments:	-
References:	Goodwin DJ, Hall KT, Stevenson AJ, Markham AF
		and Whitehouse A, 1999,
		J Virol 73: 10519-24. Medline: 10559371
Sequence:	417 aa   
MEDIIEGGISSDDDFDSSDSSSDEEESDTSPQIMKSDVTMASPPSTPEPSPDVSASTSNLKRERQRSPITWEHQSPLSRV      80
YRSPSPMRFGKRPRISSNSTSRSCKTSWADRVREAAAQRRPSRPFRKPYSHPRNGPLRNGPPRAPPLLKLFDISILPKSG     160
EPKLFLPVPSLPCQEAEKTNDKYVLAMAQRAMHDVPISSKQLTANLLPVKFKPLLSIVRYTPNYYYWVSMRKETIASANL     240
CTVAAFLDESLCWGQQYLKNDFIFSENGKDIILDTSSALLSQLVHKIKMLPFCHCLMQTTPQDHIVKQVCYLIASNNRIL     320
DAVRYLQTSVIKSPIVLLLAYAVCLPAAIICTKNETQLYSHCMRILKEYRPGDVMNILHESLTQHLNKCPSSTCAYTTRA     400
IVGTKANTTGLFFLPTQ
................................................................................      80
.........................................................................aaaaaaa     160
aaaMaMaaaa......................................................................     240
................................................................................     320
................................................................................     400
.................
//
NES-accession:	NES-0059
Date:		14 Aug 2002
Protein:	Importin beta-1 subunit (Karyopherin beta-1 subunit) 
		(Importin95) (Kap95p)
Organism:	Saccharomyces cerevisiae (Baker's yeast)
DB_reference:	SWISS-PROT Q06142, IMB1_YEAST
Necessity:	Aa 55-65 necessary in aa 1-77 fragment. L63A greatly impairs
		export of full-length Kap95p.
Sufficiency: 	Aa 1-77 mediates export of GST. 
Pathway:	-
Location:	-
Regulation:	-
Comments:	-
References:	Iovine MK and Wente SR, 1997
		J Cell Biol 137: 797-811. Medline: 9151683
Sequence:	861 aa    
MSTAEFAQLLENSILSPDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKLEGRILAALTLKNELVSKDSVKTQQF        80
AQRWITQVSPEAKNQIKTNALTALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVDNTGAEQPENVKRASLLALG       160
YMCESADPQSQALVSSSNNILIAIVQGAQSTETSKAVRLAALNALADSLIFIKNNMEREGERNYLMQVVCEATQAEDIEV       240
QAAAFGCLCKIMSLYYTFMKPYMEQALYALTIATMKSPNDKVASMTVEFWSTICEEEIDIAYELAQFPQSPLQSYNFALS       320
SIKDVVPNLLNLLTRQNEDPEDDDWNVSMSAGACLQLFAQNCGNHILEPVLEFVEQNITADNWRNREAAVMAFGSIMDGP       400
DKVQRTYYVHQALPSILNLMNDQSLQVKETTAWCIGRIADSVAESIDPQQHLPGVVQACLIGLQDHPKVATNCSWTIINL       480
VEQLAEATPSPIYNFYPALVDGLIGAANRIDNEFNARASAFSALTTMVEYATDTVAETSASISTFVMDKLGQTMSVDENQ       560
LTLEDAQSLQELQSNILTVLAAVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVFYAISALAASLGKGFEKYLETF       640
SPYLLKALNQVDSPVSITAVGFIADISNSLEEDFRRYSDAMMNVLAQMISNPNARRELKPAVLSVFGDIASNIGADFIPY       720
LNDIMALCVAAQNTKPENGTLEALDYQIKVLEAVLDAYVGIVAGLHDKPEALFPYVGTIFQFIAQVAEDPQLYSEDATSR       800
AAVGLIGDIAAMFPDGSIKQFYGQDWVIDYIKRTRSGQLFSQATKDTARWAREQQKRQLSL
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaMaaaaaaaaaaaaaaa..        80
................................................................................       160
................................................................................       240
................................................................................       320
................................................................................       400
................................................................................       480
................................................................................       560
................................................................................       640
................................................................................       720
................................................................................       800
.............................................................
//
NES-accession:	NES-0060
Date:		14 Aug 2002
Protein:	BR1 protein
Organism:	Squash leaf curl virus
DB_reference:	SWISS-PROT P21935, VBR1_SLCV
Necessity:	Aa 204-226 necessary in a deletional mutant context. Export of
		full-length BR1 is abrogated by L216A/L217A/L220A. 	
Sufficiency: 	Aa 204-283 mediates export of GUS-NLS.
Pathway:	-
Location:	-
Regulation:	-
Comments:	Discrepancy of 27 in amino acid numbering between article and
		retrieved sequence.
References:	Ward BM and Lazarowitz SG, 1999,
		Plant Cell 11: 1267-76. Medline: 10402428
Sequence:	283 aa    
MRRIHILDNTCIIYTYIHCAVAIASSLMYSTSNRRGRSQTQRGSHVRRTGVKRSYGAARGDDRRRPNVVSKTQVEPRMTI      80
QRVQENQFGPEFVLSQNSALSTFVTYPSYVKTVPNRTRTYIKLKRVRFKGTLKIERGQGDTIMDGPSSNIEGVFSMVIVV     160
DRKPHVSQSGRLHTFDELFGARIHCHGNLSVVPALKDRYYIRHVTKRVVSLEKDTLLIDLHGTTQLSNKRYNCWASFSDL     240
ERDSCNGVYGNITKNALLVYYCWLSDAQSKASTYVSFELDYLG
................................................................................      80
................................................................................     160
...........................................aaaaaaaaaaaaMMaaMaaaaaaaaaaaaaaaaaaaa     240
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
//
NES-accession:	NES-0061
Date:		14 Aug 2002
Protein:	Fragile X mental retardation 1 protein (Protein FMR-1) (FMRP)
Organism:	Homo sapiens (Human)
DB_reference:	SWISS-PROT Q06787, FMR1_HUMAN. 
Necessity:	Delta 425-41 results in nuclear localization.
Sufficiency: 	Aa 425-41 mediates export of BSA. Export is abrogated by
		L430A/L432A/L435A.
Pathway:	-
Location:	Cytoplasmic, ribosome-associated.	
Regulation:	-
Comments:	-
References:	Eberhart DE, Malter HE, Feng Y, Warren ST, 1996,
		Hum Mol Genet 5: 1083-91. Medline: 8842725
Sequence:	632 aa   
MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWW      80
LAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVT     160
YDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGAN     240
IQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGKVIGKNGKLIQEIVDKSGVVRVRIEA     320
ENEKNVPQEEEIMPPNSLPSNNSRVGPNAPEEKKHLDIKENSTHFSQPNSTKVQRVLVASSVVAGESQKPELKAWQGMVP     400
FVFVGTKDSIANATVLLDYHLNYLKEVDQLRLERLQIDEQLRQIGASSRPPPNRTDKEKSYVTDDGQGMGRGSRPYRNRG     480
HGRRGPGYTSGTNSEASNASETESDHRDELSDWSLAPTEEERESFLRRGDGRRRGGGGRGQGGRGRGGGFKGNDDHSRTD     560
NRPRNPREAKGRTTDGSLQIRVDCNNERSVHTKTLQNTSSEGSRLRTGKDRNQKKEKPDSVDGQQPLVNGVP
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
........................aaaaaMaMaaMaaaaaa.......................................     480
................................................................................     560
........................................................................
//
NES-accession:	NES-0062
Date:		14 Aug 2002
Protein: 	Transcriptional regulator IE63 (VMW63) (ICP27)
Organism:	Herpes simplex virus (type 1 / strain 17).
DB_reference: 	SWISS-PROT P10238, IE63_HSV11
Necessity: 	Delta aa 4-27 and L8R/L11R/L13R/L15R both abrogate export.
Sufficiency: 	Aa 5-17 mediates export of GFP.
Pathway: 	PKI-NES (NES-0001) can substitute, indicating common pathway.
Location: 	-
Regulation: 	-
Comments: 	-
References:	Sandri-Goldin RM, 1998,
		Genes Dev 12:868-79. Medline: 9512520
Sequence:	512 aa   
MATDIDMLIDLGLDLSDSDLDEDPPEPAESRRDDLESDSSGECSSSDEDMEDPHGEDGPEPILDAARPAVRPSRPEDPGV      80
PSTQTPRPTERQGPNDPQPAPHSVWSRLGARRPSCSPEQHGGKVARLQPPPTKAQPARGGRRGRRRGRGRGGPGAADGLS     160
DPRRRAPRTNRNPGGPRPGAGWTDGPGAPHGEAWRGSEQPDPPGGQRTRGVRQAPPPLMTLAIAPPPADPRAPAPERKAP     240
AADTIDATTRLVLRSISERAAVDRISESFGRSAQVMHDPFGGQPFPAANSPWAPVLAGQGGPFDAETRRVSWETLVAHGP     320
SLYRTFAGNPRAASTAKAMRDCVLRQENFIEALASADETLAWCKMCIHHNLPLRPQDPIIGTTAAVLDNLATRLRPFLQC     400
YLKARGLCGLDELCSRRRLADIKDIASFVFVILARLANRVERGVAEIDYATLGVGVGEKMHFYLPGACMAGLIEILDTHR     480
QECSSRVCELTASHIVAPPYVHGKYFYCNSLF
....aaaMaaMaMaMaa...............................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................
//
NES-accession:	NES-0063
Date:		14 Aug 2002
Protein: 	Nonstructural protein NS1
Organism:	Influenza A virus (strain A/Alaska/6/77).
DB_reference:	SWISS-PROT P03494, VNS1_IAALA
Necessity:	Aa 134-47 necessary in a deletional mutant context.  	
Sufficiency: 	Aa 138-47 mediates export of GST. Export abrogated by L144A and 
		L146A, whereas I145A had no effect.
Pathway: 	-
Location: 	Nuclear.
Regulation: 	NES masked by adjacent sequence, and NS1 (148-161). Exported
		upon unmasking (during infection).  
Comments: 	-
References:	Li Y, Yamakita Y and Krug RM, 1998,
		Proc Natl Acad Sci U S A 95: 4864-9. Medline: 9560194
Sequence:	237 aa   
MDSNTVSSFQVDCFLWHVRKQVVDQELGDAPFLDRLRRDQRSLRGRGSTLGLDIEAATHVGKQIVEKILKEESDEALKMT      80
MASTPASRYITDMTIEELSRDWFMLMPKQKVEGPLCIRMDQAIMEKNIMLKANFSVIFDRLETLILLRAFTEEGAIVGEI     160
SPLLSFPGHTIEDVKNAIGVLIGGLEWNDNTVRVSKTLQRFAWGSSNENGGPPLTPKQKRKMARTARSKVRRDKMAD
................................................................................      80
.........................................................aaaaaaMaMa.............     160
.............................................................................
//
NES-accession:	NES-0064
Date:		14 Aug 2002
Protein: 	MAP kinase kinase 6 (MKK6)
Organism:	Cyprinus carpio (Common carp)
DB_reference:	TrEMBL Q9I959
Necessity: 	Both delta1-57, L46A/L50A and L53A/L55A abrogate export of MKK6.
Sufficiency: 	Aa 45-57 mediates export of ovalbumin. 
		Export abrogated by L46A/L50A/L53A/L55A.
Pathway: 	-
Location: 	Cytoplasmic.
Regulation: 	-
Comments: 	-
References:	Hashimoto H, Fukuda M, Matsuo Y, Yokoyama Y, Nishida E, 
		Toyohara H and Sakaguchi M, 2000,
		Eur J Biochem 2000 267: 4362-71. Medline: 10880959
Sequence:	404 aa    
MMGHACGSSASDLLQMERTYLALLSRLPLSHLHSLSSHSLALSHLLSQDLEPLALSDRKCSSDRESRNSMGSRNKAGKKK        80
PPLKLPKEVFEKPQPAPTPPRDLDSKACVTIGDKNFVVKADDLEQIGELGRGAYGVVDKMRHVPSGVIMAVKRIRATVNT       160
QEQKRLLMDLDISMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYKQVHEKGMTIPEDILGKITVSIVKALEHLH       240
SNLSVIHRDVKPSNVLINMQGQVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPETNQKGYNVKSDIWSLGITMIEL       320
AILRFPYDSWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTSQCLRKNSKERPTYTELMQHPFFTIHDSKDTDVASFVKS       400
ILGD
............................................aMaaaMaaMaMaa.......................        80
................................................................................       160
................................................................................       240
................................................................................       320
................................................................................       400
....
//
NES-accession:	NES-0065
Date:		14 Aug 2002
Protein:	LIM protein SLIMMER
Organism:	Homo sapiens (Human)
DB_reference:	TrEMBL O95212
Necessity:	-
Sufficiency: 	Aa 254-65 sufficient in a deletional mutant context.
Pathway:		-
Location:	Nuclear in myoblasts and cytoplasmic in myotubes	
Regulation:	-
Comments:	-
References:	Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM and
		Mitchell CA, 1999,
		J Biol Chem 274: 27083-91. Medline: 10480922
Sequence:	323 aa    
MAEKFDCHYCRDPLQGKKYVQKDGHHCCLKCFDKFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETF        80
VAKDNKILCNKCTTREDSPKCKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQVIGTGSFFPKGEDFYCVTCHETKFAK       160
HCVKCNKAITSGGITYQDQPWHADCFVCVTCSKKLAGQRFTAVEDQYYCVDCYKNFVAKKCAGCKNPITGKRTVSRVSHP       240
VSKARKPPVCHGKRLPLTLFPSANLRGRHPGGERTCPSWVVVLYRKNRSLAAPRGPGLVKAPVWWPMKDNPGTTTASTAK       320
NAP
................................................................................        80
................................................................................       160
................................................................................       240
..............aaaaaaaaaaa.......................................................       320
...
//
NES-accession:	NES-0066
Date:		14 Aug 2002
Protein:	Homeobox protein engrailed-2 (Gg-En-2)
Organism:	Gallus gallus (Chicken)
DB_reference:	SWISS-PROT Q05917, HME2_CHICK
Necessity:	Delta 232-43 impaires export, but shuttling is still detected
		in 20% of heterokaryons.
Sufficiency: 	Aa 229-48 mediates export of reporterprotein.  
Pathway:	-
Location:	Nuclear	
Regulation:	-
Comments:	-
References:	Maizel A, Bensaude O, Prochiantz A, Joliot A, 1999,
		Development 126: 3183-90. Medline: 10375508
Sequence:	288 aa 
MEEGGRSPREEAAEPQESGGDAEPGGGRRALLLPPGDPPHPHPHPHRITNFFIDNILRPEFGRRKEAGGTAGEPRRPGAE      80
SRRSPAAAAPAPGAPVPGGGGGGGGGSPGRGEGGPAALALHGAAKKGGDPAALEAALKARGLSGAELSVSSDSDSSQAGS     160
NAGNQPMLWPAWVYCTRYSDRPSSPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELGLNE     240
SQIKIWFQNKRAKIKKATGSKNSLAVHLMAQGLYNHSTTAKDGKSDSE
................................................................................      80
................................................................................     160
....................................................................aaaaaaaaaaaa     240
aaaaaaaa........................................
//
NES-accession:	NES-0067
Date:		14 Aug 2002
Protein:	Nonsense-mediated mRNA decay protein 3 (Nmd3p)
Organism:	Saccharomyces cerevisiae (Baker's yeast)
DB_reference:	SWISS-PROT P38861, NMD3_YEAST
Necessity:	Delta 474-518 greatly impairs export, while delta 439-518
		completely abrogates export.
Sufficiency: 	Aa 435-518 mediates export of NLS-GFP fusion. 
Pathway:	LMB-sensitive, Crm-dependent.
Location:	Cytoplasmic.
Regulation:	-
Comments:	The assignment field for this sequenceis not accurate:
		The experimental data propose 2 NESs, located from aa 445-56 
		and 492-503, respectively.		 
References:	Ho JH, Kallstrom G, Johnson AW, 2000,
		J Cell Biol 151: 1057-66. Medline: 11086007
		Gadal O, Strauss D, Kessl J, Trumpower B, Tollervey D
		and Hurt E, 2001,
		Mol Cell Biol 21: 3405-15. Medline: 11313466
Sequence:	518 aa   
MEFTPIDPHQHQNAATLLCCNCGTPIDGSTGLVMCYDCIKLTVDITQGIPREANISFCRNCERFLQPPGQWIRAELESRE      80
LLAICLRRLKGLTFVRLVDASFIWTEPHSRRIRIKLTVQGEAMTNTIIQQTFEVEYIVIAMQCPDCARSYTTNTWRATVQ     160
IRQKVPHKRTFLFLEQLILKHNAHVDTISISEAKDGLDFFYAQKNHAVKMIDFLNAVVPIKHKKSEELISQDTHTGASTY     240
KFSYSVEIVPICKDDLVVLPKKLAKSMGNISQFVLCSKISNTVQFMDPTTLQTADLSPSVYWRAPFNALADVTQLVEFIV     320
LDVDSTGISRGNRVLADITVARTSDLGVNDQVYYVRSHLGGICHAGDSVMGYFIANSNYNSDLFDGLNIDYVPDVVLVKK     400
LYQRKSKKSRHWKLKRMAKEHKDIDASLDYNSRAQKQEMERAEKDYELFLQELEEDAELRQSVNLYKNREANVPPEEHEM     480
DEDEDEDAPQINIDELLDELDEMTLEDGVENTPVESQQ
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
..................................aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa     480
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
//
NES-accession:	NES-0068
Date:		14 Aug 2002
Protein: 	Forkhead box protein O3A (Forkhead in rhabdomyosarcoma-like 1)
		(AF6q21 protein) (FKHRL1)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT O43524, FXO3_HUMAN
Necessity: 	Export abrogated by simultaneous mutation of both putative NESs 
		(369-78 and 386-96) by M376A/L378A/L382A/L390A/L391A/I394A/L396A.
Sufficiency:	-
Pathway: 	LMB-sensitive.
Location: 	Nuclear.
Regulation: 	Exported upon phosphorylation by Akt and only when bound to 
		14-3-3 protein (the latter shown not to be providing the NES).
Comments: 	-
References: 	Brunet A, Kanai F, Stehn J, Xu J, Sarbassova D, Frangioni JV,
		Dalal SN, DeCaprio JA, Greenberg ME, Yaffe MB, 2002
		J Cell Biol 156: 817-28. Medline: 11864996
Sequence:	673 aa   
MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIG      80
GGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNL     160
SYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDG     240
GKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTV     320
SGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQ     400
PSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLS     480
HSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLS     560
ESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDF     640
NFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
.......................................................M.M...M.......MM..M.M....     400
................................................................................     480
................................................................................     560
................................................................................     640
.................................
//
NES-accession:	NES-0069
Date:		14 Aug 2002
Protein: 	Histone deacetylase 5 (HD5) (Antigen NY-CO-9) (HDAC5)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT Q9UQL6, HDA5_HUMAN
Necessity: 	Delta 1081-1122, V1086 and L1092A all greatly impaires 
		or abrogates CaMK-mediated nuclear export.
Sufficiency: 	Aa 1081-22 NOT suffcient to mediate export of GFP, but suffcient
		to mediate export of MITR - another 14-3-3 binding protein -
		upon CaMK stimulation.
Pathway: 	LMB-sensitive.
Location: 	Nuclear.
Regulation: 	Export upon phosphorylation by CaMK.
Comments: 	Results indicative of additional sequence information being
		necessary for export mediated by this NES.
References:	McKinsey TA, Zhang CL and Olson EN, 2001,
		Mol Cell Biol 21: 6312-21. Medline: 11509672
Sequence:	1122 aa   
MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQEL        80
LALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRL       160
EQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKL       240
PLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPS       320
SPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAE       400
RQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATS       480
MRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQ       560
EVLLGEGALTMPREGSTESESTQEDLEEEDEEEDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQ       640
VYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQE       720
TGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSA       800
VRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQA       880
FYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVS       960
AGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPN      1040
INAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEP      1120
AL
................................................................................        80
................................................................................       160
................................................................................       240
................................................................................       320
................................................................................       400
................................................................................       480
................................................................................       560
................................................................................       640
................................................................................       720
................................................................................       800
................................................................................       880
................................................................................       960
................................................................................      1040
........................................aaaaaMaaaaaMaaaaaaaaaaaaaaaaaaaaaaaaaaaa      1120
aa
//
NES-accession:	NES-0070
Date:		14 Aug 2002
Protein: 	Histone deacetylase 4 (HD4) (HA6116) (HDAC4)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT P56524, HDA4_HUMAN
Necessity: 	V1056A greatly impaires, whereas L1062 abrogates the CaMK 
		mediated export (McKinsey et al. 2001). 
		Aa 1040-84 necessary in a deletional mutant context (Wang et al.
		2001).
Sufficiency: 	Aa 1044-69 mediates export of GFP, but this export is abrogated 
		by either M1059A, L1062A, V1064A or V1066A (Wang et al. 2001).
Pathway: 	LMB-sensitivity. 
Location: 	Nuclear.
Regulation: 	Exported upon phosphorylation by CaMK (McKinsey et al. 
		2001).
Comments:  	-
References: 	McKinsey TA, Zhang CL and Olson EN, 2001,
		Mol Cell Biol 21: 6312-21. Medline: 11509672
		Wang AH and Yang XJ, 2001,
		Mol Cell Biol 21: 5992-6005. Medline: 11486037
Sequence:	1084 aa  
MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQLQQELLALKQ      80
KQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEK     160
GKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFP     240
LRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDVTDSACSSAPGSGPSSPNNSSGSVSAENGIAPAV     320
PSIPAETSLAHRLVAREGSAAPLPLYTSPSLPNITLGLPATGPSAGTAGQQDTERLTLPALQQRLSLFPGTHLTPYLSTS     400
PLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHL     480
VIQQQHQQFLEKHKQQFQQQQLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEAHAQAGVQVKQ     560
EPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGGHRPLSRAQSSPASATFP     640
VSVQEPPTKPRFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHT     720
LLYGTNPLNRQKLDSKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPP     800
GHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEV     880
GTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQL     960
MGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGR    1040
SLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
................................................................................     720
................................................................................     800
................................................................................     880
................................................................................     960
................................................................................    1040
...aaaaaaaaaaaaaaaMaaMaMaMaaa...............
//
NES-accession:	NES-0071
Date:		14 Aug 2002
Protein: 	Transcription factor E2F4 (E2F-4)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT Q16254, E2F4_HUMAN
Necessity: 	I68A/L70A, delta 84-105 and L97S/I98R all lead to nuclear 
		accumulation, which is abolished by overexpression of Crm1.
Sufficiency: 	Since neither of the above-mentioned mutants are cytoplasmic, 
		it seems that they are not 2 independently sufficient NESs.
Pathway:	LMB-sensitive, Crm-dependent.
Location: 	Cytoplasmic.
Regulation: 	-
Comments: 	Atypical long NES.
References:	Gaubatz S, Lees JA, Lindeman GJ, Livingston DM, 2001,
		Mol Cell Biol 21: 1384-92. Medline: 11158323
Sequence:	413 aa    
MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQ      80
WKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAP     160
SGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEAS     240
RPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSN     320
SNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESE     400
GVCDLFDVPVLNL
...................................................................M.M..........      80
................MM..............................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
.............
//
NES-accession:	NES-0072
Date:		14 Aug 2002
Protein: 	DNA damage checkpoint protein rad24
Organism:	Schizosaccharomyces pombe (Fission yeast)
DB_reference: 	SWISS-PROT P42656, RA24_SCHPO
Necessity: 	I222A/L226A greatly impairs export.
Sufficiency:	-
Pathway: 	Crm-dependency.
Location: 	Cytoplasmic.
Regulation: 	-
Comments: 	-
References:	Lopez-Girona A, Furnari B, Mondesert O and Russell P, 1999,
		Nature 397: 172-5. Medline: 9923681
Sequence:	270 aa   
MSTTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQ      80
VELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEI     160
ATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAEYS     240
AAAAGGNTEGAQENAPSNAPEGEAEPKADA
................................................................................      80
................................................................................     160
.............................................................M...M..............     240
..............................
//
NES-accession:	NES-0073
Date:		14 Aug 2002
Protein: 	Serine/threonine protein kinase 4 (STE20-like kinase MST1)
		(MST-1) (Mammalian STE20-like protein kinase 1) 
		(Serine/threonine protein kinase Krs-2)
Organism:	Homo sapiens (Human)
DB_reference: 	SWISS-PROT Q13043, STK4_HUMAN
Necessity: 	Only simultaneous mutation of both NESs abrogates export
		(M368A/I370A/W439A/V441A or M368A/I370A/V441A/L444A).
Sufficiency: 	The fact that MST1 is still excluded from the nucleus when only
		1 NES is mutated at the time, is indicative of each NES being 
		sufficient.
Pathway: 	LMB-sensitive.
Location: 	Cytoplasmic.
Regulation: 	Dimerization facilitates cytoplasmic retention, whereas 
		phosphorylation stimulates import.
Comments: 	-
References:	Lee KK and Yonehara S, 2002,
		J Biol Chem 277: 12351-8. Medline: 11805089
Sequence:	487 aa   
METVQLRNPPRRQLKKLDEDSLTKQPEEVFDVLEKLGEGSYGSVYKAIHKETGQIVAIKQVPVESDLQEIIKEISIMQQC      80
DSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYLHFMRKIHRDIKAGNILLNTE     160
GHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNP     240
PPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPFVRSAKGVSILRDLINEAMDVKLKRQESQQREVDQDDEENS     320
EEDEMDSGTMVRAVGDEMGTVRVASTMTDGANTMIEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFE     400
QKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAK     480
KRRQQNF
................................................................................      80
................................................................................     160
................................................................................     240
................................................................................     320
...............................................M.M..............................     400
......................................M.M..M....................................     480
.......
//
NES-accession:	NES-0074
Date:		14 Aug 2002
Protein: 	Iron-regulated transcriptional repressor AFT1
Organism:	Saccharomyces cerevisiae (Baker's yeast)
DB_reference: 	SWISS-PROT P22149, AFT1_YEAST. NLSdb NLS:270
Necessity: 	L99A and L102A constitutively nuclear even in iron-replete cells.
Sufficiency:	-
Pathway: 	-
Location: 	Nuclear in iron-deplete cells, and cytoplasmic in iron-replete
		cells.
Regulation: 	-
Comments: 	-
References:	Yamaguchi-Iwai Y, Ueta R, Fukunaka A, Sasaki R, 2002,
		J Biol Chem 277: 18914-8. Medline: 11877447
Sequence:	690 aa   
MEGFNPADIEHASPINSSDSHSSSFVYALPKSASEYVVNHNEGRASASGNPAAVPSPIMTLNLKSTHSLNIDQHVHTSTS      80
PTETIGHIHHVEKLNQNNLIHLDPVPNFEDKSDIKPWLQKIFYPQGIELVIERSDAFKVVFKCKAAKRGRNARRKRKDKP     160
KGQDHEDEKSKINDDELEYASPSNATVTNGPQTSPDQTSSIKPKKKRCVSRFNNCPFRVRATYSLKRKRWSIVVMDNNHS     240
HQLKFNPDSEEYKKFKEKLRKDNDVDAIKKFDELEYRTLANLPIPTATIPCDCGLTNEIQSFNVVLPTNSNVTSSASSST     320
VSSISLDSSNASKRPCLPSVNNTGSINTNNVRKPKSQCKNKDTLLKRTTMQNFLTTKSRLRKTGTPTSSQHSSTAFSGYI     400
DDPFNLNEILPLPASDFKLNTVTNLNEIDFTNIFTKSPHPHSGSTHPRQVFDQLDDCSSILFSPLTTNTNNEFEGESDDF     480
VHSPYLNSEADFSQILSSAPPVHHDPNETHQENQDIIDRFANSSQEHNEYILQYLTHSDAANHNNIGVPNNNSHSLNTQH     560
NVSDLGNSLLRQEALVGSSSTKIFDELKFVQNGPHGSQHPIDFQHVDHRHLSSNEPQVRSHQYGPQQQPPQQLQYHQNQP     640
HDGHNHEQHQTVQKDMQTHESLEIMGNTLLEEFKDIKMVNGELKYVKPED
................................................................................      80
..................M..M..........................................................     160
................................................................................     240
................................................................................     320
................................................................................     400
................................................................................     480
................................................................................     560
................................................................................     640
..................................................
//
NES-accession:	NES-0075
Date:		14 Aug 2002
Protein:	Transcriptional regulator IE63 homolog (Diffuse early antigen)
		(SM) (BMLF1)
Organism:	Epstein-barr virus (strain B95-8) (Human herpesvirus 4)
DB_reference:	SWISS-PROT Q04360, IE63_EBV
Necessity:	Delta 227-36 and V184A/T185A/L186A/L193A/T194A/L195A both
		abrogate shuttling in heterokaryon assay, whereas
		L193A/T194A/L195A does not.
Sufficiency: 	Aa 186-95 and aa 177-86 are each independently sufficient to
		mediate export of a GFP-NLS-PK fusion protein. 
Pathway:	LMB sensitive.
Location: 	Nuclear.	
Regulation:	-
Comments:	2 overlapping NESs (designated "A/M" and "a/M", respectively). 
		Discrepancy of 41 in amino acid numbering between article and 
		retrieved sequence.
References:	Chen L, Liao G, Fujimuro M, Semmes OJ and Hayward SD, 2001,
		Virology 288: 119-28. Medline: 11543664
Sequence:	438 aa 
MEGSEEHSTDGEISSSEEEDEDPTPAHAIPARPSSVVITPTSASFVIPRKKWDLQDKTVTLHRSPLCRDEDEKEETGNSS      80
YTRGHKRRRGEVHGCTDESYGKRRHLPPGARAPRAPRAPRVPRAPRSPRAPRSNRATRGPRSESRGAGRSTRKQARQERS     160
QRPLPNKPWFDMSLVKPVSKITFVTLPSPLASLTLEPIQDPFLQSMLAVAAHPEIGAWQKVQPRHELRRSYKTLREFFTK     240
STNKDTWLDARMQAIQNAGLCTLVAMLEETIFWLQEITYHGDLPLAPAEDILLACAMSLSKVILTKLKELAPCFLPNTRD     320
YNFVKQLFYITCATARQNKVVETLSSSYVKQPLCLLAAYAAVAPAYINANCRRRHDEVEFLGHYIKNYNPGTLSSLLTEA     400
VETHTRDCRSASCSRLVRAILSPGTGSLGLFFVPGLNQ
................................................................................      80
................................................................................     160
................AAAAAAAMMMaaaaaaMMM.............................................     240
................................................................................     320
................................................................................     400
......................................
//